2020
Impaired hypocretin/orexin system alters responses to salient stimuli in obese male mice
Tan Y, Hang F, Liu ZW, Stoiljkovic M, Wu M, Tu Y, Han W, Lee AM, Kelley C, Hajos M, Lu L, de Lecea L, de Araujo I, Picciotto M, Horvath TL, Gao XB. Impaired hypocretin/orexin system alters responses to salient stimuli in obese male mice. Journal Of Clinical Investigation 2020, 130: 4985-4998. PMID: 32516139, PMCID: PMC7456212, DOI: 10.1172/jci130889.Peer-Reviewed Original ResearchConceptsHcrt cellsObese miceDiet-induced obese miceObese male miceExcessive energy intakeNeuropeptide hypocretin/orexinHypocretin/orexinHcrt neuronsMale miceHcrt systemClinical studiesCommon causeSynaptic transmissionObese animalsEnergy intakeAcute stressCognitive functionSalient stimuliAlters responsesExact mechanismMiceHomeostatic regulationNeuronal networksBehavioral changesNeurons
2019
A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement
Mor A, Zhang M, Esencan E, Simsek B, Nichols-Burns SM, Liu Y, Lo J, Kelk DA, Flores V, Gao XB, Seli E. A step towards the automation of intracytoplasmic sperm injection: real time confirmation of mouse and human oocyte penetration and viability by electrical resistance measurement. Fertility And Sterility 2019, 113: 234-236. PMID: 31883732, DOI: 10.1016/j.fertnstert.2019.09.023.Peer-Reviewed Original ResearchConceptsIntracytoplasmic sperm injectionICSI pipetteSperm injectionOocyte penetrationIntact oolemmaOocyte retrieval procedureMann-Whitney testMouse oocytesOocyte retrievalFemale miceMAIN OUTCOMEAcademic centersImmature oocytesHuman oocytesMature oocytesObjective toolMiceOocytesWomenVisual assessmentPipetteInjectionOolemmaStep 1Group
2010
Direct Evidence for Wake-Related Increases and Sleep-Related Decreases in Synaptic Strength in Rodent Cortex
Liu ZW, Faraguna U, Cirelli C, Tononi G, Gao XB. Direct Evidence for Wake-Related Increases and Sleep-Related Decreases in Synaptic Strength in Rodent Cortex. Journal Of Neuroscience 2010, 30: 8671-8675. PMID: 20573912, PMCID: PMC2903226, DOI: 10.1523/jneurosci.1409-10.2010.Peer-Reviewed Original ResearchConceptsMiniature EPSCsAmplitude of mEPSCsFrontal cortex slicesNet synaptic potentiationCerebral cortexCortex slicesLarge brain areasSynaptic potentiationSynaptic currentsBrain areasRodent cortexStrong synapsesRecovery sleepSynaptic homeostasisSynaptic strengthSleepCortexSynapsesEPSCsTime of dayRatsPotentiationMiceDirect evidence
2007
Prolonged wakefulness induces experience-dependent synaptic plasticity in mouse hypocretin/orexin neurons
Rao Y, Liu ZW, Borok E, Rabenstein RL, Shanabrough M, Lu M, Picciotto MR, Horvath TL, Gao XB. Prolonged wakefulness induces experience-dependent synaptic plasticity in mouse hypocretin/orexin neurons. Journal Of Clinical Investigation 2007, 117: 4022-4033. PMID: 18060037, PMCID: PMC2104495, DOI: 10.1172/jci32829.Peer-Reviewed Original ResearchConceptsHypocretin/orexin neuronsLong-term potentiationOrexin neuronsGlutamatergic synapsesSynaptic plasticitySleep lossExperience-dependent synaptic plasticityDopamine D1 receptorsChronic sleep lossSleep-wake regulationModafinil treatmentLateral hypothalamusD1 receptorsSimilar potentiationBrain slicesNeuronal activityNeuronal circuitryDopamine systemNervous systemSynaptic strengthNeuronsPathological conditionsGentle handlingMiceWakefulness
2005
Input organization and plasticity of hypocretin neurons Possible clues to obesity’s association with insomnia
Horvath TL, Gao XB. Input organization and plasticity of hypocretin neurons Possible clues to obesity’s association with insomnia. Cell Metabolism 2005, 1: 279-286. PMID: 16054072, DOI: 10.1016/j.cmet.2005.03.003.Peer-Reviewed Original ResearchConceptsHypocretin neuronsSynaptic currentsHypothalamic hypocretin neuronsMore excitatory synapsesOvernight food deprivationElevated food intakeExcitatory synaptic currentsControl of arousalCause of narcolepsyStress-induced plasticityHypocretin levelsLeptin administrationHypocretin cellsHypocretin signalingMetabolic disturbancesObesity associationSynaptic organizationExcitatory synapsesFood intakeInhibitory inputsCell bodiesUnderlying causeNeuronsInput organizationFood deprivation
1998
The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity
de Lecea L, Kilduff TS, Peyron C, Gao X, Foye PE, Danielson PE, Fukuhara C, Battenberg E, Gautvik VT, Bartlett FS, Frankel WN, van den Pol AN, Bloom FE, Gautvik KM, Sutcliffe JG. The hypocretins: Hypothalamus-specific peptides with neuroexcitatory activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 322-327. PMID: 9419374, PMCID: PMC18213, DOI: 10.1073/pnas.95.1.322.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCarrier ProteinsChromosomesConsensus SequenceHomeostasisHypothalamus, PosteriorIntracellular Signaling Peptides and ProteinsMaleMiceMolecular Sequence DataNeuronsNeuropeptidesNeurotransmitter AgentsOrexinsProtein PrecursorsRatsRats, WistarSecretinSynaptic VesiclesConceptsGut hormone secretinLateral hypothalamic areaNeuronal cell bodiesLarge granular vesiclesPosterior hypothalamusHypothalamic areaHypothalamic neuronsHippocampal neuronsCell bodiesGranular vesiclesHcrt peptidesHormone secretinNeuronsHypocretinNeuroexcitatory activityPeptidesPutative precursorBrainstemThalamusHypothalamusImmunoreactivityCNSNeurotransmittersSecretinProtein products