2023
In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex
You X, Zhang X, Cheng J, Xiao Y, Ma J, Sun S, Zhang X, Wang H, Sui S. In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature 2023, 616: 199-206. PMID: 36922595, DOI: 10.1038/s41586-023-05831-0.Peer-Reviewed Original ResearchConceptsPhotosystem IOxygenic photosynthetic organismsSolid structural basisSitu structureLight-harvesting complexRed alga Porphyridium purpureumPhotosynthetic organismsIncomplete proteinStructural basisPhycobilisomesExcitation energy transfer pathwaysMegacomplexesPorphyridium purpureumPSIIPhotosystemsElectron tomographyLight energyAtomic resolutionStructural informationInteraction detailsAssemblyTransfer pathwayEnergy transfer pathwaysUnclear owingOrganismsDetermining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA
Cheng J, Liu T, You X, Zhang F, Sui S, Wan X, Zhang X. Determining protein structures in cellular lamella at pseudo-atomic resolution by GisSPA. Nature Communications 2023, 14: 1282. PMID: 36922493, PMCID: PMC10017804, DOI: 10.1038/s41467-023-36175-y.Peer-Reviewed Original ResearchMeSH KeywordsCryoelectron MicroscopyElectron Microscope TomographyImage Processing, Computer-AssistedConceptsProtein complexesCellular lamellaTarget protein complexesCryo-electron tomographyProtein structure determinationCryo-electron microscopy imagesHigh-throughput data collectionProtein structureAtomic resolutionStructure determinationComplexesPhycobilisomesComplex structureMajor toolLamellaeHigh-resolution reconstruction
2019
High-quality, high-throughput cryo-electron microscopy data collection via beam tilt and astigmatism-free beam-image shift
Wu C, Huang X, Cheng J, Zhu D, Zhang X. High-quality, high-throughput cryo-electron microscopy data collection via beam tilt and astigmatism-free beam-image shift. Journal Of Structural Biology 2019, 208: 107396. PMID: 31562921, DOI: 10.1016/j.jsb.2019.09.013.Peer-Reviewed Original Research