1996
KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells
Cui Z, Hidaka H, Dannies P. KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells. Biochimica Et Biophysica Acta 1996, 1310: 343-347. PMID: 8599613, DOI: 10.1016/0167-4889(95)00170-0.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsCalciumCalcium-Calmodulin-Dependent Protein KinasesCells, CulturedEnzyme InhibitorsFemaleIsoquinolinesKineticsPiperazinesPituitary Gland, AnteriorPotassiumPotassium ChlorideProlactinRatsRats, Sprague-DawleyThyrotropin-Releasing HormoneTime FactorsConceptsAnterior pituitary cellsProlactin secretionCalcium/calmodulin-dependent protein kinase IIPituitary cellsCalmodulin-dependent protein kinase IIKN-04KN-62Rat anterior pituitary cellsVoltage-dependent calcium channelsProtein kinase IICalmodulin-dependent protein kinase II inhibitorKCl-stimulated increasesIntracellular calcium increaseCalcium/calmodulin-dependent protein kinase II inhibitorKinase II inhibitorCalcium channelsCalcium increaseIntracellular Ca2Kinase IISecretionInhibits activationII inhibitorsIC50InhibitorsCells
1992
Thyrotropin-releasing hormone-mediated Mn2+ entry in perifused rat anterior pituitary cells
Cui Z, Dannies P. Thyrotropin-releasing hormone-mediated Mn2+ entry in perifused rat anterior pituitary cells. Biochemical Journal 1992, 283: 507-513. PMID: 1575695, PMCID: PMC1131064, DOI: 10.1042/bj2830507.Peer-Reviewed Original ResearchConceptsAnterior pituitary cellsRat anterior pituitary cellsPituitary cellsMicroM dopaminePerifused anterior pituitary cellsIndo-1-loaded cellsReceptor-mediated Ca2Action of TRHContinuous TRH infusionTRH infusionCation entryIntracellular Ca2Indo-1TRHTumor promotorFree mediumG proteinsUnstimulated cellsCell typesDopamineCa2CellsLittle effectInsensitive wavelengthInfusion
1991
Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion
Pachter J, Law G, Dannies P. Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion. European Journal Of Pharmacology 1991, 195: 373-379. PMID: 1714395, DOI: 10.1016/0014-2999(91)90478-9.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCalciumCalcium Channel AgonistsCalcium Channel BlockersCalcium RadioisotopesDihydropyridinesFemaleIn Vitro TechniquesInositol PhosphatesNimodipinePerfusionProlactinPyridinesRatsRats, Inbred StrainsReceptors, PurinergicThyrotropin-Releasing HormoneConceptsBay K 8644Basal prolactin secretionProlactin secretionChannel activator Bay K 8644Potentiation of TRHChannel antagonist nimodipineAnterior pituitary cellsAnterior pituitary tumor cellsEnhancement of basalAccumulation of inositolSDZ 202Antagonist nimodipinePituitary tumor cellsInositol polyphosphate accumulationCGP 28392Dihydropyridine Ca2Hormone secretionChannel agonistDihydropyridine agonistPituitary cellsTRHTumor cellsSecretionGH4C1 cellsPrimary cultures
1990
Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells
Law G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCaffeineCalciumCell LineInositol PhosphatesKineticsNimodipinePituitary NeoplasmsPlants, MedicinalProlactinTerpenesThapsigarginThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsThyrotropin-releasing hormoneGH4C1 pituitary cellsPituitary cellsIntracellular storesGH4C1 cellsAbility of TRHBay K 8644Cytosolic free Ca2Action of thapsigarginInsP3-sensitive storesSustained elevationChannel blockersReceptor levelsTRH stimulationExtracellular Ca2Intracellular Ca2Transient increaseFree Ca2ThapsigarginSubsequent spikesHormoneCell typesCa2CellsEffluxComparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1989
Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin
Law G, Pachter J, Dannies P. Ca2+ Transients Induced by Thyrotropin-Releasing Hormone Rapidly Lose Their Ability to Cause Release of Prolactin. Endocrinology 1989, 3: 539-546. PMID: 2501668, DOI: 10.1210/mend-3-3-539.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCells, CulturedFemalePituitary Gland, AnteriorProlactinRatsRats, Inbred StrainsThyrotropin-Releasing HormoneConceptsPulses of TRHPRL releasePRL secretionCytosolic free calcium concentrationPost-receptor stepsRat anterior pituitary glandThyrotropin-Releasing HormoneRelease of prolactinIndicator indo-1Anterior pituitary glandFree calcium concentrationRelationship of changesContinuous administrationSustained elevationNM TRHPituitary glandIndo-1TRHFluorescent Ca2Calcium concentrationRefractory stateTransient spikeSecretionMinRelease
