2019
Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice
Xavier-Ferrucio J, Scanlon V, Li X, Zhang PX, Lozovatsky L, Ayala-Lopez N, Tebaldi T, Halene S, Cao C, Fleming MD, Finberg KE, Krause DS. Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice. Blood 2019, 134: 1547-1557. PMID: 31439541, PMCID: PMC6839952, DOI: 10.1182/blood.2019002039.Peer-Reviewed Original ResearchConceptsMK lineage commitmentExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayMegakaryocytic-erythroid progenitorsBone marrow transplantation assaysSignal transduction analysisIron-deficient conditionsGene expression analysisMegakaryocytic commitmentLineage commitmentTransferrin receptor 2MK lineageTmprss6-/- miceIron sensorExpression analysisKinase pathwayTransduction analysisTransplantation assaysErythroid progenitorsMarrow environmentHematopoietic cellsMessenger RNAPhospho-ERK1/2Systemic iron deficiencyLow ironEpithelial (E)-Cadherin is a Novel Mediator of Platelet Aggregation and Clot Stability
Scanlon VM, Teixeira AM, Tyagi T, Zou S, Zhang PX, Booth CJ, Kowalska MA, Bao J, Hwa J, Hayes V, Marks MS, Poncz M, Krause DS. Epithelial (E)-Cadherin is a Novel Mediator of Platelet Aggregation and Clot Stability. Thrombosis And Haemostasis 2019, 119: 744-757. PMID: 30861547, PMCID: PMC6599679, DOI: 10.1055/s-0039-1679908.Peer-Reviewed Original ResearchConceptsConditional knockout miceKnockout micePlatelet aggregationE-cadherinClot stabilityClot stabilizationSynthase kinase 3β activationAntibody-mediated platelet depletionVivo injury modelsNull plateletsPlatelet productionWild-type miceTail bleeding timeAkt/GSK3βMurine platelet aggregationKnockout mouse modelPlatelet dysfunctionFibrin depositionInjury modelPlatelet depletionPrimary human plateletsBleeding timeMouse modelPlatelet numberE-cadherin antibody
2015
Role of Cbl-PI3K Interaction during Skeletal Remodeling in a Murine Model of Bone Repair
Scanlon V, Soung do Y, Adapala NS, Morgan E, Hansen MF, Drissi H, Sanjay A. Role of Cbl-PI3K Interaction during Skeletal Remodeling in a Murine Model of Bone Repair. PLOS ONE 2015, 10: e0138194. PMID: 26393915, PMCID: PMC4578922, DOI: 10.1371/journal.pone.0138194.Peer-Reviewed Original ResearchConceptsCbl-PI3K interactionHistomorphometric analysisFracture callusBone formationMid-diaphyseal femoral fracturePI3KNormal bone homeostasisBony callus formationSoft callus formationHealing femursFemoral fracturesWT miceOsteoclast numberBone resorptionTomography scanMurine modelOsteoblast surfaceFracture healingBone homeostasisBone volumeBone repairSkeletal remodelingMiceBiomechanical testingHigh expression
2014
Loss of Cbl–PI3K interaction in mice prevents significant bone loss following ovariectomy
Adapala NS, Holland D, Scanlon V, Barbe MF, Langdon WY, Tsygankov AY, Lorenzo JA, Sanjay A. Loss of Cbl–PI3K interaction in mice prevents significant bone loss following ovariectomy. Bone 2014, 67: 1-9. PMID: 24994594, PMCID: PMC4149851, DOI: 10.1016/j.bone.2014.06.013.Peer-Reviewed Original ResearchConceptsCbl-PI3K interactionSignificant bone lossBone lossSerum levelsBone resorptionBone remodelingPro-collagen type 1Bone resorbing activityDefective bone resorptionC-terminal telopeptideP1NP levelsSerum CTXResorbing activityL2 vertebraBone volumeOvariectomyTyrosine 737Mice resultsType 1Lumbar vertebraeMiceBone formationOsteoclast migrationBone developmentResorption