2004
Is there structural specificity in the reversible protein aggregates that are stored in secretory granules?
Keeler C, Hodsdon ME, Dannies PS. Is there structural specificity in the reversible protein aggregates that are stored in secretory granules? Journal Of Molecular Neuroscience 2004, 22: 43-49. PMID: 14742909, DOI: 10.1385/jmn:22:1-2:43.Peer-Reviewed Original ResearchConceptsSecretory granule proteinsMembrane proteinsGranule functionGranule proteinsAmino acid residuesSecretory granulesExcess membraneProtein aggregatesSecretory proteinsAcid residuesProteinStructural specificityResiduesSurface motifsGranulesAggregation processMembraneReversible aggregationSpecificityMotifNMR spectroscopyAggregationAggregatesCellsAccumulation
2001
Misfolded growth hormone causes fragmentation of the Golgi apparatus and disrupts endoplasmic reticulum-to-Golgi traffic.
Graves T, Patel S, Dannies P, Hinkle P. Misfolded growth hormone causes fragmentation of the Golgi apparatus and disrupts endoplasmic reticulum-to-Golgi traffic. Journal Of Cell Science 2001, 114: 3685-94. PMID: 11707520, DOI: 10.1242/jcs.114.20.3685.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAnti-Bacterial AgentsBiomarkersCarrier ProteinsChromatinCoatomer ProteinCOS CellsEndoplasmic ReticulumEndoplasmic Reticulum Chaperone BiPGolgi ApparatusGreen Fluorescent ProteinsHeat-Shock ProteinsHuman Growth HormoneHumansIndicators and ReagentsLuminescent ProteinsMembrane ProteinsMicrotubule-Organizing CenterMicrotubulesMolecular ChaperonesProlactinProtein FoldingProtein TransportQb-SNARE ProteinsReceptors, Thyrotropin-Releasing HormoneTunicamycinConceptsWild-type growth hormoneUnfolded protein responseGolgi trafficEndoplasmic reticulumBeta-COPProtein responseGolgi apparatusWild-type human growth hormonePlasma membrane proteinsGolgi marker beta-COPMicrotubule-organizing centerAmino acids 32Thyrotropin-releasing hormone receptorGolgi fragmentationMembrane proteinsSubcellular localizationGolgi markersCOS7 cellsBiP mRNASecretory proteinsReceptor traffickingHost cellsMembrinMicrotubular arrangementTrafficking
2000
Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor
Lee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 2000, 141: 3485-3492. PMID: 10965922, DOI: 10.1210/endo.141.9.7647.Peer-Reviewed Original ResearchConceptsSecretory pathwayEpidermal growth factorSynaptotagmin IIICyclophilin BGH4C1 cellsInduction of proteinsGrowth factorGlucose-regulated protein 94Rat pituitary GH4C1 cellsSecretory granulesSynaptosomal-associated proteinMessenger RNA accumulationMembrane proteinsPituitary GH4C1 cellsSynaptosomal associated proteinRNA accumulationPlasma membraneProtein AssociatedSpecific proteinsSynaptotagmin IHormone-treated cellsSNAP-25Protein 94Secretory granule accumulationCoordinate event
1999
Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting*
Dannies P. Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting*. Endocrine Reviews 1999, 20: 3-21. PMID: 10047971, DOI: 10.1210/edrv.20.1.0354.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecretory granule proteinsMembrane proteinsGranule proteinsSecretory granule membrane proteinsPossible recognition siteGranule membrane proteinSecretory granule membranesThree-dimensional electron microscopyTransport vesiclesCell biologyConstitutive pathwayRegulated pathwayMembrane lipidsGranule formationGranule membranesSorting mechanismHormone aggregationProteinDense core granulesProcess of aggregationRecognition sitesSame cellsNeuroendocrine cellsSecretory granulesProtein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting
Dannies P. Protein Hormone Storage in Secretory Granules: Mechanisms for Concentration and Sorting. Endocrine Reviews 1999, 20: 3-21. DOI: 10.1210/er.20.1.3.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecretory granule proteinsMembrane proteinsGranule proteinsSecretory granule membrane proteinsPossible recognition siteGranule membrane proteinSecretory granule membranesThree-dimensional electron microscopyTransport vesiclesCell biologyConstitutive pathwayRegulated pathwayMembrane lipidsGranule formationGranule membranesSorting mechanismHormone aggregationProteinDense core granulesProcess of aggregationRecognition sitesSame cellsNeuroendocrine cellsSecretory granules