1988
TRH and BAY K 8644 synergistically stimulate prolactin release but not 45Ca2+ uptake
Pachter J, Law G, Dannies P. TRH and BAY K 8644 synergistically stimulate prolactin release but not 45Ca2+ uptake. American Journal Of Physiology 1988, 255: c633-c640. PMID: 2461093, DOI: 10.1152/ajpcell.1988.255.5.c633.Peer-Reviewed Original ResearchConceptsBay K 8644Thyrotropin-releasing hormoneProlactin secretionAngiotensin IIProlactin releaseChannel agonist Bay K 8644Agonist Bay K 8644Rat anterior pituitary cellsFluorescent indicator indo-1Anterior pituitary cellsDihydropyridine-sensitive Ca2Indicator indo-1Primary cultured cellsSecretory peaksPituitary cellsSubsequent stimulationIndo-1Transient increaseCytosolic Ca2GH4C1 cellsPrimary culturesSecretionUntreated cellsAdditional effectCultured cellsDopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells
Law G, Pachter J, Dannies P. Dopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells. Endocrinology 1988, 2: 966-972. PMID: 3141796, DOI: 10.1210/mend-2-10-966.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCentrifugationDopamineFemaleInositol PhosphatesPerfusionPituitary Gland, AnteriorRatsRats, Inbred F344Rats, Inbred StrainsThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsRat anterior pituitary cellsAnterior pituitary cellsPRL secretionPituitary cellsIntracellular Ca2Intracellular storesLow Ca2MM Ca2Inhibition of releaseDopaminergic reductionSource of Ca2PRL releaseIntracellular calciumNM TRHPerifusion systemExtracellular Ca2MicroM dopamineTRHInternal Ca2DopamineEnriched populationMM KClSecretionInositol phosphatesCa2Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH
Pachter J, Law G, Dannies P. Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH. Biochemical And Biophysical Research Communications 1988, 154: 654-659. PMID: 3135808, DOI: 10.1016/0006-291x(88)90189-1.Peer-Reviewed Original ResearchAnimalsBombesinCell LineInositol PhosphatesKineticsPituitary NeoplasmsRatsSugar PhosphatesThyrotropin-Releasing Hormone
1986
Dopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*
DELBEKE P, SCAMMELL J, MARTINEZ-CAMPOS A, DANNIES P. Dopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*. Endocrinology 1986, 118: 1271-1277. PMID: 3004906, DOI: 10.1210/endo-118-4-1271.Peer-Reviewed Original ResearchConceptsPRL releaseIntracellular cAMP concentrationFemale Fischer 344 ratsCAMP levelsAnterior pituitary cellsCAMP concentrationFischer 344 ratsPRL accumulationPercentage of lactotrophsDopamine inhibitsPituitary tumorsContinuous Percoll density gradientsNM TRHPituitary cellsCAMP accumulationMicroM dopaminePercoll density gradientsBasal levelsMonophosphate levelsMin of incubationLactotrophsAdenylate cyclasePrimary culturesDopamineForskolin
1985
Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures
Delbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcimycinCell LineCells, CulturedFemaleKineticsPituitary Gland, AnteriorPituitary NeoplasmsProlactinRatsRats, Inbred StrainsTetradecanoylphorbol AcetateThyrotropin-Releasing Hormone
1984
Unresponsiveness of GH cells to cyclo(histidyl-proline), a metabolite of thyrotropin releasing hormone
Battaini F, Dannies P, Peterkofsky A. Unresponsiveness of GH cells to cyclo(histidyl-proline), a metabolite of thyrotropin releasing hormone. Life Sciences 1984, 35: 2519-2527. PMID: 6096658, DOI: 10.1016/0024-3205(84)90438-7.Peer-Reviewed Original ResearchSynergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin
Delbeke D, Kojima I, Dannies P, Rasmussen H. Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin. Biochemical And Biophysical Research Communications 1984, 123: 735-741. PMID: 6091639, DOI: 10.1016/0006-291x(84)90291-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcimycinCalciumCell LineColforsinCyclic AMPDiterpenesDrug SynergismPhorbolsPituitary NeoplasmsProlactinTetradecanoylphorbol AcetateThyrotropin-Releasing HormoneTime Factors
