1995
Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage
Mastro R, Dannies P. Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage. Endocrinology 1995, 136: 69-74. PMID: 7828559, DOI: 10.1210/endo.136.1.7828559.Peer-Reviewed Original ResearchConceptsEpidermal growth factorNormal lactotrophsGH4C1 cellsGrowth factorForms of PRLFemale rat pituitary glandsNM epidermal growth factorRat pituitary glandDense core granulesRat pituitary tumor cellsPRL storagePituitary tumor cellsNM estradiolTotal PRLPituitary glandPRLTumor cellsCore granulesInsulinNM insulinLack of correlationLactotrophs
1994
Inhibition of rat prolactin (PRL) storage by coexpression of human PRL.
Arrandale J, Dannies P. Inhibition of rat prolactin (PRL) storage by coexpression of human PRL. Endocrinology 1994, 8: 1083-1090. PMID: 7997234, DOI: 10.1210/mend.8.8.7997234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAsparagineBiological TransportCell CompartmentationCytoplasmic GranulesEpidermal Growth FactorEstradiolGene ExpressionHumansInsulinModels, MolecularPituitary NeoplasmsProlactinProtein ConformationRatsRecombinant Fusion ProteinsSerineSpecies SpecificityTransfectionTumor Cells, CulturedConceptsHuman PRLRat PRLGH4C1 cellsReceptor-mediated mechanismNM epidermal growth factorSecretory granulesNM estradiolDense-core secretory granulesEpidermal growth factorHormone treatmentPRL storageProlactin storagePRLGrowth factorNM insulinMore ratsUntransfected cellsRatsRegulated pathwayControl culturesCellsInhibitionMarked specificity
1990
Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated.
Reaves B, Van Itallie C, Moore H, Dannies P. Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated. Endocrinology 1990, 4: 1017-26. PMID: 2284004, DOI: 10.1210/mend-4-7-1017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNAGene Expression RegulationMicePituitary NeoplasmsProinsulinProlactinRNA, MessengerTumor Cells, CulturedConceptsGH4C1 cellsEpidermal growth factorPRL synthesisGrowth factorPreferential increaseCombination of estradiolRegulated pathwayRat pituitary tumor cellsIntracellular PRLPituitary tumor cellsBasal releasePRL releaseIntracellular proinsulinSame time courseHormone treatmentPRL storageTumor cellsProinsulin immunoreactivityMRNA levelsPRLInsulinSame peak heightHormoneProinsulinTreatmentThapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells
Law G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCaffeineCalciumCell LineInositol PhosphatesKineticsNimodipinePituitary NeoplasmsPlants, MedicinalProlactinTerpenesThapsigarginThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsThyrotropin-releasing hormoneGH4C1 pituitary cellsPituitary cellsIntracellular storesGH4C1 cellsAbility of TRHBay K 8644Cytosolic free Ca2Action of thapsigarginInsP3-sensitive storesSustained elevationChannel blockersReceptor levelsTRH stimulationExtracellular Ca2Intracellular Ca2Transient increaseFree Ca2ThapsigarginSubsequent spikesHormoneCell typesCa2CellsEffluxComparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1988
Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH
Pachter J, Law G, Dannies P. Bombesin stimulates inositol polyphosphate production in GH4C1 pituitary tumor cells: Comparison with TRH. Biochemical And Biophysical Research Communications 1988, 154: 654-659. PMID: 3135808, DOI: 10.1016/0006-291x(88)90189-1.Peer-Reviewed Original ResearchEstrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells.
