1997
Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+.
Sun Z, Lee M, Rhee H, Arrandale J, Dannies P. Inefficient secretion of human H27A-prolactin, a mutant that does not bind Zn2+. Endocrinology 1997, 11: 1544-51. PMID: 9280069, DOI: 10.1210/mend.11.10.0002.Peer-Reviewed Original Research
1996
KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells
Cui Z, Hidaka H, Dannies P. KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells. Biochimica Et Biophysica Acta 1996, 1310: 343-347. PMID: 8599613, DOI: 10.1016/0167-4889(95)00170-0.Peer-Reviewed Original ResearchMeSH Keywords1-(5-Isoquinolinesulfonyl)-2-MethylpiperazineAnimalsCalciumCalcium-Calmodulin-Dependent Protein KinasesCells, CulturedEnzyme InhibitorsFemaleIsoquinolinesKineticsPiperazinesPituitary Gland, AnteriorPotassiumPotassium ChlorideProlactinRatsRats, Sprague-DawleyThyrotropin-Releasing HormoneTime FactorsConceptsAnterior pituitary cellsProlactin secretionCalcium/calmodulin-dependent protein kinase IIPituitary cellsCalmodulin-dependent protein kinase IIKN-04KN-62Rat anterior pituitary cellsVoltage-dependent calcium channelsProtein kinase IICalmodulin-dependent protein kinase II inhibitorKCl-stimulated increasesIntracellular calcium increaseCalcium/calmodulin-dependent protein kinase II inhibitorKinase II inhibitorCalcium channelsCalcium increaseIntracellular Ca2Kinase IISecretionInhibits activationII inhibitorsIC50InhibitorsCells
1994
Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene
Holt E, Lu C, Dreyer B, Dannies P, Broadus A. Regulation of Parathyroid Hormone‐Related Peptide Gene Expression by Estrogen in GH4C1 Rat Pituitary Cells Has the Pattern of a Primary Response Gene. Journal Of Neurochemistry 1994, 62: 1239-1246. PMID: 8133258, DOI: 10.1046/j.1471-4159.1994.62041239.x.Peer-Reviewed Original ResearchConceptsGH4C1 rat pituitary cellsRat pituitary cellsPituitary cellsParathyroid hormone-related peptide gene expressionPTHrP mRNA expressionPrimary response geneParathyroid hormone-related peptide genePTHrP gene transcriptionPeptide gene expressionResponse genesPharmacological controlMRNA responseMRNA expressionTransient increaseGrowth factorGene transcriptionPlateau responsePeak response
1992
Thyrotropin-releasing hormone-mediated Mn2+ entry in perifused rat anterior pituitary cells
Cui Z, Dannies P. Thyrotropin-releasing hormone-mediated Mn2+ entry in perifused rat anterior pituitary cells. Biochemical Journal 1992, 283: 507-513. PMID: 1575695, PMCID: PMC1131064, DOI: 10.1042/bj2830507.Peer-Reviewed Original ResearchConceptsAnterior pituitary cellsRat anterior pituitary cellsPituitary cellsMicroM dopaminePerifused anterior pituitary cellsIndo-1-loaded cellsReceptor-mediated Ca2Action of TRHContinuous TRH infusionTRH infusionCation entryIntracellular Ca2Indo-1TRHTumor promotorFree mediumG proteinsUnstimulated cellsCell typesDopamineCa2CellsLittle effectInsensitive wavelengthInfusion
1991
Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion
