2024
An atlas of human vector-borne microbe interactions reveals pathogenicity mechanisms
Hart T, Sonnert N, Tang X, Chaurasia R, Allen P, Hunt J, Read C, Johnson E, Arora G, Dai Y, Cui Y, Chuang Y, Yu Q, Rahman M, Mendes M, Rolandelli A, Singh P, Tripathi A, Ben Mamoun C, Caimano M, Radolf J, Lin Y, Fingerle V, Margos G, Pal U, Johnson R, Pedra J, Azad A, Salje J, Dimopoulos G, Vinetz J, Carlyon J, Palm N, Fikrig E, Ring A. An atlas of human vector-borne microbe interactions reveals pathogenicity mechanisms. Cell 2024, 187: 4113-4127.e13. PMID: 38876107, DOI: 10.1016/j.cell.2024.05.023.Peer-Reviewed Original ResearchCell invasionHost-microbe interactionsArthropod-borne pathogensHost sensingMicrobe interactionsTranscriptional regulationLyme disease spirocheteMicrobial interactionsExtracellular proteinsMicrobial pathogenesisEpidermal growth factorTissue colonizationEnvironmental cuesBacterial selectivityIntracellular pathogensPutative interactionsNext-generation therapeuticsPathogensFunctional investigationsInteractomeVector-borne diseasesImmune evasionPathogenic mechanismsStrainUnmet medical need
2022
Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures
Johnson RM, Asashima H, Mohanty S, Shaw AC. Combining Cellular Immunology With RNAseq to Identify Novel Chlamydia T-Cell Subset Signatures. The Journal Of Infectious Diseases 2022, 225: 2033-2042. PMID: 35172331, PMCID: PMC9159333, DOI: 10.1093/infdis/jiac051.Peer-Reviewed Original ResearchConceptsProtective T cell clonesAntibacterial effector mechanismsT cells residentB cell helpT cell clonesCytokine polarizationImmune miceIL-10Protective immunityVaccine trialsIL-13Surrogate biomarkerEffector mechanismsGenital tractT cellsVaccine candidatesChlamydia trachomatisCells residentHelper functionCellular immunologyMouse studiesHuman investigationsReproductive tractGranzyme A.Investigational data
2020
Covid-19: Should doctors recommend treatments and vaccines when full data are not publicly available?
Johnson RM, Doshi P, Healy D. Covid-19: Should doctors recommend treatments and vaccines when full data are not publicly available? BMJ (Clinical Research Ed.) 2020, 370: m3260. PMID: 32839164, DOI: 10.1136/bmj.m3260.Commentaries, Editorials and LettersDexamethasone in the management of covid -19
Johnson RM, Vinetz JM. Dexamethasone in the management of covid -19. The BMJ 2020, 370: m2648. PMID: 32620554, DOI: 10.1136/bmj.m2648.Commentaries, Editorials and LettersComparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine
Yu H, Karunakaran KP, Jiang X, Chan Q, Rose C, Foster LJ, Johnson RM, Brunham RC. Comparison of Chlamydia outer membrane complex to recombinant outer membrane proteins as vaccine. Vaccine 2020, 38: 3280-3291. PMID: 32151463, DOI: 10.1016/j.vaccine.2020.02.059.Peer-Reviewed Original ResearchConceptsChlamydial outer membrane complexRecombinant outer membrane proteinsImmune responseT-cell vaccine candidatesElementary bodiesReactive immune responsesC. muridarum elementary bodiesGenital infectionMajor outer membrane proteinTubal pathologyC. trachomatis serovarsVaccine efficacyOuter membrane proteinsSuperior efficacyVaccine candidatesBroad immunogenicityC. trachomatisVaccineAntibodiesSuperior protectionMultiple outer membrane proteinsOuter membrane complexPathologyEfficacyPolymorphic membrane proteinsA Class II-Restricted CD8γ13 T-Cell Clone Protects During Chlamydia muridarum Genital Tract Infection
Johnson RM, Olivares-Strank N, Peng G. A Class II-Restricted CD8γ13 T-Cell Clone Protects During Chlamydia muridarum Genital Tract Infection. The Journal Of Infectious Diseases 2020, 221: 1895-1906. PMID: 31899500, PMCID: PMC7213565, DOI: 10.1093/infdis/jiz685.Peer-Reviewed Original ResearchConceptsGenital tract infectionCD8 T cellsT cell clonesChlamydia muridarum Genital Tract InfectionMHC class IIT cellsTract infectionsClass IIClass IAntigen-specific CD8 T cellsChlamydia genital tract infectionCD8 T cell clonesCD4 T cell numbersMajor histocompatibility complex class IAdoptive transfer studiesT cell numbersT cell responsesHistocompatibility complex class IComplex class IBacterial clearanceIL-13IL-5ImmunopathologyIntracellular pathogensInfectionAdaptive Immunity to Chlamydia trachomatis Infection
