2000
Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor
Lee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor. Endocrinology 2000, 141: 3485-3492. PMID: 10965922, DOI: 10.1210/endo.141.9.7647.Peer-Reviewed Original ResearchConceptsSecretory pathwayEpidermal growth factorSynaptotagmin IIICyclophilin BGH4C1 cellsInduction of proteinsGrowth factorGlucose-regulated protein 94Rat pituitary GH4C1 cellsSecretory granulesSynaptosomal-associated proteinMessenger RNA accumulationMembrane proteinsPituitary GH4C1 cellsSynaptosomal associated proteinRNA accumulationPlasma membraneProtein AssociatedSpecific proteinsSynaptotagmin IHormone-treated cellsSNAP-25Protein 94Secretory granule accumulationCoordinate eventAccumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor*
Lee M, Zhu Y, Sun Z, Rhee H, Jeromin A, Roder J, Dannies P. Accumulation of Synaptosomal-Associated Protein of 25 kDa (SNAP-25) and Other Proteins Associated with the Secretory Pathway in GH4C1 Cells Upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor*. Endocrinology 2000, 141: 3485-3492. DOI: 10.1210/en.141.9.3485.Peer-Reviewed Original ResearchSNAP-25Secretory pathwaySynaptosomal-associated proteinSynaptotagmin IIIEpidermal growth factorCyclophilin BTreatment of GH4C1 cellsSecretory granulesSecretory granule accumulationGH4C1 cellsGlucose-regulated protein 94Rat pituitary GH4C1 cellsHormone-treated cellsInduction of proteinsPituitary GH4C1 cellsSynaptotagmin IRNA accumulationPlasma membraneSynaptotagminMessenger RNA accumulationInduced accumulationCell surfaceGranule accumulationProteinGrowth factor
1998
Stabilization of the Receptor Protein Tyrosine Phosphatase-Like Protein ICA512 in GH4C1 Cells upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor*
Lee M, Dirkx R, Solimena M, Dannies P. Stabilization of the Receptor Protein Tyrosine Phosphatase-Like Protein ICA512 in GH4C1 Cells upon Treatment with Estradiol, Insulin, and Epidermal Growth Factor*. Endocrinology 1998, 139: 2727-2733. DOI: 10.1210/en.139.6.2727.Peer-Reviewed Original ResearchSecretory granule membranesEpidermal growth factorGTP-binding protein Rab3Granule membranesSecretory granule accumulationSecretory granule compartmentAssociated with secretory granule membranesTyrosine phosphatase-like proteinPhosphatase-like proteinGH4C1 cellsProlactin storageGH4C1 rat pituitary tumor cellsIncreased transcriptionPulse-chase procedureMature formRat pituitary tumor cellsSecretory granulesGranule compartmentGranule accumulationTreated cellsGrowth factorUntreated cellsPituitary tumor cellsMessenger RNAICA512
1995
Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage
Mastro R, Dannies P. Lack of correlation of distribution of prolactin (PRL) charge isoforms with induction of PRL storage. Endocrinology 1995, 136: 69-74. PMID: 7828559, DOI: 10.1210/endo.136.1.7828559.Peer-Reviewed Original ResearchConceptsEpidermal growth factorNormal lactotrophsGH4C1 cellsGrowth factorForms of PRLFemale rat pituitary glandsNM epidermal growth factorRat pituitary glandDense core granulesRat pituitary tumor cellsPRL storagePituitary tumor cellsNM estradiolTotal PRLPituitary glandPRLTumor cellsCore granulesInsulinNM insulinLack of correlationLactotrophs
1994
Inhibition of rat prolactin (PRL) storage by coexpression of human PRL.
Arrandale J, Dannies P. Inhibition of rat prolactin (PRL) storage by coexpression of human PRL. Endocrinology 1994, 8: 1083-1090. PMID: 7997234, DOI: 10.1210/mend.8.8.7997234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAsparagineBiological TransportCell CompartmentationCytoplasmic GranulesEpidermal Growth FactorEstradiolGene ExpressionHumansInsulinModels, MolecularPituitary NeoplasmsProlactinProtein ConformationRatsRecombinant Fusion ProteinsSerineSpecies SpecificityTransfectionTumor Cells, CulturedConceptsHuman PRLRat PRLGH4C1 cellsReceptor-mediated mechanismNM epidermal growth factorSecretory granulesNM estradiolDense-core secretory granulesEpidermal growth factorHormone treatmentPRL storageProlactin storagePRLGrowth factorNM insulinMore ratsUntransfected cellsRatsRegulated pathwayControl culturesCellsInhibitionMarked specificity
1990
Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated.
