Featured Publications
Embryology with precision: effective quality control in the in vitro fertilization laboratory
Basar M, Unsal E, Ergun Y. Embryology with precision: effective quality control in the in vitro fertilization laboratory. Current Opinion In Obstetrics & Gynecology 2024, 36: 200-207. PMID: 38572581, DOI: 10.1097/gco.0000000000000945.Peer-Reviewed Original ResearchConceptsPreimplantation genetic testingIn vitro fertilizationIntracytoplasmic sperm injectionProportion of mature oocytesIn vitro fertilization treatmentClinical pregnancy rateClinical practiceIn vitro fertilization laboratoryEmbryo development ratesImprove IVF outcomesSuccess rateImproving clinical outcomesField of reproductive medicineSperm injectionIVF outcomesClinical outcomesIVF treatmentDiagnostic accuracySuccess rate of IVF treatmentIVF proceduresPregnancy rateGenetic testingMature oocytesReproductive medicineGenetics laboratories
2024
Beyond Earth’s bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space
Chaplia O, Mathyk B, Nichols-Burns S, Basar M, Halicigil C. Beyond Earth’s bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space. Reproductive Biology And Endocrinology 2024, 22: 123. PMID: 39394617, PMCID: PMC11468284, DOI: 10.1186/s12958-024-01290-y.Peer-Reviewed Original ResearchConceptsAssisted reproductive technologyIn vitro embryo developmentReproductive technologyRadiation-induced damageGamete handlingClinical embryologistsReproductive endocrinologistsTemperature limitEmbryo cultureReproductive medicineLow Earth orbitEmbryo developmentDeep space travelEarth orbitSpace environmentInterplanetary speciesEarth-boundMicrofluidic devicesCryopreservationMitochondrial Unfolded Protein Response Gene Clpp Is Required for Oocyte Function and Female Fertility
Ergun Y, Imamoglu A, Cozzolino M, Demirkiran C, Basar M, Garg A, Yildirim R, Seli E. Mitochondrial Unfolded Protein Response Gene Clpp Is Required for Oocyte Function and Female Fertility. International Journal Of Molecular Sciences 2024, 25: 1866. PMID: 38339144, PMCID: PMC10855406, DOI: 10.3390/ijms25031866.Peer-Reviewed Original ResearchConceptsCaseinolytic peptidase PMouse modelProtein homeostasisStress responseUnfolded protein stress responseProtein stress responseCumulus/granulosa cellsOocyte competenceOocyte functionGlobal deletionFunctional abnormalitiesGenes clpPMetabolic stress responseFemale subfertilityFemale infertilityOocyte-specificOocytesReproductive functionMtUPRMiceProtein degradationReproductive competenceFemale fertilityDeletionHomeostasis
2022
Noninvasive amino acid turnover predicts human embryo aneuploidy
Olcay IO, Akcay B, Bahceci M, Arici A, Boynukalin K, Yakicier C, Ozpinar A, Basar M. Noninvasive amino acid turnover predicts human embryo aneuploidy. Gynecological Endocrinology 2022, 38: 461-466. PMID: 35481385, DOI: 10.1080/09513590.2022.2068520.Peer-Reviewed Original ResearchConceptsEuploid groupAneuploid groupAmino acid turnoverAneuploid embryosHigher implantation rateAmino acid metabolism profilePotential diagnostic testPreimplantation genetic testingAcid turnoverBlank control groupEmbryo selection criteriaAssisted reproduction technologyMultiple pregnanciesImplantation ratePatient groupAmino acid concentrationsAneuploid blastocystsEmbryo aneuploidyControl groupDay 5IVF clinicsLow success rateGenetic testingCulture mediumTE biopsy
2015
Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding
Kayisli O, Kayisli UA, Basar M, Semerci N, Schatz F, Lockwood CJ. Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding. PLOS ONE 2015, 10: e0137855. PMID: 26436918, PMCID: PMC4593551, DOI: 10.1371/journal.pone.0137855.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsMedroxyprogesterone acetateGuinea pigsUterine bleedingCultured human endometrial stromal cellsStromal cellsGlandular cellsProgestin only contraceptivesEndometrial blood flowOvariectomized guinea pigsMajor side effectsEffective family planning methodsGlucocorticoid receptor activationGR transcriptional activityFragile blood vesselsFamily planning methodsIngenuity Pathway AnalysisFKBP51 expressionFunctional withdrawalMicroarray resultsOnly contraceptivesGlucocorticoid withdrawalEndometrial inflammationMechanisms of chorioamnionitis‐associated preterm birth: interleukin‐1β inhibits progesterone receptor expression in decidual cells