Adaptive Immunity to Chlamydia trachomatis Infection T.B. Poston, T. Darville, and R.M. Johnson, (2020), “Adaptive Immunity to Chlamydia trachomatis Infection”, In Chlamydia Biology: From Genome to Disease, Catherine Suetterlin, Johannes Hegemann, Ming Tan Eds. (Caister Academic Press, U.K.)Books
2019
Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development
Karunakaran KP, Yu H, Jiang X, Chan QWT, Foster LJ, Johnson RM, Brunham RC. Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development. The Journal Of Infectious Diseases 2019, 221: 841-850. PMID: 31599954, PMCID: PMC7457330, DOI: 10.1093/infdis/jiz522.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialBacterial VaccinesCD4-Positive T-LymphocytesCell LineChlamydia InfectionsChlamydia muridarumChlamydia trachomatisDendritic CellsEpithelial CellsEpitopes, T-LymphocyteFemaleHeLa CellsHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHost-Pathogen InteractionsHumansMiceMice, Inbred C57BLPeptidesConceptsCD4 T cellsDendritic cellsT cellsEpithelial cellsProtective immunityEffector phaseClass IChlamydia-specific CD4 T cellsPathogen-specific T cellsClass IIMajor histocompatibility complex (MHC) class II moleculesChlamydia vaccine developmentClearance of ChlamydiaClass II epitopesClass II moleculesMHC class IMucosal epithelial cellsInfected epithelial cellsImmune miceIntracellular bacterial pathogenChlamydia vaccineC trachomatisEpithelial cell linePresent epitopesChlamydia trachomatis
2018
B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters
Johnson RM, Yu H, Strank N, Karunakaran K, Zhu Y, Brunham RC. B Cell Presentation of Chlamydia Antigen Selects Out Protective CD4γ13 T Cells: Implications for Genital Tract Tissue-Resident Memory Lymphocyte Clusters. Infection And Immunity 2018, 86: 10.1128/iai.00614-17. PMID: 29158429, PMCID: PMC5778355, DOI: 10.1128/iai.00614-17.Peer-Reviewed Original ResearchConceptsMemory lymphocyte clustersPeripheral blood mononuclear cellsT cell subsetsAntigen-presenting cellsT cell clonesB cell populationsT cellsLymphocyte clustersCell subsetsB cellsTissue-resident memory T cellsCD4 T-cell clonesCD4 T cell subsetsHuman peripheral blood mononuclear cellsCell clonesMemory T cellsBlood mononuclear cellsIL-13 responsesImmune B cellsB cell presentationCell populationsAdoptive transferImmune miceIntracellular bacterial pathogenChlamydia antigen
2016
Pediatric Kawasaki Disease and Adult Human Immunodeficiency Virus Kawasaki-Like Syndrome Are Likely the Same Malady
Johnson RM, Bergmann KR, Manaloor JJ, Yu X, Slaven JE, Kharbanda AB. Pediatric Kawasaki Disease and Adult Human Immunodeficiency Virus Kawasaki-Like Syndrome Are Likely the Same Malady. Open Forum Infectious Diseases 2016, 3: ofw160. PMID: 27704015, PMCID: PMC5047405, DOI: 10.1093/ofid/ofw160.Peer-Reviewed Original ResearchKawasaki-like syndromeHuman immunodeficiency virusEnzyme-linked immunosorbent assayMultiplex enzyme-linked immunosorbent assayPediatric Kawasaki diseaseKawasaki diseaseAcute phase reactantsInflammatory signatureFebrile controlsKD subjectsAsymptomatic human immunodeficiency virusElevated acute phase reactantsCommon etiologic agentTumor necrosis factorSimilar physical findingsAntiretroviral therapyConvalescent phaseFebrile illnessCytokine milieuIg therapyInflamed arteriesCommon etiologyImmunodeficiency virusPhysical findingsTherapeutic responseTissue-Resident T Cells as the Central Paradigm of Chlamydia Immunity
