2000
Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH
Lee M, Wajnrajch M, Kim S, Plotnick L, Wang J, Gertner J, Leibel R, Dannies P. Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH. Endocrinology 2000, 141: 883-890. PMID: 10698162, DOI: 10.1210/endo.141.3.7380.Peer-Reviewed Original ResearchConceptsWild-type GHSecretory pathway functionNeuroendocrine cell lineGH deficiency type IISuppression of accumulationPathway functionTransient transfectionIntracellular stabilityCHO cellsAutosomal dominant formCell linesDecreased stabilityNormal allelePosttranslational effectGeneral suppressionCoexpressionProtein
1996
KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells
Cui Z, Hidaka H, Dannies P. KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells. Biochimica Et Biophysica Acta 1996, 1310: 343-347. PMID: 8599613, DOI: 10.1016/0167-4889(95)00170-0.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsCalciumCalcium-Calmodulin-Dependent Protein KinasesCells, CulturedEnzyme InhibitorsFemaleIsoquinolinesKineticsPiperazinesPituitary Gland, AnteriorPotassiumPotassium ChlorideProlactinRatsRats, Sprague-DawleyThyrotropin-Releasing HormoneTime FactorsConceptsAnterior pituitary cellsProlactin secretionCalcium/calmodulin-dependent protein kinase IIPituitary cellsCalmodulin-dependent protein kinase IIKN-04KN-62Rat anterior pituitary cellsVoltage-dependent calcium channelsProtein kinase IICalmodulin-dependent protein kinase II inhibitorKCl-stimulated increasesIntracellular calcium increaseCalcium/calmodulin-dependent protein kinase II inhibitorKinase II inhibitorCalcium channelsCalcium increaseIntracellular Ca2Kinase IISecretionInhibits activationII inhibitorsIC50InhibitorsCells
1989
Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin
Law G, Pachter J, Dannies P. Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin. Endocrinology 1989, 3: 539-546. PMID: 2501668, DOI: 10.1210/mend-3-3-539.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCells, CulturedFemalePituitary Gland, AnteriorProlactinRatsRats, Inbred StrainsThyrotropin-Releasing HormoneConceptsPulses of TRHPRL releasePRL secretionCytosolic free calcium concentrationPost-receptor stepsRat anterior pituitary glandThyrotropin-Releasing HormoneRelease of prolactinIndicator indo-1Anterior pituitary glandFree calcium concentrationRelationship of changesContinuous administrationSustained elevationNM TRHPituitary glandIndo-1TRHFluorescent Ca2Calcium concentrationRefractory stateTransient spikeSecretionMinRelease
1988
Expression of messenger ribonucleic acids encoding a parathyroid hormone-like peptide in normal human and animal tissues with abnormal expression in human parathyroid adenomas.
Ikeda K, Weir E, Mangin M, Dannies P, Kinder B, Deftos L, Brown E, Broadus A. Expression of messenger ribonucleic acids encoding a parathyroid hormone-like peptide in normal human and animal tissues with abnormal expression in human parathyroid adenomas. Endocrinology 1988, 2: 1230-6. PMID: 3216862, DOI: 10.1210/mend-2-12-1230.Peer-Reviewed Original ResearchConceptsPTH-like peptideHuman parathyroid adenomasParathyroid adenomaAbnormal human parathyroid tissueHuman parathyroid tissueNumber of endocrineMedullary carcinoma cellsParathyroid hormone-like peptideExpression of mRNAAutonomous glandsHumoral hypercalcemiaParathyroid tissueHormone-like peptideAdrenal cortexRat brainNonendocrine tissuesAdrenal medullaBone marrowMessenger ribonucleic acidStomach mucosaHuman osteosarcomaOverexpression of transcriptsRat pituitaryNormal humansAbnormal expression
1986
Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures
Martinez-Campos A, Amara J, Dannies P. Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures. Molecular And Cellular Endocrinology 1986, 48: 127-133. PMID: 3803705, DOI: 10.1016/0303-7207(86)90035-3.Peer-Reviewed Original ResearchConceptsProlactin productionPituitary cellsMale rat anterior pituitary cellsRat anterior pituitary cellsPartial estrogen agonistNormal pituitary cellsAnterior pituitary cellsConcentrations of estradiolDose-response relationshipPrimary pituitary culturesEstrogen antagonistLY 117018Estrogen agonistM tamoxifenPartial agonistPituitary culturesEstrogen inductionTamoxifenMaximal stimulationFull antagonistM LysPrimary culturesStimulationPimozideAgonistsRat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site.
