2004
Is there structural specificity in the reversible protein aggregates that are stored in secretory granules?
Keeler C, Hodsdon ME, Dannies PS. Is there structural specificity in the reversible protein aggregates that are stored in secretory granules? Journal Of Molecular Neuroscience 2004, 22: 43-49. PMID: 14742909, DOI: 10.1385/jmn:22:1-2:43.Peer-Reviewed Original ResearchConceptsSecretory granule proteinsMembrane proteinsGranule functionGranule proteinsAmino acid residuesSecretory granulesExcess membraneProtein aggregatesSecretory proteinsAcid residuesProteinStructural specificityResiduesSurface motifsGranulesAggregation processMembraneReversible aggregationSpecificityMotifNMR spectroscopyAggregationAggregatesCellsAccumulation
2003
The Tertiary Structure and Backbone Dynamics of Human Prolactin
Keeler C, Dannies PS, Hodsdon ME. The Tertiary Structure and Backbone Dynamics of Human Prolactin. Journal Of Molecular Biology 2003, 328: 1105-1121. PMID: 12729745, DOI: 10.1016/s0022-2836(03)00367-x.Peer-Reviewed Original ResearchConceptsFour-dimensional heteronuclear NMR spectroscopyTertiary structureBackbone dynamicsFour-helical bundleN-terminal loopSecretory granulesHeteronuclear NMR spectroscopyExtrapituitary prolactinNMR relaxation phenomenaThird helixFirst helixSecond helixGolgi complexFunctional bindingHematopoietic cytokinesBundle topologyDiscrete structural differencesFemale reproductive systemHuman prolactinProlactin receptorReversible oligomerizationHelixShort loopsReproductive systemGrowth factor
1995
Biological activity and immunological reactivity of human prolactin mutants
Rhee H, Sun Z, Kim S, Goffin V, Martial J, Dannies P. Biological activity and immunological reactivity of human prolactin mutants. Endocrinology 1995, 136: 4990-4995. PMID: 7588233, DOI: 10.1210/endo.136.11.7588233.Peer-Reviewed Original ResearchConceptsSerine 90Cystine loopBiological activityRat pituitary cell lineHuman PRLPituitary cell lineWild typeCertain fishPRL-3MutantsTryptophan fluorescenceCell linesMutationsFishT mutationCell assayPRL storageImmunological reactivityImmunological propertiesSerineConcentration of ureaActivityConservationSpacial requirementsLoop