Van Itallie C, Dannies P. Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells. Endocrinology 1988, 2: 332-7. PMID: 2837664, DOI: 10.1210/mend-2-4-332.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DivisionDNADNA, RecombinantElectron Transport Complex IVEnzyme InductionEpidermal Growth FactorEstradiolFemaleInsulinMaleMitochondriaMolecular Sequence DataNucleic Acid HybridizationPituitary NeoplasmsProlactinRatsRats, Inbred StrainsRNA, MessengerTumor Cells, CulturedConceptsCytochrome oxidase II geneCytochrome oxidaseMitochondrial ribonucleic acidMitochondrial genomePituitary tumor cell lineEpidermal growth factorMRNA accumulationNumber of copiesTumor cell linesPituitary tumor cellsCell proliferationMRNACell linesRibonucleic acidMaximal inductionGenesGrowth factorGrowth ratePRL mRNA
1986
Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*
SCAMMELL J, BURRAGE T, DANNIES P. Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*. Endocrinology 1986, 119: 1543-1548. PMID: 3530721, DOI: 10.1210/endo-119-4-1543.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineCytoplasmic GranulesEpidermal Growth FactorEstradiolInsulinMicroscopy, ElectronPituitary NeoplasmsProlactinRatsConceptsGH4C1 cellsIntracellular PRLSecretory granulesCombination of estradiolNumber of granulesPituitary tumor cell linePRL accumulationHormone regimenTumor cell strainsEpidermal growth factorHormone treatmentTumor cell linesPRLGrowth factorControl levelsOccasional granulesCell linesHormonal inductionGHCell strainsCellsGranule numberTreatmentCellular contentStorage granulesRat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site.
Strobl J, Dannies P, Thompson E. Rat growth hormone gene expression is correlated with an unmethylated CGCG sequence near the transcription initiation site. Biochemistry 1986, 25: 3640-8. PMID: 3013292, DOI: 10.1021/bi00360a025.Peer-Reviewed Original ResearchConceptsTranscription initiation siteInitiation siteGene expressionRat growth hormone gene expressionMethylation-sensitive restriction enzymesRat growth hormone geneHybrid cellsGene control regionsGrowth hormone geneThai sitesGrowth hormone gene expressionGH3 cellsCGCG sequenceControl regionDNA methylationBp 5Gene regionGH geneHormone geneHormone gene expressionRestriction enzymesMethylation statusRestriction sitesMouse fibroblastsGH gene expressionDopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*
DELBEKE P, SCAMMELL J, MARTINEZ-CAMPOS A, DANNIES P. Dopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*. Endocrinology 1986, 118: 1271-1277. PMID: 3004906, DOI: 10.1210/endo-118-4-1271.Peer-Reviewed Original ResearchConceptsPRL releaseIntracellular cAMP concentrationFemale Fischer 344 ratsCAMP levelsAnterior pituitary cellsCAMP concentrationFischer 344 ratsPRL accumulationPercentage of lactotrophsDopamine inhibitsPituitary tumorsContinuous Percoll density gradientsNM TRHPituitary cellsCAMP accumulationMicroM dopaminePercoll density gradientsBasal levelsMonophosphate levelsMin of incubationLactotrophsAdenylate cyclasePrimary culturesDopamineForskolin
1985
Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures
Delbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.Peer-Reviewed Original ResearchCysteamine Causes Reduction of Prolactin Monomers Followed by Aggregation in the Rat Pituitary Gland*
SCAMMELL J, BURRAGE T, EISENFELD A, DANNIES P. Cysteamine Causes Reduction of Prolactin Monomers Followed by Aggregation in the Rat Pituitary Gland*. Endocrinology 1985, 116: 2347-2354. PMID: 3996317, DOI: 10.1210/endo-116-6-2347.Peer-Reviewed Original Research
1984
Unresponsiveness of GH cells to cyclo(histidyl-proline), a metabolite of thyrotropin releasing hormone
Battaini F, Dannies P, Peterkofsky A. Unresponsiveness of GH cells to cyclo(histidyl-proline), a metabolite of thyrotropin releasing hormone. Life Sciences 1984, 35: 2519-2527. PMID: 6096658, DOI: 10.1016/0024-3205(84)90438-7.Peer-Reviewed Original ResearchSynergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin
