2022
Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells
Kantrong N, Chang AM, Bamashmous S, Hajjar AM, Bucala RJ, Darveau RP. Macrophage migration inhibitory factor regulates specific innate immune sensor responses in gingival epithelial cells. The Journal Of Periodontology 2022, 93: 1940-1950. PMID: 35100435, DOI: 10.1002/jper.21-0598.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorGingival epithelial cellsIL-8/CXCL8Migration inhibitory factorGingival tissuesJunctional epitheliumGingival epitheliumRole of MIFInhibitory factorMIF KO miceEpithelial cellsExpression of TLR4Healthy gingival tissuesToll-like receptorsInterleukin-1 receptorRepertoire of receptorsOral healthNeutrophil chemoattractantIL-1R1IL-1R2Neutrophil regulationKO micePeriodontal tissuesIL-1ROral homeostasis
2021
Hsp90-stabilized MIF supports tumor progression via macrophage recruitment and angiogenesis in colorectal cancer
Klemke L, De Oliveira T, Witt D, Winkler N, Bohnenberger H, Bucala R, Conradi LC, Schulz-Heddergott R. Hsp90-stabilized MIF supports tumor progression via macrophage recruitment and angiogenesis in colorectal cancer. Cell Death & Disease 2021, 12: 155. PMID: 33542244, PMCID: PMC7862487, DOI: 10.1038/s41419-021-03426-z.Peer-Reviewed Original ResearchMeSH KeywordsAngiogenic ProteinsAnimalsAntigens, Differentiation, B-LymphocyteAntineoplastic AgentsColitis-Associated NeoplasmsDisease Models, AnimalFemaleHCT116 CellsHEK293 CellsHistocompatibility Antigens Class IIHSP90 Heat-Shock ProteinsHumansInflammation MediatorsIntramolecular OxidoreductasesMacrophage Migration-Inhibitory FactorsMaleMice, Inbred C57BLMice, KnockoutNeovascularization, PathologicOrganoidsProtein StabilitySignal TransductionTumor BurdenTumor-Associated MacrophagesConceptsMacrophage migration inhibitory factorMIF levelsMacrophage recruitmentAction of MIFColitis-associated colorectal cancer (CAC) mouse modelTumor growthTumor progressionFunction of MIFColorectal cancer mouse modelHigher MIF levelsHost inflammatory pathwaysTumor-specific functionsEpithelial cellsShorter overall survivalCRC tumor progressionClinical correlation studiesMigration inhibitory factorCRC tumor growthCancer mouse modelWild-type organoidsTumor epithelial cellsHSP90 inhibitor treatmentCD74 expressionOverall survivalCRC patients
2019
2598. Macrophage Migration Inhibitory Factor May Contribute to Disseminated Coccidioidomycosis Susceptibility
Odio C, Leng L, Siu E, Piecychna M, Galgiani J, Holland S, Bucala R. 2598. Macrophage Migration Inhibitory Factor May Contribute to Disseminated Coccidioidomycosis Susceptibility. Open Forum Infectious Diseases 2019, 6: s903-s903. PMCID: PMC6810323, DOI: 10.1093/ofid/ofz360.2276.Peer-Reviewed Original ResearchMacrophage migration inhibitory factorPlasma MIF levelsHigh MIF expressionMigration inhibitory factorHuman lung epithelial cellsMIF expressionDisseminated coccidioidomycosisHealthy controlsLung epithelial cellsMIF levelsHigh-expression MIF allelesInhibitory factorEpithelial cellsFisher's exact testCATT5-8MIF polymorphismsSelf-identified African AmericansInflammatory cytokinesMIF allelesGranulomatous diseaseLow-expression allelePromoter polymorphismCoccidioidomycosis patientsPatientsExact test
2001
Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa
Eickhoff R, Wilhelm B, Renneberg H, Wennemuth G, Bacher M, Linder D, Bucala R, Seitz J, Meinhardt A. Purification and Characterization of Macrophage Migration Inhibitory Factor as a Secretory Protein from Rat Epididymis: Evidences for Alternative Release and Transfer to Spermatozoa. Molecular Medicine 2001, 7: 27-35. PMID: 11474125, PMCID: PMC1949991, DOI: 10.1007/bf03401836.Peer-Reviewed Original ResearchConceptsOuter dense fibersSequence analysisDense fibersSecretion modeCDNA fragmentsN-terminal signal sequenceEndoplasmic reticulumMacrophage migration inhibitory factorEpithelial cellsCytokine macrophage migration inhibitory factorN-terminal amino acid sequence analysisAmino acid sequence analysisApical cell surfaceAcid sequence analysisEpididymal epithelial cellsRat epididymisSignal sequenceCDNA sequenceWestern blot experimentsPlasma membraneMigration inhibitory factorSecretory proteinsCytoskeletal elementsExpression patternsN-terminus
2000
Expression of macrophage migration inhibitory factor in human glomerulonephritis
Lan H, Yang N, Nikolic-Paterson D, Yu X, Mu W, Isbel N, Metz C, Bucala R, Atkins R. Expression of macrophage migration inhibitory factor in human glomerulonephritis. Kidney International 2000, 57: 499-509. PMID: 10652026, DOI: 10.1046/j.1523-1755.2000.00869.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overBiopsyCohort StudiesEpithelial CellsFemaleGene ExpressionGlomerulonephritis, MembranoproliferativeGlomerulonephritis, MembranousHumansIn Situ HybridizationKidney GlomerulusMacrophage Migration-Inhibitory FactorsMacrophagesMaleMiddle AgedReference ValuesRNA, MessengerT-LymphocytesConceptsMacrophage migration inhibitory factorMIF expressionMigration inhibitory factorFocal segmental glomerulosclerosisHuman glomerulonephritisProliferative formsMIF mRNAPathogenic roleExperimental glomerulonephritisInhibitory factorProgressive formRenal MIF expressionRenal function impairmentT cell accumulationT-cell infiltratesEpithelial cellsMinimal change diseaseFocal segmental lesionsGlomerular endothelial cellsTubular epithelial cellsNormal human kidneyAttractive therapeutic targetCreatinine clearanceGlomerular epithelial cellsLupus nephritis