Development of New Single-Molecule Methods

We have been developing new instruments or upgrading our machines by combining high-resolution optical tweezers, single-molecule fluorescence detection, and microfluidics to better study single proteins or protein complexes. We have been also developing new methods or algorithms to analyze data from single molecule experiments.

G. Sirinakis, Y. X. Ren, Y. Gao, Z. Q. Xi, Y. L. Zhang, Combined and versatile high-resolution optical tweezers and single-molecule fluorescence microscopy. Rev Sci Instrum. 83: 093708-(1-9) (2012).

Y. L. Zhang, J. Jiao, A. A. Rebane, Hidden Markov modeling with detailed balance and its application to single protein folding Biophys J 111, 2110 (2016).

A. A. Rebane, L. Ma, Y. L. Zhang, Structure-based derivation of protein folding intermediates and energies from optical tweezers. Biophys J 110, 441 (2016).