Pachter J, Law G, Dannies P. Ca2+ channel agonists enhance thyrotropin-releasing hormone-induced inositol phosphates and prolactin secretion. European Journal Of Pharmacology 1991, 195: 373-379. PMID: 1714395, DOI: 10.1016/0014-2999(91)90478-9.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCalciumCalcium Channel AgonistsCalcium Channel BlockersCalcium RadioisotopesDihydropyridinesFemaleIn Vitro TechniquesInositol PhosphatesNimodipinePerfusionProlactinPyridinesRatsRats, Inbred StrainsReceptors, PurinergicThyrotropin-Releasing HormoneConceptsBay K 8644Basal prolactin secretionProlactin secretionChannel activator Bay K 8644Potentiation of TRHChannel antagonist nimodipineAnterior pituitary cellsAnterior pituitary tumor cellsEnhancement of basalAccumulation of inositolSDZ 202Antagonist nimodipinePituitary tumor cellsInositol polyphosphate accumulationCGP 28392Dihydropyridine Ca2Hormone secretionChannel agonistDihydropyridine agonistPituitary cellsTRHTumor cellsSecretionGH4C1 cellsPrimary cultures
1990
Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells
Law G, Pachter J, Thastrup O, Hanley M, Dannies P. Thapsigargin, but not caffeine, blocks the ability of thyrotropin-releasing hormone to release Ca2+ from an intracellular store in GH4C1 pituitary cells. Biochemical Journal 1990, 267: 359-364. PMID: 1692207, PMCID: PMC1131296, DOI: 10.1042/bj2670359.Peer-Reviewed Original ResearchMeSH Keywords3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsCaffeineCalciumCell LineInositol PhosphatesKineticsNimodipinePituitary NeoplasmsPlants, MedicinalProlactinTerpenesThapsigarginThyrotropin-Releasing HormoneTumor Cells, CulturedConceptsThyrotropin-releasing hormoneGH4C1 pituitary cellsPituitary cellsIntracellular storesGH4C1 cellsAbility of TRHBay K 8644Cytosolic free Ca2Action of thapsigarginInsP3-sensitive storesSustained elevationChannel blockersReceptor levelsTRH stimulationExtracellular Ca2Intracellular Ca2Transient increaseFree Ca2ThapsigarginSubsequent spikesHormoneCell typesCa2CellsEfflux
1989
Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent
Law G, Pachter J, Dannies P. Ability of repetitive Ca2+ spikes to stimulate prolactin release is frequency dependent. Biochemical And Biophysical Research Communications 1989, 158: 811-816. PMID: 2537637, DOI: 10.1016/0006-291x(89)92794-0.Peer-Reviewed Original ResearchConceptsProlactin releaseAnterior pituitary cellsRelease of prolactinSustained elevated levelsCytosolic free Ca2Amount of prolactinSerial declinePeak Ca2Prolactin secretionPerifusion systemPituitary cellsIndo-1Fluorescent Ca2Repetitive Ca2Transient increaseFree Ca2Elevated levelsProlactinMM KClCa2CellsCoincident increaseMinutesReleaseHigh frequency
1988
TRH and BAY K 8644 synergistically stimulate prolactin release but not 45Ca2+ uptake
Pachter J, Law G, Dannies P. TRH and BAY K 8644 synergistically stimulate prolactin release but not 45Ca2+ uptake. American Journal Of Physiology 1988, 255: c633-c640. PMID: 2461093, DOI: 10.1152/ajpcell.1988.255.5.c633.Peer-Reviewed Original ResearchConceptsBay K 8644Thyrotropin-releasing hormoneProlactin secretionAngiotensin IIProlactin releaseChannel agonist Bay K 8644Agonist Bay K 8644Rat anterior pituitary cellsFluorescent indicator indo-1Anterior pituitary cellsDihydropyridine-sensitive Ca2Indicator indo-1Primary cultured cellsSecretory peaksPituitary cellsSubsequent stimulationIndo-1Transient increaseCytosolic Ca2GH4C1 cellsPrimary culturesSecretionUntreated cellsAdditional effectCultured cellsDopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells
Law G, Pachter J, Dannies P. Dopamine has no Effect on Thyrotropin-Releasing Hormone Mobilization of Calcium from Intracellular Stores in Rat Anterior Pituitary Cells. Endocrinology 1988, 2: 966-972. PMID: 3141796, DOI: 10.1210/mend-2-10-966.Peer-Reviewed Original ResearchConceptsRat anterior pituitary cellsAnterior pituitary cellsPRL secretionPituitary cellsIntracellular Ca2Intracellular storesLow Ca2MM Ca2Inhibition of releaseDopaminergic reductionSource of Ca2PRL releaseIntracellular calciumNM TRHPerifusion systemExtracellular Ca2MicroM dopamineTRHInternal Ca2DopamineEnriched populationMM KClSecretionInositol phosphatesCa2