Johnson RM, Brunham RC. Tissue-Resident T Cells as the Central Paradigm of Chlamydia Immunity. Infection And Immunity 2016, 84: 868-873. PMID: 26787715, PMCID: PMC4807466, DOI: 10.1128/iai.01378-15.Peer-Reviewed Original ResearchConceptsT cellsTissue-resident memory T cellsTissue-resident T cellsMemory T cellsChlamydia immunityCytokine polarizationTrachoma vaccineChlamydia vaccineVaccine efficacyPathogenesis dataPathogenesis investigationPreclinical pipelineImmunity studiesHuman subjectsVaccineImmune networkCellsMiceImmunity
2015
Modeling the transcriptome of genital tract epithelial cells and macrophages in healthy mucosa versus mucosa inflamed by Chlamydia muridarum infection
Johnson RM, Kerr MS. Modeling the transcriptome of genital tract epithelial cells and macrophages in healthy mucosa versus mucosa inflamed by Chlamydia muridarum infection. Pathogens And Disease 2015, 73: ftv100. PMID: 26519447, PMCID: PMC4732027, DOI: 10.1093/femspd/ftv100.Peer-Reviewed Original ResearchConceptsEpithelial cellsLineage-specific differencesGene expression microarray technologyExpression microarray technologyBone marrow-derived macrophagesMarrow-derived macrophagesAdaptive immunityReproductive tract epitheliumCell line responsePrincipal cell typesMicroarray technologyIntracellular bacteriaChlamydia-specific T cellsGenital tract epithelial cellsCell typesBacterial replicationChlamydia muridarum infectionMHC class II moleculesEpithelial requirementTract epithelial cellsClass II moleculesInnate defenseHost defenseCoinhibitory ligandsInflamed/
2014
An atypical CD8 T‐cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin‐13
Johnson RM, Kerr MS, Slaven JE. An atypical CD8 T‐cell response to Chlamydia muridarum genital tract infections includes T cells that produce interleukin‐13. Immunology 2014, 142: 248-257. PMID: 24428415, PMCID: PMC4008232, DOI: 10.1111/imm.12248.Peer-Reviewed Original ResearchConceptsGenital tract infectionCD8 T cellsCD8 T cell responsesCD8 T cell clonesT cell responsesTract infectionsT cell clonesT cellsProtective immunityInterleukin-13T helper type 1 cell responsesC. muridarum genital tract infectionChlamydia muridarum Genital Tract InfectionMHC class Ia moleculesCD4 T cellsRole of TNFAntigen-presenting cellsTumor necrosis factorReproductive tract epitheliumClass Ia moleculesCD8 clonesCD8 levelsChlamydia replicationNaive splenocytesIntracellular bacterial pathogen
2013
Correction: Perforin Is Detrimental to Controlling C. muridarum Replication In Vitro, but Not In Vivo
Johnson R, Kerr M, Slaven J. Correction: Perforin Is Detrimental to Controlling C. muridarum Replication In Vitro, but Not In Vivo. PLOS ONE 2013, 8: 10.1371/annotation/b7213da3-498c-43bf-b42c-1f22934e17dd. PMCID: PMC3741034, DOI: 10.1371/annotation/b7213da3-498c-43bf-b42c-1f22934e17dd.Peer-Reviewed Original ResearchPerforin Is Detrimental to Controllinγ C. muridarum Replication In Vitro, but Not In Vivo
Johnson RM, Kerr MS, Slaven JE. Perforin Is Detrimental to Controllinγ C. muridarum Replication In Vitro, but Not In Vivo. PLOS ONE 2013, 8: e63340. PMID: 23691028, PMCID: PMC3653963, DOI: 10.1371/journal.pone.0063340.Peer-Reviewed Original ResearchConceptsGenital tract infectionPerforin knockout miceChlamydia replicationTract infectionsEpithelial cellsKnockout miceC. muridarum genital tract infectionChlamydia muridarum Genital Tract InfectionClearance mechanismsVivo clearance mechanismsCD4 T cellsT cell mechanismsT cell-epithelial cell interactionsT cell degranulationNitric oxide productionBacterial clearanceEpithelial productionCell degranulationT cellsSingle-gene knockout miceWeek 7Oxide productionPerforinNitric oxideInfectionChlamydial Diseases
Johnson R. Chlamydial Diseases. 2013, 469-497. DOI: 10.1007/978-3-642-30144-5_111.ChaptersHost speciesSpecific host speciesTissue tropismBacterial adaptationGenetic codeEnvironmental nichesSpecific nichesAdaptive host defensesHuman diseasesChlamydia speciesSpeciesPathogenic bacteriaChlamydia researchChlamydiaeAbsolute dependenceHost defenseNicheChlamydial diseaseBacteriaHostDefenseTropismHuman illnessPathogensVaccine development
2012
