2009
Reductive activation of the prodrug 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)-2-[[1-(4-nitrophenyl)ethoxy]carbonyl]hydrazine (KS119) selectively occurs in oxygen-deficient cells and overcomes O6-alkylguanine-DNA alkyltransferase mediated KS119 tumor cell resistance
Baumann RP, Penketh PG, Ishiguro K, Shyam K, Zhu YL, Sartorelli AC. Reductive activation of the prodrug 1,2-bis(methylsulfonyl)-1-(2-chloroethyl)-2-[[1-(4-nitrophenyl)ethoxy]carbonyl]hydrazine (KS119) selectively occurs in oxygen-deficient cells and overcomes O6-alkylguanine-DNA alkyltransferase mediated KS119 tumor cell resistance. Biochemical Pharmacology 2009, 79: 1553-1561. PMID: 20005211, PMCID: PMC2885764, DOI: 10.1016/j.bcp.2009.12.004.Peer-Reviewed Original ResearchConceptsAlkylguanine-DNA alkyltransferaseDNA GO6-alkylguanine-DNA alkyltransferaseCell linesBase-catalyzed activationCellular protectionReductive activationCross-link lesionsTumor cell resistanceCultured cellsCell lethalityReductase enzymePosition of guanineDifferent tumor typesIntracellular activationAlkyltransferaseCell resistanceActivationEnzymeCytotoxic effectsCellsOxygen-deficient cellsHypoxic cellsOxygen-deficient conditions
2001
Acquisition of Lubrol Insolubility, a Common Step for Growth Hormone and Prolactin in the Secretory Pathway of Neuroendocrine Cells*
Lee M, Zhu Y, Chang J, Dannies P. Acquisition of Lubrol Insolubility, a Common Step for Growth Hormone and Prolactin in the Secretory Pathway of Neuroendocrine Cells*. Journal Of Biological Chemistry 2001, 276: 715-721. PMID: 11024038, DOI: 10.1074/jbc.m008530200.Peer-Reviewed Original ResearchAnimalsAnti-Bacterial AgentsBrefeldin AChloroquineCOS CellsDinitrobenzenesEndoplasmic ReticulumEpidermal Growth FactorEstradiolHuman Growth HormoneHydrogen-Ion ConcentrationInsulinMacrolidesMutationPituitary GlandPolyethylene GlycolsProlactinProtein TransportRatsSecretory VesiclesSolubilitySubstrate SpecificityTumor Cells, CulturedUltracentrifugation
2000
Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor
Lee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 2000, 141: 3485-3492. PMID: 10965922, DOI: 10.1210/endo.141.9.7647.Peer-Reviewed Original ResearchConceptsSecretory pathwayEpidermal growth factorSynaptotagmin IIICyclophilin BGH4C1 cellsInduction of proteinsGrowth factorGlucose-regulated protein 94Rat pituitary GH4C1 cellsSecretory granulesSynaptosomal-associated proteinMessenger RNA accumulationMembrane proteinsPituitary GH4C1 cellsSynaptosomal associated proteinRNA accumulationPlasma membraneProtein AssociatedSpecific proteinsSynaptotagmin IHormone-treated cellsSNAP-25Protein 94Secretory granule accumulationCoordinate event