2008
Prostate expression of endothelins and their receptors in rat growth
Wada Y, Takahashi W, Latifpour J, Weiss RM, Matsumoto K, Matsui K, Yamada, Imamura T. Prostate expression of endothelins and their receptors in rat growth. Reproduction Fertility And Development 2008, 20: 750-759. PMID: 18842177, DOI: 10.1071/rd08052.Peer-Reviewed Original ResearchConceptsET-1Receptors ETAProstate growthProstate lobesET-3Role of ETAVentral prostate lobeReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionMultiplex reverse transcription-polymerase chain reactionMRNA expression levelsProstate expressionEndothelinMitogenic effectProstate cellsMRNA expressionMembrane particulatesHigh expressionMonthsWeeksEnzyme 1Chain reactionExpression levelsRat growthRats
2004
Age-Related Changes in the Properties of the Endothelin Receptor System at Protein and mRNA Levels in the Rat Vas Deferens
Yono M, Latifpour J, Takahashi W, Pouresmail M, Afiatpour P, Weiss RM. Age-Related Changes in the Properties of the Endothelin Receptor System at Protein and mRNA Levels in the Rat Vas Deferens. Journal Of Receptors And Signal Transduction 2004, 24: 53-66. PMID: 15344879, DOI: 10.1081/rrs-120034106.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAspartic Acid EndopeptidasesDNA PrimersDown-RegulationEndothelin-1Endothelin-3Endothelin-Converting EnzymesKineticsMaleMetalloendopeptidasesPolymerase Chain ReactionProtein BindingRatsRats, Inbred F344Receptor, Endothelin AReceptor, Endothelin BReceptors, EndothelinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerVas DeferensConceptsEndothelin receptor systemET receptor systemAge-related changesWeek old ratsET receptorsMonth old ratsOld ratsET-1Receptor systemVas deferensReceptor subtypesRat vasET-3MRNA levelsAge groupsFunctional ET receptorsTotal ET receptorsReceptor subtype mRNAsReal-time reverse transcription-polymerase chain reactionRadioligand receptor bindingReverse transcription-polymerase chain reactionPredominant mRNA expressionRat vas deferensTranscription-polymerase chain reactionRadioligand receptor binding assays
2003
Developmental changes in the functional, biochemical and molecular properties of rat bladder endothelin receptors
Afiatpour P, Latifpour J, Takahashi W, Yono M, Foster HE, Ikeda K, Pouresmail M, Weiss RM. Developmental changes in the functional, biochemical and molecular properties of rat bladder endothelin receptors. Naunyn-Schmiedeberg's Archives Of Pharmacology 2003, 367: 462-472. PMID: 12700883, DOI: 10.1007/s00210-003-0715-6.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAutoradiographyBinding SitesEndothelin-1Gene ExpressionIodine RadioisotopesMuscle ContractionMuscle, SmoothRadioligand AssayRatsRats, Inbred F344Receptor, Endothelin AReceptor, Endothelin BReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTime FactorsUrinary BladderConceptsETA receptor subtypeSmooth muscle layerReceptor subtypesRat detrusorEndothelin receptorsET receptorsET-1Muscle layerSelective ETA receptor antagonistET receptor systemLarger contractile responsesTotal ET receptorsReceptor subtype mRNAsETA receptor antagonistETB receptor subtypesReal-time reverse transcription-polymerase chain reactionMuscle bath techniqueBladder smooth muscleReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionAge-dependent decreaseAge-dependent alterationsReal-time RT-PCRContractile responseSubtype mRNAs
2002
Prostaglandin E2 Production and Cyclooxygenase-2 Induction in Human Urinary Tract Infections and Bladder Cancer
Wheeler MA, Hausladen DA, Yoon JH, Weiss RM. Prostaglandin E2 Production and Cyclooxygenase-2 Induction in Human Urinary Tract Infections and Bladder Cancer. Journal Of Urology 2002, 168: 1568-1573. PMID: 12352459, DOI: 10.1016/s0022-5347(05)64522-3.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBCG VaccineCyclooxygenase 1Cyclooxygenase 2DinoprostoneEnzyme InductionFemaleGene Expression Regulation, EnzymologicHumansIsoenzymesMaleMembrane ProteinsMiddle AgedProstaglandin-Endoperoxide SynthasesRNA, MessengerUreterUrinary BladderUrinary Bladder NeoplasmsUrinary Tract InfectionsUrogenital NeoplasmsUrotheliumConceptsUrinary prostaglandin E2 levelsUrinary tract infectionProstaglandin E2 levelsUrinary prostaglandin E2Tract infectionsBladder cancerProstaglandin E2 productionBCG treatmentCOX-2 mRNAUrogenital cancersE2 levelsUrine particulatesBladder muscleInflammatory processProstaglandin E2E2 productionBacillus Calmette-Guerin treatmentCOX-2 protein levelsCOX-2 protein expressionHuman urinary tract infectionsUrinary prostaglandin levelsCOX-2 expressionHuman bladder muscleBladder cancer casesCyclooxygenase-2 inductionQuantification of endothelins, their receptors, and endothelin-converting enzyme mRNA in rat genitourinary tract using real-time RT-PCR