PmpG303-311, a Protective Vaccine Epitope That Elicits Persistent Cellular Immune Responses in Chlamydia muridarum-Immune Mice
Johnson RM, Yu H, Kerr MS, Slaven JE, Karunakaran KP, Brunham RC. PmpG303-311, a Protective Vaccine Epitope That Elicits Persistent Cellular Immune Responses in Chlamydia muridarum-Immune Mice. Infection And Immunity 2012, 80: 2204-2211. PMID: 22431650, PMCID: PMC3370596, DOI: 10.1128/iai.06339-11.Peer-Reviewed Original ResearchConceptsGenital tract infectionAntigen-presenting cellsT cell clonesTract infectionsMonths postinfectionImmune responseChlamydia muridarum Genital Tract InfectionProtective CD4 T cell responsesVaccine developmentPrimary genital tract infectionTh1 T cell clonesCD4 T cell responsesCD4 T-cell clonesSplenic antigen-presenting cellsAntigen-presenting cell populationsCell clonesCD4 T cellsT cell responsesCellular immune responsesProtective immune responsePromising vaccine candidateImmune C57BL/6 miceInfected epithelial cellsIrradiated splenocytesUrogenital chlamydiaPlac8-Dependent and Inducible NO Synthase-Dependent Mechanisms Clear Chlamydia muridarum Infections from the Genital Tract
Johnson RM, Kerr MS, Slaven JE. Plac8-Dependent and Inducible NO Synthase-Dependent Mechanisms Clear Chlamydia muridarum Infections from the Genital Tract. The Journal Of Immunology 2012, 188: 1896-1904. PMID: 22238459, PMCID: PMC3303601, DOI: 10.4049/jimmunol.1102764.Peer-Reviewed Original ResearchConceptsGenital tract infectionCD4 T-cell clonesINOS-independent mechanismCD4 T cellsT cell clonesTract infectionsT cellsC. muridarum genital tract infectionCell clonesChlamydia muridarum infectionInducible NO synthase (iNOS) transcriptionT cell mechanismsT cell subsetsClearance of infectionGenital tract epitheliumT cell degranulationCytokine profileCell subsetsCell degranulationGenital tractClear infectionEffector functionsMouse modelNO productionVaccine development
2010
Chlamydia-Specific CD4 T Cell Clones Control Chlamydia muridarum Replication in Epithelial Cells by Nitric Oxide-Dependent and -Independent Mechanisms
Jayarapu K, Kerr M, Ofner S, Johnson RM. Chlamydia-Specific CD4 T Cell Clones Control Chlamydia muridarum Replication in Epithelial Cells by Nitric Oxide-Dependent and -Independent Mechanisms. The Journal Of Immunology 2010, 185: 6911-6920. PMID: 21037093, PMCID: PMC3073083, DOI: 10.4049/jimmunol.1002596.Peer-Reviewed Original ResearchConceptsCD4 T-cell clonesT cell clonesEpithelial NO productionCD4 T cellsChlamydia replicationCell clonesEpithelial cellsT cellsNO productionReproductive tract epithelial cellsT cell-mediated controlT-cell depletion studiesCell depletion studiesCell-mediated controlHuman reproductive tractT cell degranulationMHC class IIMurine genital tractTract epithelial cellsInfected epithelial cellsEpithelial tumor cell linesIntracellular bacterial pathogenBacterial clearanceCell degranulationGenital tract
2009
Chlamydia muridarum-Specific CD4 T-Cell Clones Recognize Infected Reproductive Tract Epithelial Cells in an Interferon-Dependent Fashion
Jayarapu K, Kerr MS, Katschke A, Johnson RM. Chlamydia muridarum-Specific CD4 T-Cell Clones Recognize Infected Reproductive Tract Epithelial Cells in an Interferon-Dependent Fashion. Infection And Immunity 2009, 77: 4469-4479. PMID: 19667042, PMCID: PMC2747947, DOI: 10.1128/iai.00491-09.Peer-Reviewed Original ResearchConceptsCD4 T-cell clonesT cell clonesReproductive tract epithelial cellsCD4 T cell interactionsT cell activationGenital tract infectionCD4 T cellsTract epithelial cellsT cell interactionsEpithelial cellsTract infectionsMHC-IIT cellsChlamydia muridarum Genital Tract InfectionChlamydia-specific CD4 T cellsMajor histocompatibility complex (MHC) class II moleculesIFN-gamma-induced upregulationCell surface MHC-IIExperimental mouse modelSurface MHC-IIClass II moleculesReproductive tract epitheliumTiming of recognitionFuture vaccine developmentChlamydia replication