Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution
Mudumbi K, Schirmer E, Yang W. Single-point single-molecule FRAP distinguishes inner and outer nuclear membrane protein distribution. Nature Communications 2016, 7: 12562. PMID: 27558844, PMCID: PMC5007294, DOI: 10.1038/ncomms12562.Peer-Reviewed Original ResearchConceptsNuclear envelope transmembrane proteinsInner nuclear membraneOuter nuclear membraneNuclear membraneEnvelope transmembrane proteinsMembrane protein distributionTranslocation rateTransmembrane proteinEndoplasmic reticulumProtein distributionHuman diseasesMembraneProteinReticulumMicroscopy techniquesDetermination of distributionVivoProbing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP
Mudumbi K, Yang W. Probing Protein Distribution Along the Nuclear Envelope In Vivo by Using Single-Point FRAP. Methods In Molecular Biology 2016, 1411: 113-122. PMID: 27147037, PMCID: PMC10099394, DOI: 10.1007/978-1-4939-3530-7_6.Peer-Reviewed Original ResearchConceptsNuclear envelope transmembrane proteinsNuclear membrane proteinsEnvelope transmembrane proteinsInner nuclear membraneNuclear envelope membranesWeeks-long processEnvelope membraneMembrane proteinsTransmembrane proteinNuclear envelopeNuclear membraneProtein distributionFluorescence recoveryStaining of cellsProteinMembraneElectron microscopy