Yono M, Takahashi W, Pouresmail M, Johnson DR, Foster HE, Weiss RM, Latifpour J. Quantification of endothelins, their receptors, and endothelin-converting enzyme mRNA in rat genitourinary tract using real-time RT-PCR. Journal Of Pharmacological And Toxicological Methods 2002, 48: 87-95. PMID: 14565565, DOI: 10.1016/s1056-8719(03)00022-4.Peer-Reviewed Original ResearchConceptsRat genitourinary tractGenitourinary tractET-1MRNA expressionET-3Receptor subtype mRNA expressionReceptor subtype mRNAsETA receptor subtypeReal-time reverse transcription-polymerase chain reactionSubtype mRNA expressionReverse transcription-polymerase chain reactionReceptor-mediated mechanismTranscription-polymerase chain reactionReal-time RT-PCRSubtype mRNAsPolymerase chain reactionReceptor subtypesRat bladderReal-time PCRDorsolateral prostateReceptor mRNAVentral prostateECE-1Computer-assisted programVas deferensThe effect of castration on endothelins, their receptors and endothelin converting enzyme in rat prostate
Takahashi W, Afiatpour P, Foster H, Ikeda K, Wada Y, Weiss RM, Latifpour J. The effect of castration on endothelins, their receptors and endothelin converting enzyme in rat prostate. Naunyn-Schmiedeberg's Archives Of Pharmacology 2002, 366: 166-176. PMID: 12122504, DOI: 10.1007/s00210-002-0575-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAspartic Acid EndopeptidasesAutoradiographyDiabetes Mellitus, ExperimentalDNA PrimersEndothelin-1Endothelin-3Endothelin-Converting EnzymesGene ExpressionMaleMetalloendopeptidasesMicroscopyOrchiectomyOrgan SizeProstateProtein BindingRadioligand AssayRatsRats, Sprague-DawleyReceptor, Endothelin AReceptor, Endothelin BReceptors, EndothelinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerConceptsReceptor subtype mRNAsET receptor subtypesSerum testosterone levelsET receptorsRat prostateEffects of castrationReceptor subtypesSubtype mRNAsDorsolateral regionTestosterone levelsET-1Three-month-old SpraguePharmacological propertiesDiabetes-induced reductionET receptor systemET-3 mRNAETB receptor subtypesPost-operative dayReverse transcription-polymerase chain reactionVentral regionECE-1 mRNATranscription-polymerase chain reactionLight microscopy autoradiographyEnzyme-1 mRNAMultiplex reverse transcription-polymerase chain reaction
2001
Cyclooxygenase-2 protein and prostaglandin E2 production are up-regulated in a rat bladder inflammation model
Wheeler M, Yoon J, Olsson L, Weiss R. Cyclooxygenase-2 protein and prostaglandin E2 production are up-regulated in a rat bladder inflammation model. European Journal Of Pharmacology 2001, 417: 239-248. PMID: 11334856, DOI: 10.1016/s0014-2999(01)00911-6.Peer-Reviewed Original ResearchAdministration, IntravesicalAnimalsBlotting, WesternCyclooxygenase 1Cyclooxygenase 2Cyclooxygenase 2 InhibitorsCyclooxygenase InhibitorsDinoprostoneFemaleInflammationInjections, IntraperitonealIsoenzymesLipopolysaccharidesMembrane ProteinsNiflumic AcidNitratesNitric OxideNitric Oxide DonorsNitric Oxide SynthaseOxidantsPenicillamineProstaglandin-Endoperoxide SynthasesProtaminesRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRNA, MessengerUp-RegulationUrinary BladderRegulatory effect of experimental diabetes on the expression of endothelin receptor subtypes and their gene transcripts in the rat adrenal gland