Strobl J, Dannies P, Thompson E. Rat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site. Biochemistry 1986, 25: 3640-8. PMID: 3013292, DOI: 10.1021/bi00360a025.Peer-Reviewed Original ResearchConceptsTranscription initiation siteInitiation siteGene expressionRat growth hormone gene expressionMethylation-sensitive restriction enzymesRat growth hormone geneHybrid cellsGene control regionsGrowth hormone geneThai sitesGrowth hormone gene expressionGH3 cellsCGCG sequenceControl regionDNA methylationBp 5Gene regionGH geneHormone geneHormone gene expressionRestriction enzymesMethylation statusRestriction sitesMouse fibroblastsGH gene expressionA possible differentiation of anterior pituitary cells in collagen gels into neurons
Martinez-Campos A, Dannies P. A possible differentiation of anterior pituitary cells in collagen gels into neurons. Cell And Tissue Research 1986, 244: 21-26. PMID: 2421915, DOI: 10.1007/bf00218377.Peer-Reviewed Original Research
1985
Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures
Delbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.Peer-Reviewed Original ResearchStimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*
DELBEKE D, DANNIES P. Stimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*. Endocrinology 1985, 117: 439-446. PMID: 2990850, DOI: 10.1210/endo-117-2-439.Peer-Reviewed Original ResearchConceptsPRL releaseDopaminergic inhibitionAbsence of dopamineMessenger systemsRat anterior pituitary cellsPattern of releaseAnterior pituitary cellsCAMP messenger systemMin of preincubationBasal releaseProlactin releaseNM TRHPituitary cellsIntracellular Ca2Sustained phasePhorbol ester 12Cytosolic Ca2Intracellular cAMPTRHAdenylate cyclaseCAMP levelsDopamineSustained releaseLevel of releaseC-kinase activity
1984
Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*
DELBEKE D, SCAMMELI J, DANNIES P. Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*. Endocrinology 1984, 114: 1433-1440. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPRL releaseMale rat pituitary glandPrimary culturesNormal cellsNormal pituitary cellsPresence of D600Release of prolactinRat pituitary glandAddition of forskolinIntracellular cAMP concentrationPituitary tumorsFischer ratsPituitary cellsPituitary glandChannel inhibitorsTumor cellsTypes of cellsAdenylate cyclaseD600ForskolinCAMP concentrationCalcium requirementForskolin stimulationCellsDepletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*
SCAMMELL J, DANNIES P. Depletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*. Endocrinology 1984, 114: 712-716. PMID: 6697959, DOI: 10.1210/endo-114-3-712.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCysteamineFemaleImmune SeraImmunoassayPituitary NeoplasmsProlactinRatsRats, Inbred F344Spiperone: evidence for uptake into secretory granules.
