2002
Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH
Sankoorikal B, Zhu Y, Hodsdon M, Lolis E, Dannies P. Aggregation of Human Wild-Type and H27A-Prolactin in Cells and in Solution: Roles of Zn2+, Cu2+, and pH. Endocrinology 2002, 143: 1302-1309. DOI: 10.1210/en.143.4.1302.Peer-Reviewed Original ResearchAtT20 cellsSecretory granulesFormation of secretory granulesHuman prolactinZn2+-binding siteTransfected AtT20 cellsCores of secretory granulesHuman wild-typeWild-type prolactinAcidic pHCOS cellsRecombinant human prolactinIntracellular compartmentsWild-typeBinding sitesRat prolactinCu2+Neutral pHProlactinRatio of Zn2Concentrations of hormonesAtT20Cells
1990
Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1989
Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent
Law G, Pachter J, Dannies P. Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent. Biochemical And Biophysical Research Communications 1989, 158: 811-816. PMID: 2537637, DOI: 10.1016/0006-291x(89)92794-0.Peer-Reviewed Original ResearchConceptsProlactin releaseAnterior pituitary cellsRelease of prolactinSustained elevated levelsCytosolic free Ca2Amount of prolactinSerial declinePeak Ca2Prolactin secretionPerifusion systemPituitary cellsIndo-1Fluorescent Ca2Repetitive Ca2Transient increaseFree Ca2Elevated levelsProlactinMM KClCa2CellsCoincident increaseMinutesReleaseHigh frequency
1985
Prolactin
Dannies P. Prolactin. 1985, 159-174. DOI: 10.1007/978-1-4684-7018-5_7.Peer-Reviewed Original ResearchSecretion of proteinsRegulation of osmolarityCell biologistsMolecular biologistsHormone actionProlactin productionBiologistsRegulationAnterior pituitary glandVariety of effectsUseful modelHuman growth hormoneHormone productionHuman studiesPituitary glandGrowth hormoneHuman prolactinProteinProlactinReproductionProductionHormoneImportant toolLarge number
1984
Depletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*
SCAMMELL J, DANNIES P. Depletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*. Endocrinology 1984, 114: 712-716. PMID: 6697959, DOI: 10.1210/endo-114-3-712.Peer-Reviewed Original Research
1982
Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells.
Kiino D, Dannies P. Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells. The Yale Journal Of Biology And Medicine 1982, 55: 409-20. PMID: 6763814, PMCID: PMC2596570.Peer-Reviewed Original ResearchConceptsProlactin storageRat pituitary tumor cellsGH cellsPituitary tumor cellsPituitary functionNM estradiolTumor cellsProlactin synthesisEstradiol-induced increaseCells/NM insulinDays of treatmentProlactin-secreting cellsCell cycle distributionPercent increaseClonal strainsHormone secretionEpidermal growth factorPotassium depolarizationProlactin productionEstradiolHormone productionProlactinInsulinIntracellular prolactin
1980
Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells.
Tam S, Dannies P. Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells. Journal Of Biological Chemistry 1980, 255: 6595-6599. PMID: 6771274, DOI: 10.1016/s0021-9258(18)43611-3.Peer-Reviewed Original Research2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment.
Dannies P, Rudnick M. 2-Bromo-alpha-ergocryptine causes degradation of prolactin in primary cultures of rat pituitary cells after chronic treatment. Journal Of Biological Chemistry 1980, 255: 2776-2781. PMID: 7358708, DOI: 10.1016/s0021-9258(19)85806-4.Peer-Reviewed Original Research
1976
Release and synthesis of prolactin by rat pituitary cell strains are regulated independently by thyrotropin-releasing hormone
DANNIES P, TASJIAN A. Release and synthesis of prolactin by rat pituitary cell strains are regulated independently by thyrotropin-releasing hormone. Nature 1976, 261: 707-710. PMID: 180421, DOI: 10.1038/261707a0.Peer-Reviewed Original ResearchThyrotropin-releasing hormone inreases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free system
Dannies P, Tashijian A. Thyrotropin-releasing hormone inreases prolactin mRNA activity in the cytoplasm of GH-cells as measured by translation in a wheat germ cell-free system. Biochemical And Biophysical Research Communications 1976, 70: 1180-1189. PMID: 821486, DOI: 10.1016/0006-291x(76)91027-5.Peer-Reviewed Original Research
1973
Effects of Thyrotropin-releasing Hormone and Hydrocortisone on Synthesis and Degradation of Prolactin in a Rat Pituitary Cell Strain
Dannies P, Tashjian A. Effects of Thyrotropin-releasing Hormone and Hydrocortisone on Synthesis and Degradation of Prolactin in a Rat Pituitary Cell Strain. Journal Of Biological Chemistry 1973, 248: 6174-6179. PMID: 4199262, DOI: 10.1016/s0021-9258(19)43524-2.Peer-Reviewed Original ResearchConceptsSynthesis of prolactinProlactin accumulationThyrotropin-releasing hormoneHydrocortisone resultsEffect of thyrotropinLong-term effectsPituitary cellsGH cellsProlactinDegradation of prolactinHormoneTerm effectsHydrocortisoneIntracellular poolDecreased accumulationClonal strainsCell strainsRadioactive leucineSpecific immunoprecipitationCellsHoursTreatmentDaysLeucineCulture mediumCHAPTER 1 Growth Hormone and Prolactin from Rat Pituitary Tumor Cells11Preparation of this review and the results of original experiments described were supported in part by a research grant from the USPHS (AM 11011). P. S. Dannies is a Postdoctoral Research Fellow of The Arthritis Foundation.
Dannies P, Tashjian A. CHAPTER 1 Growth Hormone and Prolactin from Rat Pituitary Tumor Cells11Preparation of this review and the results of original experiments described were supported in part by a research grant from the USPHS (AM 11011). P. S. Dannies is a Postdoctoral Research Fellow of The Arthritis Foundation. 1973, 561-569. DOI: 10.1016/b978-0-12-427150-0.50128-5.Peer-Reviewed Original ResearchGrowth hormoneRat pituitary tumor cellsPituitary tumor cellsPituitary cellsTumor cellsNormal pituitary cellsMechanism of actionArthritis FoundationIntact animalsComplement fixationProlactinHormoneCell populationsHomogeneous cell populationClonal strainsUseful modelCellsVariety of factorsCell homogenatesMicrocomplement fixationFixationCulture medium