Ikeda K, Wada Y, Sanematsu H, Foster HE, Shin D, Weiss RM, Latifpour J. Regulatory effect of experimental diabetes on the expression of endothelin receptor subtypes and their gene transcripts in the rat adrenal gland. Journal Of Endocrinology 2001, 168: 163-175. PMID: 11139780, DOI: 10.1677/joe.0.1680163.Peer-Reviewed Original ResearchConceptsEndothelin-converting enzyme-1Rat adrenal glandET receptor subtypesInduction of diabetesAdrenal glandReceptor subtypesETB receptorsExperimental diabetesAge-matched control ratsECE-1 mRNA levelsET-3 mRNAPredominant receptor subtypeDiabetes-induced changesEndothelin receptor subtypesInsulin-treated diabeticsSecretion of steroidsReceptor subtypes ETARadioligand receptor bindingAdrenal gland sizeET-1 mRNAExpression levelsRegulatory effectsVascular toneET receptorsEndothelin action
2000
EXPERIMENTAL DIABETES-INDUCED REGRESSION OF THE RAT PROSTATE IS ASSOCIATED WITH AN INCREASED EXPRESSION OF TRANSFORMING GROWTH FACTOR-β
IKEDA K, WADA Y, FOSTER H, WANG Z, WEISS R, LATIFPOUR J. EXPERIMENTAL DIABETES-INDUCED REGRESSION OF THE RAT PROSTATE IS ASSOCIATED WITH AN INCREASED EXPRESSION OF TRANSFORMING GROWTH FACTOR-β. Journal Of Urology 2000, 164: 180-185. PMID: 10840456, DOI: 10.1016/s0022-5347(05)67491-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDiabetes Mellitus, ExperimentalMaleProstateRatsRats, Sprague-DawleyRNA, MessengerTransforming Growth Factor betaConceptsSerum testosterone levelsDiabetic rat prostateTestosterone levelsRat prostateProstatic weightProtein levelsStromal cellsAge-matched control ratsGrowth factorInsulin-treated diabeticsInduction of diabetesProstatic stromal cellsSemi-quantitative Western blottingTGF-beta expressionTGF-beta2 expressionTransforming Growth FactorAndrogen-dependent processesExperimental diabetesMultiplex RT-PCRControl ratsImmunohistochemical studyTGF-beta2TGF-beta1Prostate glandProstateExpression of endothelin receptor subtypes and their messenger RNAs in diabetic rat prostate: Effect of insulin treatment
Saito M, Wado Y, Ikeda K, Wang Z, Foster H, Smith S, Weiss R, Latifpour J. Expression of endothelin receptor subtypes and their messenger RNAs in diabetic rat prostate: Effect of insulin treatment. Molecular And Cellular Biochemistry 2000, 210: 1-11. PMID: 10976752, DOI: 10.1023/a:1007041909477.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutoradiographyBlood GlucoseBody WeightDiabetes Mellitus, ExperimentalEndothelin Receptor AntagonistsEndothelin-1InsulinIodine RadioisotopesMaleOrgan SizeProstateProtein IsoformsRadioligand AssayRandom AllocationRatsRats, Sprague-DawleyReceptor, Endothelin AReceptor, Endothelin BReceptors, EndothelinReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTestosteroneUp-RegulationConceptsInduction of diabetesET receptorsReverse transcription-polymerase chain reactionRat prostateInsulin treatmentDiabetic animalsReceptor subtypesDiabetes-induced upregulationET receptor densityEndothelin receptor subtypesET receptor subtypesSTZ-injected ratsSerum testosterone levelsRadioligand receptor bindingDiabetic rat prostateTranscription-polymerase chain reactionDiabetic ratsETB receptorsSmaller prostatesEndothelin receptorsProstatic stromaTestosterone levelsReceptor densityPharmacological profileAutoradiographic studyGene expression, localization, and pharmacological characterization of endothelin receptors in diabetic rat bladder dome
Saito M, Wada Y, Ikeda K, Wang Z, Smith S, Foster H, Nishi K, Weiss R, Latifpour J. Gene expression, localization, and pharmacological characterization of endothelin receptors in diabetic rat bladder dome. European Journal Of Pharmacology 2000, 387: 253-263. PMID: 10650170, DOI: 10.1016/s0014-2999(99)00753-0.Peer-Reviewed Original ResearchConceptsRat bladder domeEndothelin receptorsBladder domeInsulin treatmentReceptor subtypesFunctional endothelin receptorsMammalian urinary tractEndothelin receptor subtypesInduction of diabetesAge-matched controlsReceptor alterationsExperimental diabetesUrinary tractPharmacological characterizationPharmacological profileMuscular layerMRNA upregulationAutoradiographic studyTranscription-polymerase chain reaction dataReceptorsExpression levelsDiabetesTissue componentsSubtypesTreatment
1998
Bladder instillation and intraperitoneal injection of Escherichia coli lipopolysaccharide up-regulate cytokines and iNOS in rat urinary bladder.
