1997
STEP: a family of brain-enriched PTPs. Alternative splicing produces transmembrane, cytosolic and truncated isoforms.
Bult A, Zhao F, Dirkx R, Raghunathan A, Solimena M, Lombroso P. STEP: a family of brain-enriched PTPs. Alternative splicing produces transmembrane, cytosolic and truncated isoforms. European Journal Of Cell Biology 1997, 72: 337-44. PMID: 9127733.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernBlotting, WesternBrainCalcium-Binding ProteinsCalnexinCHO CellsCricetinaeFemaleFluorescent Antibody Technique, IndirectMembrane ProteinsMolecular Sequence DataProtein Tyrosine PhosphatasesProtein Tyrosine Phosphatases, Non-ReceptorRatsSynaptophysinTransfectionConceptsProtein tyrosine phosphataseCatalytic phosphatase domainProtein tyrosine kinase familyHydrophobic amino acid sequenceAlternative splicing mechanismAmino acid sequencePrevious biochemical studiesTyrosine kinase familyStop codon upstreamPhosphatase domainCytosolic variantAlternative splicingMembrane compartmentsTyrosine phosphataseKinase familySplicing mechanismSubcellular localizationCytosolic proteinsAcid sequenceN-terminusInactive variantContinuous sucrose gradientSTEP isoformsPolyproline domainEndoplasmic reticulum
1996
STEP61: A Member of a Family of Brain-Enriched PTPs Is Localized to the Endoplasmic Reticulum
Bult A, Zhao F, Dirkx R, Sharma E, Lukacsi E, Solimena M, Naegele J, Lombroso P. STEP61: A Member of a Family of Brain-Enriched PTPs Is Localized to the Endoplasmic Reticulum. Journal Of Neuroscience 1996, 16: 7821-7831. PMID: 8987810, PMCID: PMC6579237, DOI: 10.1523/jneurosci.16-24-07821.1996.Peer-Reviewed Original ResearchConceptsNovel N-terminal regionEnzymatic activityEndoplasmic reticulumPutative transmembrane domainsIntrinsic membrane proteinsExtended N-terminusAmino acid sequenceN-terminal regionDistinctive intracellular distributionsProtein tyrosineCytosolic variantTransmembrane domainMembrane compartmentsMembrane proteinsAcid sequenceSubcellular fractionationN-terminusRecombinant proteinsTransfection experimentsSTEP61Intracellular distributionProteinReticulumImmunocytochemical labelingFamilyTransient compartmental expression of a family of protein tyrosine phosphatases in the developing striatum
Raghunathan A, Matthews G, Lombroso P, Naegele J. Transient compartmental expression of a family of protein tyrosine phosphatases in the developing striatum. Brain Research 1996, 91: 190-199. PMID: 8852369, DOI: 10.1016/0165-3806(95)00176-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernBlotting, WesternCalbindinsCell CountDopamine and cAMP-Regulated Phosphoprotein 32Enzyme InhibitorsFemaleGene Expression Regulation, DevelopmentalIsoenzymesNeostriatumNerve Tissue ProteinsNeuronsPhosphoproteinsPostpartum PeriodPregnancyProtein Tyrosine PhosphatasesRatsRats, Inbred StrainsS100 Calcium Binding Protein GSubstance PSubstantia NigraTime FactorsTranscription, GeneticConceptsPatch neuronsPostnatal weekTwo-color immunofluorescent stainingTyrosine hydroxylase-immunoreactive fibersDopaminoceptive brain regionsCalbindin-positive neuronsHydroxylase-immunoreactive fibersStriatum of ratsDopamine islandsPostnatal day 1Most striatal neuronsFirst postnatal weekEarly postnatal developmentMatrix neuronsStriatal afferentsStriatal neuronsImmunoreactive fibersSubstance PLateral striatumDay 1StriatumPostnatal developmentBrain regionsNeuronsCompartmental expression