Delbeke D, Kojima I, Dannies P, Rasmussen H. Synergistic stimulation of prolactin release by phorbol ester, A23187 and forskolin. Biochemical And Biophysical Research Communications 1984, 123: 735-741. PMID: 6091639, DOI: 10.1016/0006-291x(84)90291-2.Peer-Reviewed Original ResearchDifference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*
DELBEKE D, SCAMMELI J, DANNIES P. Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*. Endocrinology 1984, 114: 1433-1440. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPRL releaseMale rat pituitary glandPrimary culturesNormal cellsNormal pituitary cellsPresence of D600Release of prolactinRat pituitary glandAddition of forskolinIntracellular cAMP concentrationPituitary tumorsFischer ratsPituitary cellsPituitary glandChannel inhibitorsTumor cellsTypes of cellsAdenylate cyclaseD600ForskolinCAMP concentrationCalcium requirementForskolin stimulationCellsDepletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*
SCAMMELL J, DANNIES P. Depletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*. Endocrinology 1984, 114: 712-716. PMID: 6697959, DOI: 10.1210/endo-114-3-712.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCysteamineFemaleImmune SeraImmunoassayPituitary NeoplasmsProlactinRatsRats, Inbred F344
1983
17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1
AMARA J, DANNIES P. 17β-ESTRADIOL HAS A BIPHASIC EFFECT ON GH CELL GROWTH1. Endocrinology 1983, 112: 1141-1143. PMID: 6822206, DOI: 10.1210/endo-112-3-1141.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DivisionCell LineDose-Response Relationship, DrugEstradiolKineticsPituitary NeoplasmsRatsConceptsHalf-maximal concentrationGH cellsProlactin productionEffects of estradiolPituitary tumor growthDose-dependent decreaseCell numberHigh oestrogen concentrationsRat pituitary tumor cellsPituitary tumor cellsBeta-EstradiolBiphasic effectEstrogen concentrationsTumor growthEstradiolTumor cellsInhibitory effectMaximal concentrationInhibition of growthBiphasic fashionSerumStimulationCell growthHorse serumCells
1982
Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells.
Kiino D, Dannies P. Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells. The Yale Journal Of Biology And Medicine 1982, 55: 409-20. PMID: 6763814, PMCID: PMC2596570.Peer-Reviewed Original ResearchConceptsProlactin storageRat pituitary tumor cellsGH cellsPituitary tumor cellsPituitary functionNM estradiolTumor cellsProlactin synthesisEstradiol-induced increaseCells/NM insulinDays of treatmentProlactin-secreting cellsCell cycle distributionPercent increaseClonal strainsHormone secretionEpidermal growth factorPotassium depolarizationProlactin productionEstradiolHormone productionProlactinInsulinIntracellular prolactin
1981
Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*
KIINO D, DANNIES P. Insulin and 17β-Estradiol Increase the Intracellular Prolactin Content of GH4C1 Cells*. Endocrinology 1981, 109: 1264-1269. PMID: 7026222, DOI: 10.1210/endo-109-4-1264.Peer-Reviewed Original ResearchConceptsIntracellular PRLEffect of insulinDays of treatmentHalf-maximal doseRat pituitary tumor cellsPituitary tumor cellsIntracellular prolactin contentM estradiolEstradiolCombined treatmentPRLInsulinPRL synthesisTumor cellsGH4C1 cellsM insulinControl levelsProlactin contentIntracellular hormoneM. TreatmentTotal proteinTreatmentDoseIntracellular transit timeSmall increaseMuscarinic inhibition of prolactin production in cultures of rat pituitary cells
Rudnick M, Dannies P. Muscarinic inhibition of prolactin production in cultures of rat pituitary cells. Biochemical And Biophysical Research Communications 1981, 101: 689-696. PMID: 7306103, DOI: 10.1016/0006-291x(81)91313-9.Peer-Reviewed Original Research