2017
Conformational dynamics of a neurotransmitter:sodium symporter in a lipid bilayer
Adhikary S, Deredge DJ, Nagarajan A, Forrest LR, Wintrode PL, Singh SK. Conformational dynamics of a neurotransmitter:sodium symporter in a lipid bilayer. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e1786-e1795. PMID: 28223522, PMCID: PMC5347597, DOI: 10.1073/pnas.1613293114.Peer-Reviewed Original ResearchConceptsSodium symportersMembrane proteinsMammalian membrane proteinsConformational mechanismIntegral membrane proteinsPhospholipid bilayer nanodiscsSolution spectroscopyDetergent-solubilized stateExtracellular loop 2Site-specific labelingSmall molecule neurotransmittersBilayer nanodiscsHydrogen-deuterium exchangeX-ray crystallographyCysteine accessibilityConformational dynamicsMembrane mimicsMolecular dynamics simulationsLoop 2Endogenous cysteineHelices 1ALeuTLipid bilayersProteinSymporter
2005
Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters
Yamashita A, Singh SK, Kawate T, Jin Y, Gouaux E. Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters. Nature 2005, 437: 215-223. PMID: 16041361, DOI: 10.1038/nature03978.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacteriaBacterial ProteinsBinding SitesBiological TransportChloridesCrystallography, X-RayHydrophobic and Hydrophilic InteractionsLeucineMembrane Transport ProteinsModels, MolecularMolecular Sequence DataNeurotransmitter AgentsSequence AlignmentSodiumStructure-Activity RelationshipWaterConceptsBacterial homologueProtein coreDependent neurotransmitter transportersTransmembrane helicesTransmembrane segmentsAquifex aeolicusUptake of neurotransmittersSubstrate bindingNeurotransmitter transportersMain-chain atomsMembrane bilayerDependent transportersElectrochemical gradientIon binding siteTransportersHelix dipole