Reaves B, Van Itallie C, Moore H, Dannies P. Prolactin and insulin are targeted to the regulated pathway in GH4C1 cells, but their storage is differentially regulated. Endocrinology 1990, 4: 1017-26. PMID: 2284004, DOI: 10.1210/mend-4-7-1017.Peer-Reviewed Original ResearchConceptsGH4C1 cellsEpidermal growth factorPRL synthesisGrowth factorPreferential increaseCombination of estradiolRegulated pathwayRat pituitary tumor cellsIntracellular PRLPituitary tumor cellsBasal releasePRL releaseIntracellular proinsulinSame time courseHormone treatmentPRL storageTumor cellsProinsulin immunoreactivityMRNA levelsPRLInsulinSame peak heightHormoneProinsulinTreatmentComparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line
Flicker L, Reaves B, Das B, Dannies P. Comparison of the Regulation of Carboxypeptidase E and Prolactin in GH4C1 Cells, a Rat Pituitary Cell Line. Neuroendocrinology 1990, 51: 658-663. PMID: 2114002, DOI: 10.1159/000125407.Peer-Reviewed Original ResearchConceptsGH4C1 cellsPituitary cell lineEpidermal growth factorNM thyrotropin-releasing hormoneCarboxypeptidase ECell linesCPE activityGrowth factorThyrotropin-releasing hormoneLevels of prolactinCPE mRNAAnterior pituitary cell lineIntracellular levelsSecretory granulesRat anterior pituitary cell lineRat pituitary cell lineGH4C1 cell lineProlactinControl valuesIntracellular prolactinTreatmentNorthern blot analysisBlot analysisEstradiolInsulin
1988
Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells.
Van Itallie C, Dannies P. Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells. Endocrinology 1988, 2: 332-7. PMID: 2837664, DOI: 10.1210/mend-2-4-332.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DivisionDNADNA, RecombinantElectron Transport Complex IVEnzyme InductionEpidermal Growth FactorEstradiolFemaleInsulinMaleMitochondriaMolecular Sequence DataNucleic Acid HybridizationPituitary NeoplasmsProlactinRatsRats, Inbred StrainsRNA, MessengerTumor Cells, CulturedConceptsCytochrome oxidase II geneCytochrome oxidaseMitochondrial ribonucleic acidMitochondrial genomePituitary tumor cell lineEpidermal growth factorMRNA accumulationNumber of copiesTumor cell linesPituitary tumor cellsCell proliferationMRNACell linesRibonucleic acidMaximal inductionGenesGrowth factorGrowth ratePRL mRNA
1986
Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*
SCAMMELL J, BURRAGE T, DANNIES P. Hormonal Induction of Secretory Granules in a Pituitary Tumor Cell Line*. Endocrinology 1986, 119: 1543-1548. PMID: 3530721, DOI: 10.1210/endo-119-4-1543.Peer-Reviewed Original ResearchConceptsGH4C1 cellsIntracellular PRLSecretory granulesCombination of estradiolNumber of granulesPituitary tumor cell linePRL accumulationHormone regimenTumor cell strainsEpidermal growth factorHormone treatmentTumor cell linesPRLGrowth factorControl levelsOccasional granulesCell linesHormonal inductionGHCell strainsCellsGranule numberTreatmentCellular contentStorage granules
1982
Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells.
Kiino D, Dannies P. Hormonal regulation of prolactin storage in a clonal strain of rat pituitary tumor cells. The Yale Journal Of Biology And Medicine 1982, 55: 409-20. PMID: 6763814, PMCID: PMC2596570.Peer-Reviewed Original ResearchConceptsProlactin storageRat pituitary tumor cellsGH cellsPituitary tumor cellsPituitary functionNM estradiolTumor cellsProlactin synthesisEstradiol-induced increaseCells/NM insulinDays of treatmentProlactin-secreting cellsCell cycle distributionPercent increaseClonal strainsHormone secretionEpidermal growth factorPotassium depolarizationProlactin productionEstradiolHormone productionProlactinInsulinIntracellular prolactin