1986
Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures
Martinez-Campos A, Amara J, Dannies P. Antiestrogens are partial estrogen agonists for prolactin production in primary pituitary cultures. Molecular And Cellular Endocrinology 1986, 48: 127-133. PMID: 3803705, DOI: 10.1016/0303-7207(86)90035-3.Peer-Reviewed Original ResearchConceptsProlactin productionPituitary cellsMale rat anterior pituitary cellsRat anterior pituitary cellsPartial estrogen agonistNormal pituitary cellsAnterior pituitary cellsConcentrations of estradiolDose-response relationshipPrimary pituitary culturesEstrogen antagonistLY 117018Estrogen agonistM tamoxifenPartial agonistPituitary culturesEstrogen inductionTamoxifenMaximal stimulationFull antagonistM LysPrimary culturesStimulationPimozideAgonistsDopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*
DELBEKE P, SCAMMELL J, MARTINEZ-CAMPOS A, DANNIES P. Dopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*. Endocrinology 1986, 118: 1271-1277. PMID: 3004906, DOI: 10.1210/endo-118-4-1271.Peer-Reviewed Original ResearchConceptsPRL releaseIntracellular cAMP concentrationFemale Fischer 344 ratsCAMP levelsAnterior pituitary cellsCAMP concentrationFischer 344 ratsPRL accumulationPercentage of lactotrophsDopamine inhibitsPituitary tumorsContinuous Percoll density gradientsNM TRHPituitary cellsCAMP accumulationMicroM dopaminePercoll density gradientsBasal levelsMonophosphate levelsMin of incubationLactotrophsAdenylate cyclasePrimary culturesDopamineForskolin
1985
Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures
Delbeke D, Kojima I, Dannies P. Comparison of patterns of prolactin release in GH4C1 cells and primary pituitary cultures. Molecular And Cellular Endocrinology 1985, 43: 15-22. PMID: 3934015, DOI: 10.1016/0303-7207(85)90037-1.Peer-Reviewed Original ResearchStimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*
DELBEKE D, DANNIES P. Stimulation of the Adenosine 3′,5′-Monophosphate and the Ca2+ Messenger Systems Together Reverse Dopaminergic Inhibition of Prolactin Release*. Endocrinology 1985, 117: 439-446. PMID: 2990850, DOI: 10.1210/endo-117-2-439.Peer-Reviewed Original ResearchConceptsPRL releaseDopaminergic inhibitionAbsence of dopamineMessenger systemsRat anterior pituitary cellsPattern of releaseAnterior pituitary cellsCAMP messenger systemMin of preincubationBasal releaseProlactin releaseNM TRHPituitary cellsIntracellular Ca2Sustained phasePhorbol ester 12Cytosolic Ca2Intracellular cAMPTRHAdenylate cyclaseCAMP levelsDopamineSustained releaseLevel of releaseC-kinase activity
1984
Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*
DELBEKE D, SCAMMELI J, DANNIES P. Difference in Calcium Requirements for Forskolin-Induced Release of Prolactin from Normal Pituitary Cells and GH4C1 Cells in Culture*. Endocrinology 1984, 114: 1433-1440. PMID: 6323146, DOI: 10.1210/endo-114-4-1433.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPRL releaseMale rat pituitary glandPrimary culturesNormal cellsNormal pituitary cellsPresence of D600Release of prolactinRat pituitary glandAddition of forskolinIntracellular cAMP concentrationPituitary tumorsFischer ratsPituitary cellsPituitary glandChannel inhibitorsTumor cellsTypes of cellsAdenylate cyclaseD600ForskolinCAMP concentrationCalcium requirementForskolin stimulationCellsDepletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*