Dannies P, Rudnick M, Fishkes H, Rudnick G. Spiperone: evidence for uptake into secretory granules. Proceedings Of The National Academy Of Sciences Of The United States Of America 1984, 81: 1867-1870. PMID: 6584920, PMCID: PMC345023, DOI: 10.1073/pnas.81.6.1867.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportButyrophenonesCells, CulturedCytoplasmic GranulesKineticsPituitary GlandRatsSpiperoneConceptsSerotonin receptorsSecretory granulesRat pituitary tumor cellsMicroM reserpinePlatelet dense granulesPituitary tumor cellsDopamine antagonistsIntracellular storage organellesSpiperoneTumor cellsSerotonin transportDense granulesBiogenic amine transportReceptorsCell strainsDopamineEquimolar concentrationsSpecific ligandsAmine transportStorage organellesPlasma membrane transport systemsReserpineAntagonistMinNeurons
1983
Veratridine and Ouabain Stimulate Calcium-Dependent Prolactin Release*
SCAMMELL J, DANNIES P. Veratridine and Ouabain Stimulate Calcium-Dependent Prolactin Release*. Endocrinology 1983, 113: 1228-1235. PMID: 6617570, DOI: 10.1210/endo-113-4-1228.Peer-Reviewed Original ResearchConceptsPRL releaseCalcium channel blocker D600Rat anterior pituitary cellsTetrodotoxin-insensitive mechanismCalcium channel blockersChannel blocker tetrodotoxinDopaminergic agonist bromocriptineEffect of veratridineAnterior pituitary cellsAction of veratridineChannel blocker D600Blocker tetrodotoxinAgonist bromocriptineProlactin releaseChannel blockersVeratridine effectPituitary cellsChannel activatorVeratridineGH4C1 cellsPrimary culturesOuabainNormal cellsStimulationPreincubationSynthesis and biological activity of 5-fluoroimidazole-TRH
Labroo V, Kirk K, Cohen L, Delbeke D, Dannies P. Synthesis and biological activity of 5-fluoroimidazole-TRH. Biochemical And Biophysical Research Communications 1983, 113: 581-585. PMID: 6409106, DOI: 10.1016/0006-291x(83)91765-5.Peer-Reviewed Original Research
1981
Muscarinic inhibition of prolactin production in cultures of rat pituitary cells
Rudnick M, Dannies P. Muscarinic inhibition of prolactin production in cultures of rat pituitary cells. Biochemical And Biophysical Research Communications 1981, 101: 689-696. PMID: 7306103, DOI: 10.1016/0006-291x(81)91313-9.Peer-Reviewed Original Research
1980
Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells.
Tam S, Dannies P. Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells. Journal Of Biological Chemistry 1980, 255: 6595-6599. PMID: 6771274, DOI: 10.1016/s0021-9258(18)43611-3.Peer-Reviewed Original ResearchEffects of Estradiol on Prolactin Production and Dihydroergocryptine-Induced Inhibition of Prolactin Production in Primary Cultures of Rat Pituitary Cells*
WEST B, DANNIES P. Effects of Estradiol on Prolactin Production and Dihydroergocryptine-Induced Inhibition of Prolactin Production in Primary Cultures of Rat Pituitary Cells*. Endocrinology 1980, 106: 1108-1113. PMID: 6766855, DOI: 10.1210/endo-106-4-1108.Peer-Reviewed Original Research2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment.
Dannies P, Rudnick M. 2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment. Journal Of Biological Chemistry 1980, 255: 2776-2781. PMID: 7358708, DOI: 10.1016/s0021-9258(19)85806-4.Peer-Reviewed Original ResearchDifferential Ability of Thyrotropin-Releasing Hormone and N3im-Methyl-Thyrotropin-Releasing Hormone to Affect Prolactin and Thyrotropin Production in Primary Rat Pituitary Cell Cultures*
DANNIES P, MARKELL M. Differential Ability of Thyrotropin-Releasing Hormone and N3im-Methyl-Thyrotropin-Releasing Hormone to Affect Prolactin and Thyrotropin Production in Primary Rat Pituitary Cell Cultures*. Endocrinology 1980, 106: 107-112. PMID: 6765928, DOI: 10.1210/endo-106-1-107.Peer-Reviewed Original Research
1979
Antipsychotic Drugs Inhibit Prolactin Release from Rat Anterior Pituitary Cells in Culture by a Mechanism Not Involving the Dopamine Receptor*
WEST B, DANNIES P. Antipsychotic Drugs Inhibit Prolactin Release from Rat Anterior Pituitary Cells in Culture by a Mechanism Not Involving the Dopamine Receptor*. Endocrinology 1979, 104: 877-880. PMID: 436760, DOI: 10.1210/endo-104-4-877.Peer-Reviewed Original ResearchConceptsRat anterior pituitary cellsAnterior pituitary cellsGH releasePRL releaseControl valuesAntipsychotic drugsDopamine antagonistsDopamine receptorsPituitary cellsInhibition of PRLMicroM pimozideDopamine agonistsHormone releaseD-butaclamolInhibited secretionPRLBromocriptineAntagonistButaclamolInhibitionReceptorsDrugsReleaseCellsHaloperidol