Olsson LE, Wheeler MA, Sessa WC, Weiss RM. Bladder instillation and intraperitoneal injection of Escherichia coli lipopolysaccharide up-regulate cytokines and iNOS in rat urinary bladder. Journal Of Pharmacology And Experimental Therapeutics 1998, 284: 1203-8. PMID: 9495884.Peer-Reviewed Original ResearchConceptsInducible nitric oxide synthaseUrinary tract infectionIntravesical instillationLocalized inflammatory responseInflammatory responseRat bladderSystemic bacterial lipopolysaccharideInstillation of lipopolysaccharideRat urinary bladderNitric oxide synthaseIL-6 mRNATract infectionsBladder instillationIL-10Urinary nitrateRegulate cytokinesINOS activityIntraperitoneal injectionNOS activityOxide synthaseUrinary bladderImportance of lipopolysaccharideINOS proteinInstillationCGMP levels
1997
Muscarinic Receptor Subtypes and Receptor‐Coupled Phosphatidylinositol Hydrolysis in Rat Bladder Smooth Muscle
Mimata H, Nomura Y, Emoto A, Latifpour J, Wheeler M, Weiss R. Muscarinic Receptor Subtypes and Receptor‐Coupled Phosphatidylinositol Hydrolysis in Rat Bladder Smooth Muscle. International Journal Of Urology 1997, 4: 591-596. PMID: 9477190, DOI: 10.1111/j.1442-2042.1997.tb00315.x.Peer-Reviewed Original ResearchConceptsRat bladder smooth muscleBladder smooth muscleMuscarinic receptor subtypesSmooth muscleM3 receptorsReceptor subtypesPI hydrolysisCarbachol stimulationBladder smooth muscle contractionPhosphatidylinositol hydrolysisN-methylpiperidine methiodideRat urinary bladderSubtype-specific antagonistsSmooth muscle contractionProduction of IP3Expression of mRNAContractile responseUrinary bladderSpecific antagonistMuscle contractionBasal levelsInhibitory effectSubtypesReceptorsMuscleBacterial infection induces nitric oxide synthase in human neutrophils.
Wheeler MA, Smith SD, García-Cardeña G, Nathan CF, Weiss RM, Sessa WC. Bacterial infection induces nitric oxide synthase in human neutrophils. Journal Of Clinical Investigation 1997, 99: 110-116. PMID: 9011564, PMCID: PMC507774, DOI: 10.1172/jci119121.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAnti-Bacterial AgentsArginineBacterial InfectionsBlotting, WesternCanavanineCell MembraneCitrullineFemaleGuanidinesHumansImmunohistochemistryIsoenzymesLeukocyte Common AntigensMaleMiddle AgedNeutrophilsNG-Nitroarginine Methyl EsterNitric Oxide SynthaseNitroarginineOmega-N-MethylarginineOrnithinePolymerase Chain ReactionRNA, MessengerSulfonamidesThioureaTrifluoperazineUrinary Tract InfectionsConceptsInducible nitric oxide synthaseUrinary tract infectionNitric oxide synthaseNitric oxide synthase activityTract infectionsOxide synthase activityOxide synthaseInducible nitric oxide synthase activityNitric oxide synthase proteinNitric oxide synthase isoformsInduces nitric oxide synthaseMajor nitric oxide synthase isoformHuman inflammatory cellsHuman inducible nitric oxide synthaseExtended elevationInflammatory cellsProinflammatory functionsAntibiotic treatmentInflammatory processSynthase activityImmunocytochemical evidenceHuman neutrophilsInfectionCalcium independentSynthase isoforms