2004
Aggregation and Lack of Secretion of Most Newly Synthesized Proinsulin in Non-β-Cell Lines
Zhu YL, Abdo A, Gesmonde JF, Zawalich KC, Zawalich W, Dannies PS. Aggregation and Lack of Secretion of Most Newly Synthesized Proinsulin in Non-β-Cell Lines. Endocrinology 2004, 145: 3840-3849. PMID: 15117881, DOI: 10.1210/en.2003-1512.Peer-Reviewed Original Research
2002
Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type II
Zhu YL, Conway-Campbell B, Waters MJ, Dannies PS. Prolonged Retention after Aggregation into Secretory Granules of Human R183H-Growth Hormone (GH), a Mutant that Causes Autosomal Dominant GH Deficiency Type II. Endocrinology 2002, 143: 4243-4248. PMID: 12399418, DOI: 10.1210/en.2002-220575.Peer-Reviewed Original Research
2000
Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH
Lee M, Wajnrajch M, Kim S, Plotnick L, Wang J, Gertner J, Leibel R, Dannies P. Autosomal Dominant Growth Hormone (GH) Deficiency Type II: The Del32–71-GH Deletion Mutant Suppresses Secretion of Wild-Type GH. Endocrinology 2000, 141: 883-890. PMID: 10698162, DOI: 10.1210/endo.141.3.7380.Peer-Reviewed Original ResearchConceptsWild-type GHSecretory pathway functionNeuroendocrine cell lineGH deficiency type IISuppression of accumulationPathway functionTransient transfectionIntracellular stabilityCHO cellsAutosomal dominant formCell linesDecreased stabilityNormal allelePosttranslational effectGeneral suppressionCoexpressionProtein
1997
Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+.
Sun Z, Lee M, Rhee H, Arrandale J, Dannies P. Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+. Endocrinology 1997, 11: 1544-51. PMID: 9280069, DOI: 10.1210/mend.11.10.0002.Peer-Reviewed Original Research
1995
Biological activity and immunological reactivity of human prolactin mutants
Rhee H, Sun Z, Kim S, Goffin V, Martial J, Dannies P. Biological activity and immunological reactivity of human prolactin mutants. Endocrinology 1995, 136: 4990-4995. PMID: 7588233, DOI: 10.1210/endo.136.11.7588233.Peer-Reviewed Original ResearchConceptsSerine 90Cystine loopBiological activityRat pituitary cell lineHuman PRLPituitary cell lineWild typeCertain fishPRL-3MutantsTryptophan fluorescenceCell linesMutationsFishT mutationCell assayPRL storageImmunological reactivityImmunological propertiesSerineConcentration of ureaActivityConservationSpacial requirementsLoop
1994
Inhibition of rat prolactin (PRL) storage by coexpression of human PRL.
Arrandale J, Dannies P. Inhibition of rat prolactin (PRL) storage by coexpression of human PRL. Endocrinology 1994, 8: 1083-1090. PMID: 7997234, DOI: 10.1210/mend.8.8.7997234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAsparagineBiological TransportCell CompartmentationCytoplasmic GranulesEpidermal Growth FactorEstradiolGene ExpressionHumansInsulinModels, MolecularPituitary NeoplasmsProlactinProtein ConformationRatsRecombinant Fusion ProteinsSerineSpecies SpecificityTransfectionTumor Cells, CulturedConceptsHuman PRLRat PRLGH4C1 cellsReceptor-mediated mechanismNM epidermal growth factorSecretory granulesNM estradiolDense-core secretory granulesEpidermal growth factorHormone treatmentPRL storageProlactin storagePRLGrowth factorNM insulinMore ratsUntransfected cellsRatsRegulated pathwayControl culturesCellsInhibitionMarked specificity