1983
Synthesis and biological activity of 5-fluoroimidazole-TRH
Labroo V, Kirk K, Cohen L, Delbeke D, Dannies P. Synthesis and biological activity of 5-fluoroimidazole-TRH. Biochemical And Biophysical Research Communications 1983, 113: 581-585. PMID: 6409106, DOI: 10.1016/0006-291x(83)91765-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCells, CulturedChemical PhenomenaChemistryPituitary GlandProlactinRatsThyrotropin-Releasing Hormone
1982
Prolactin: Multiple intracellular processing routes plus several potential mechanisms for regulation
Dannies P. Prolactin: Multiple intracellular processing routes plus several potential mechanisms for regulation. Biochemical Pharmacology 1982, 31: 2845-2849. PMID: 6291547, DOI: 10.1016/0006-2952(82)90253-2.Peer-Reviewed Original ResearchAnimalsBromocriptineCalciumCyclic AMPEndoplasmic ReticulumProlactinRatsThyrotropin-Releasing HormoneHistidyl-proline diketopiperazine: its biological role as a regulatory peptide
Peterkofsky A, Battaini F, Koch Y, Takahara Y, Dannies P. Histidyl-proline diketopiperazine: its biological role as a regulatory peptide. Molecular And Cellular Biochemistry 1982, 42: 45-63. PMID: 6278283, DOI: 10.1007/bf00223538.Peer-Reviewed Original ResearchConceptsHistidyl-proline diketopiperazineCentral nervous systemAdrenal cortex membranesCyclic nucleotide levelsCNS depressionSpecific membrane receptorsCortex membranesProlactin releaseRat brainClassical neurotransmittersNervous systemPituitary cellsTRHSuch receptorsRegulatory peptidesPituitaryStereotypic behaviorImmunological methodsSpecific bindingBrainMembrane receptorsReceptorsDistribution studiesPossible mechanismNucleotide level
1980
Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*
DANNIES P, TASHJIAN A. Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*. Endocrinology 1980, 106: 1532-1536. PMID: 6244936, DOI: 10.1210/endo-106-5-1532.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCholera ToxinCyclic AMPGrowth HormoneKineticsPituitary GlandProlactinRatsThyrotropin-Releasing HormoneConceptsGH synthesisCholera toxinIntracellular cAMPWeeks of treatmentThyrotropin-releasing hormoneRelease of PRLHormone synthesisRat pituitary cellsIntracellular PRLGrowth hormone synthesisPRL releaseSimilar lag periodPituitary cellsGH cellsControl valuesPRL synthesisTRHPRLToxinClonal strainsLag periodReleaseCellsCAMPDecreaseHormonal control of tyrosine aminotransferase, prolactin, and growth hormone induction in somatic cell hybrids
Thompson E, Dannies P, Buckler C, Tashjian A. Hormonal control of tyrosine aminotransferase, prolactin, and growth hormone induction in somatic cell hybrids. The Journal Of Steroid Biochemistry And Molecular Biology 1980, 12: 193-210. PMID: 6106733, DOI: 10.1016/0022-4731(80)90269-1.Peer-Reviewed Original ResearchDifferential Ability of Thyrotropin-Releasing Hormone and N3im-Methyl-Thyrotropin-Releasing Hormone to Affect Prolactin and Thyrotropin Production in Primary Rat Pituitary Cell Cultures*
DANNIES P, MARKELL M. Differential Ability of Thyrotropin-Releasing Hormone and N3im-Methyl-Thyrotropin-Releasing Hormone to Affect Prolactin and Thyrotropin Production in Primary Rat Pituitary Cell Cultures*. Endocrinology 1980, 106: 107-112. PMID: 6765928, DOI: 10.1210/endo-106-1-107.Peer-Reviewed Original ResearchAnimalsCells, CulturedFemaleKineticsPituitary Gland, AnteriorProlactinRatsThyrotropinThyrotropin-Releasing Hormone
1976
Release and synthesis of prolactin by rat pituitary cell strains are regulated independently by thyrotropin-releasing hormone
DANNIES P, TASJIAN A. Release and synthesis of prolactin by rat pituitary cell strains are regulated independently by thyrotropin-releasing hormone. Nature 1976, 261: 707-710. PMID: 180421, DOI: 10.1038/261707a0.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedClone CellsDose-Response Relationship, DrugPituitary GlandProlactinReceptors, Cell SurfaceThyrotropin-Releasing HormoneTime Factors