Confocal fluorescence microscopy is the most widely used method for advanced light microscopic imaging of biological specimens. However, several new technologies now permit fluorescence microscopic imaging that is either faster than standard (point-scanning) confocal microscopy, has better spatial resolution, or both. Rapid image collection techniques, which are ideal for examining live cells, tissues, and organisms, including swept field, multiphoton, and light-sheet microscopy will be highlighted at the workshop. Super-resolution techniques, which can result in 2- to 10-fold improvement in spatial resolution relative to standard confocal microscopy, including gated STED, single-molecule localization, and Airyscan microscopy, will also be discussed. The workshop will illustrate the use of these new imaging techniques, each of which is now available in the medical school’s Center for Cell and Molecular Imaging (CCMI).
Date
Tuesday, March 12, 2019
Time
1:00 P.M. until 5:00 P.M.
Location
The Anlyan Center, N107
300 Cedar Street
New Haven, CT
Speakers:
Michael Nathanson, MD, PhD
Gladys Phillips Crofoot Professor of Medicine (Digestive Diseases) and Professor of Cell Biology; Director, Yale Liver Center; Director, Center for Cell and Molecular Imaging
Engin Deniz, MD
Assistant Professor of Pediatrics (Critical Care)
Emilie Guillon
Postdoctoral Associate in Molecular, Cellular, and Developmental Biology
Lena Schroeder
Associate Research Scientist in Cell Biology
Joseph Santos-Sacchi, PhD
Professor of Surgery (Otolaryngology), of Cellular and Molecular Physiology and of Neuroscience
Wasim Sayyad
Postdoctoral Associate in Molecular, Cellular, and Developmental Biology
Ting (Chao-ting) Wu, PhD
Professor of Genetics
Harvard Medical School