1990
Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1988
Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells.
Van Itallie C, Dannies P. Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells. Endocrinology 1988, 2: 332-7. PMID: 2837664, DOI: 10.1210/mend-2-4-332.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DivisionDNADNA, RecombinantElectron Transport Complex IVEnzyme InductionEpidermal Growth FactorEstradiolFemaleInsulinMaleMitochondriaMolecular Sequence DataNucleic Acid HybridizationPituitary NeoplasmsProlactinRatsRats, Inbred StrainsRNA, MessengerTumor Cells, CulturedConceptsCytochrome oxidase II geneCytochrome oxidaseMitochondrial ribonucleic acidMitochondrial genomePituitary tumor cell lineEpidermal growth factorMRNA accumulationNumber of copiesTumor cell linesPituitary tumor cellsCell proliferationMRNACell linesRibonucleic acidMaximal inductionGenesGrowth factorGrowth ratePRL mRNA
1982
Somatic cell hybridization of growth hormone-producing rat pituitary cells and mouse fibroblasts results in extinction of growth hormone expression via a defect in growth hormone RNA production.
Strobl J, Dannies P, Thompson E. Somatic cell hybridization of growth hormone-producing rat pituitary cells and mouse fibroblasts results in extinction of growth hormone expression via a defect in growth hormone RNA production. Journal Of Biological Chemistry 1982, 257: 6588-6594. PMID: 6176584, DOI: 10.1016/s0021-9258(20)65183-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DivisionCell LineDNADNA Restriction EnzymesGliomaGrowth HormoneHybrid CellsL CellsMiceNucleic Acid HybridizationPoly ARatsRNARNA, MessengerTranscription, GeneticConceptsMouse GH geneGH geneHybrid cellsHybrid cell DNAHigh molecular weight precursorNon-pituitary cellsSomatic cell hybridizationMolecular weight precursorMultiple restriction enzymesStructural geneTranscript degradationMature mRNARestriction enzyme analysisIncomplete transcriptsFlanking sequencesParental cell lineTranscriptional blockRestriction enzyme digestionRNA productionSouthern hybridizationCell hybridizationMRNA sequencesFibroblasts resultsGrowth hormone expressionMouse fibroblast cells