SCAMMELL J, DANNIES P. Depletion of Pituitary Prolactin by Cysteamine Is due to Loss of Immunological Activity*. Endocrinology 1984, 114: 712-716. PMID: 6697959, DOI: 10.1210/endo-114-3-712.Peer-Reviewed Original Research
1983
Veratridine and Ouabain Stimulate Calcium-Dependent Prolactin Release*
SCAMMELL J, DANNIES P. Veratridine and Ouabain Stimulate Calcium-Dependent Prolactin Release*. Endocrinology 1983, 113: 1228-1235. PMID: 6617570, DOI: 10.1210/endo-113-4-1228.Peer-Reviewed Original ResearchConceptsPRL releaseCalcium channel blocker D600Rat anterior pituitary cellsTetrodotoxin-insensitive mechanismCalcium channel blockersChannel blocker tetrodotoxinDopaminergic agonist bromocriptineEffect of veratridineAnterior pituitary cellsAction of veratridineChannel blocker D600Blocker tetrodotoxinAgonist bromocriptineProlactin releaseChannel blockersVeratridine effectPituitary cellsChannel activatorVeratridineGH4C1 cellsPrimary culturesOuabainNormal cellsStimulationPreincubation
1982
Histidyl-proline diketopiperazine: its biological role as a regulatory peptide
Peterkofsky A, Battaini F, Koch Y, Takahara Y, Dannies P. Histidyl-proline diketopiperazine: its biological role as a regulatory peptide. Molecular And Cellular Biochemistry 1982, 42: 45-63. PMID: 6278283, DOI: 10.1007/bf00223538.Peer-Reviewed Original ResearchConceptsHistidyl-proline diketopiperazineCentral nervous systemAdrenal cortex membranesCyclic nucleotide levelsCNS depressionSpecific membrane receptorsCortex membranesProlactin releaseRat brainClassical neurotransmittersNervous systemPituitary cellsTRHSuch receptorsRegulatory peptidesPituitaryStereotypic behaviorImmunological methodsSpecific bindingBrainMembrane receptorsReceptorsDistribution studiesPossible mechanismNucleotide level
1981
Muscarinic inhibition of prolactin production in cultures of rat pituitary cells
Rudnick M, Dannies P. Muscarinic inhibition of prolactin production in cultures of rat pituitary cells. Biochemical And Biophysical Research Communications 1981, 101: 689-696. PMID: 7306103, DOI: 10.1016/0006-291x(81)91313-9.Peer-Reviewed Original Research
1980
Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells.
Tam S, Dannies P. Dopaminergic inhibition of ionophore A23187-stimulated release of prolactin from rat anterior pituitary cells. Journal Of Biological Chemistry 1980, 255: 6595-6599. PMID: 6771274, DOI: 10.1016/s0021-9258(18)43611-3.Peer-Reviewed Original ResearchAction of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*
DANNIES P, TASHJIAN A. Action of Cholera Toxin on Hormone Synthesis and Release in GH Cells: Evidence that Adenosine 3′,5′-Monophosphate Does Not Mediate the Decrease in Growth Hormone Synthesis Caused by Thyrotropin-Releasing Hormone*. Endocrinology 1980, 106: 1532-1536. PMID: 6244936, DOI: 10.1210/endo-106-5-1532.Peer-Reviewed Original ResearchConceptsGH synthesisCholera toxinIntracellular cAMPWeeks of treatmentThyrotropin-releasing hormoneRelease of PRLHormone synthesisRat pituitary cellsIntracellular PRLGrowth hormone synthesisPRL releaseSimilar lag periodPituitary cellsGH cellsControl valuesPRL synthesisTRHPRLToxinClonal strainsLag periodReleaseCellsCAMPDecrease