2019
Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development
Karunakaran KP, Yu H, Jiang X, Chan QWT, Foster LJ, Johnson RM, Brunham RC. Discordance in the Epithelial Cell-Dendritic Cell Major Histocompatibility Complex Class II Immunoproteome: Implications for Chlamydia Vaccine Development. The Journal Of Infectious Diseases 2019, 221: 841-850. PMID: 31599954, PMCID: PMC7457330, DOI: 10.1093/infdis/jiz522.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, BacterialBacterial VaccinesCD4-Positive T-LymphocytesCell LineChlamydia InfectionsChlamydia muridarumChlamydia trachomatisDendritic CellsEpithelial CellsEpitopes, T-LymphocyteFemaleHeLa CellsHistocompatibility Antigens Class IHistocompatibility Antigens Class IIHost-Pathogen InteractionsHumansMiceMice, Inbred C57BLPeptidesConceptsCD4 T cellsDendritic cellsT cellsEpithelial cellsProtective immunityEffector phaseClass IChlamydia-specific CD4 T cellsPathogen-specific T cellsClass IIMajor histocompatibility complex (MHC) class II moleculesChlamydia vaccine developmentClearance of ChlamydiaClass II epitopesClass II moleculesMHC class IMucosal epithelial cellsInfected epithelial cellsImmune miceIntracellular bacterial pathogenChlamydia vaccineC trachomatisEpithelial cell linePresent epitopesChlamydia trachomatis
2015
Modeling the transcriptome of genital tract epithelial cells and macrophages in healthy mucosa versus mucosa inflamed by Chlamydia muridarum infection
Johnson RM, Kerr MS. Modeling the transcriptome of genital tract epithelial cells and macrophages in healthy mucosa versus mucosa inflamed by Chlamydia muridarum infection. Pathogens And Disease 2015, 73: ftv100. PMID: 26519447, PMCID: PMC4732027, DOI: 10.1093/femspd/ftv100.Peer-Reviewed Original ResearchConceptsEpithelial cellsLineage-specific differencesGene expression microarray technologyExpression microarray technologyBone marrow-derived macrophagesMarrow-derived macrophagesAdaptive immunityReproductive tract epitheliumCell line responsePrincipal cell typesMicroarray technologyIntracellular bacteriaChlamydia-specific T cellsGenital tract epithelial cellsCell typesBacterial replicationChlamydia muridarum infectionMHC class II moleculesEpithelial requirementTract epithelial cellsClass II moleculesInnate defenseHost defenseCoinhibitory ligandsInflamed/
2013
Perforin Is Detrimental to Controllinγ C. muridarum Replication In Vitro, but Not In Vivo
Johnson RM, Kerr MS, Slaven JE. Perforin Is Detrimental to Controllinγ C. muridarum Replication In Vitro, but Not In Vivo. PLOS ONE 2013, 8: e63340. PMID: 23691028, PMCID: PMC3653963, DOI: 10.1371/journal.pone.0063340.Peer-Reviewed Original ResearchConceptsGenital tract infectionPerforin knockout miceChlamydia replicationTract infectionsEpithelial cellsKnockout miceC. muridarum genital tract infectionChlamydia muridarum Genital Tract InfectionClearance mechanismsVivo clearance mechanismsCD4 T cellsT cell mechanismsT cell-epithelial cell interactionsT cell degranulationNitric oxide productionBacterial clearanceEpithelial productionCell degranulationT cellsSingle-gene knockout miceWeek 7Oxide productionPerforinNitric oxideInfection
2010
Chlamydia-Specific CD4 T Cell Clones Control Chlamydia muridarum Replication in Epithelial Cells by Nitric Oxide-Dependent and -Independent Mechanisms
Jayarapu K, Kerr M, Ofner S, Johnson RM. Chlamydia-Specific CD4 T Cell Clones Control Chlamydia muridarum Replication in Epithelial Cells by Nitric Oxide-Dependent and -Independent Mechanisms. The Journal Of Immunology 2010, 185: 6911-6920. PMID: 21037093, PMCID: PMC3073083, DOI: 10.4049/jimmunol.1002596.Peer-Reviewed Original ResearchConceptsCD4 T-cell clonesT cell clonesEpithelial NO productionCD4 T cellsChlamydia replicationCell clonesEpithelial cellsT cellsNO productionReproductive tract epithelial cellsT cell-mediated controlT-cell depletion studiesCell depletion studiesCell-mediated controlHuman reproductive tractT cell degranulationMHC class IIMurine genital tractTract epithelial cellsInfected epithelial cellsEpithelial tumor cell linesIntracellular bacterial pathogenBacterial clearanceCell degranulationGenital tract
2009
Chlamydia muridarum-Specific CD4 T-Cell Clones Recognize Infected Reproductive Tract Epithelial Cells in an Interferon-Dependent Fashion
Jayarapu K, Kerr MS, Katschke A, Johnson RM. Chlamydia muridarum-Specific CD4 T-Cell Clones Recognize Infected Reproductive Tract Epithelial Cells in an Interferon-Dependent Fashion. Infection And Immunity 2009, 77: 4469-4479. PMID: 19667042, PMCID: PMC2747947, DOI: 10.1128/iai.00491-09.Peer-Reviewed Original ResearchConceptsCD4 T-cell clonesT cell clonesReproductive tract epithelial cellsCD4 T cell interactionsT cell activationGenital tract infectionCD4 T cellsTract epithelial cellsT cell interactionsEpithelial cellsTract infectionsMHC-IIT cellsChlamydia muridarum Genital Tract InfectionChlamydia-specific CD4 T cellsMajor histocompatibility complex (MHC) class II moleculesIFN-gamma-induced upregulationCell surface MHC-IIExperimental mouse modelSurface MHC-IIClass II moleculesReproductive tract epitheliumTiming of recognitionFuture vaccine developmentChlamydia replication