Guzeloglu-Kayisli O, Kayisli UA, Semerci N, Basar M, Buchwalder LF, Buhimschi CS, Buhimschi IA, Arcuri F, Larsen K, Huang JS, Schatz F, Lockwood CJ. Mechanisms of chorioamnionitis‐associated preterm birth: interleukin‐1β inhibits progesterone receptor expression in decidual cells. The Journal Of Pathology 2015, 237: 423-434. PMID: 26175191, DOI: 10.1002/path.4589.Peer-Reviewed Original ResearchConceptsProgesterone receptor expressionPreterm birthDecidual cellsPR expressionIL-1βDC culturesReceptor expressionPR levelsIL-1β suppressionLower PR levelsFunctional progesterone withdrawalCOX-2 expressionRelease of cytokinesIL-1β treatmentDecidual prostaglandin productionP38 MAPK signaling cascadesCervical ripeningProgesterone withdrawalCOX inhibitorsImmunohistochemical stainingChorioamnionitisFetal membrane ruptureNF-kBProstaglandin productionPGF2α productionEnhanced Human Decidual Cell–Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal
Schatz F, Guzeloglu-Kayisli O, Basar M, Buchwalder LF, Ocak N, Guzel E, Guller S, Semerci N, Kayisli UA, Lockwood CJ. Enhanced Human Decidual Cell–Expressed FKBP51 May Promote Labor-Related Functional Progesterone Withdrawal. American Journal Of Pathology 2015, 185: 2402-2411. PMID: 26207680, DOI: 10.1016/j.ajpath.2015.05.014.Peer-Reviewed Original ResearchConceptsTerm decidual cellsDecidual cellsProgesterone receptorP4 withdrawalInterstitial trophoblastMedroxyprogesterone acetateDC culturesHuman term labourLow progesterone receptorsGlucocorticoid receptor expressionFunctional progesterone withdrawalCultured decidual cellsHuman decidual cellsPlasma progesterone levelsThird trimester trophoblastDC nucleiDecidual sectionsOrganon 2058Plasma P4 levelsTerm laborPR expressionProgesterone withdrawalProgesterone levelsReceptor expressionGR expressionEffects of tibolone and its metabolites on prolactin and insulin-like growth factor binding protein-1 expression in human endometrial stromal cells
Guzel E, Buchwalder L, Basar M, Kayisli U, Ocak N, Bozkurt I, Lockwood CJ, Schatz F. Effects of tibolone and its metabolites on prolactin and insulin-like growth factor binding protein-1 expression in human endometrial stromal cells. Gynecological Endocrinology 2015, 31: 414-418. PMID: 25856298, DOI: 10.3109/09513590.2015.1014788.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternContraceptive Agents, FemaleDexamethasoneEndometriumEnzyme-Linked Immunosorbent AssayEstradiolEstrenesEstrogen Receptor ModulatorsEstrogensFemaleFuransGlucocorticoidsHormone AntagonistsHumansInsulin-Like Growth Factor Binding Protein 1Medroxyprogesterone AcetateMifepristoneNorpregnanesNorpregnenesProlactinReal-Time Polymerase Chain ReactionRNA, MessengerStromal CellsConceptsHuman endometrial stromal cellsCultured human endometrial stromal cellsInsulin-like growth factorEndometrial stromal cellsIGFBP-1RT/PCRTibolone administrationStromal cellsGrowth factorEffects of tiboloneProlactin expressionProtein-1 expressionEndometrial atrophyTibolone metabolitesWestern blot analysisEndometrial bleedingMedroxyprogesterone acetateProlactin levelsTiboloneSpecific ELISAChronic effectsProlactin outputProtein 1ELISA resultsBlot analysisLong-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival
Kayisli UA, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson HC, Shapiro J, Summerfield T, Huang SJ, Prelle K, Schatz F, Lockwood CJ. Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 5153-5158. PMID: 25847994, PMCID: PMC4413279, DOI: 10.1073/pnas.1424814112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CountCell DifferentiationCell MovementCell ProliferationCell SurvivalCells, CulturedChemokine CCL2Contraceptive Agents, FemaleDesogestrelEndometriumFemaleGene Expression ProfilingGene Expression RegulationGuinea PigsHumansMedroxyprogesterone AcetateModels, BiologicalMuscle, Smooth, VascularMyocytes, Smooth MuscleOvariectomyProgestinsProliferating Cell Nuclear AntigenSignal TransductionSTAT1 Transcription FactorConceptsVascular smooth muscle cellsChemokine ligand 2Medroxyprogesterone acetateEndometrial vasculatureVSMC proliferationCultured vascular smooth muscle cellsSmooth muscle cellsSmooth muscle cell survivalVascular smooth muscle cell survivalCell nuclear antigenFragile microvesselsΑSMA immunoreactivityEndometrial vesselsΑSMA stainingPericyte proliferationSTAT1 inhibitorGuinea pigsMuscle cellsMuscle cell survivalCCL2 effectsEtonogestrelMigration assaysNuclear antigenVSMC differentiationWomenChinese herbal medicine for miscarriage affects decidual micro-environment and fetal growth
Piao L, Chen C, Yeh C, Basar M, Masch R, Cheng Y, Lockwood CJ, Schatz F, Huang SJ. Chinese herbal medicine for miscarriage affects decidual micro-environment and fetal growth. Placenta 2015, 36: 559-566. PMID: 25771406, PMCID: PMC4395550, DOI: 10.1016/j.placenta.2015.02.006.Peer-Reviewed Original ResearchConceptsFirst trimester decidual cellsIL-1βDecidual cellsPlacental weightFetal growthFood intakeResorption rateFetal resorption rateMaternal food intakeM-CSFC motif ligand 2Intrauterine growth restrictionIGF-IR expressionInducible protein-10Anti-CD163 antibodyQRT-PCRChinese herbal formulaIL-8 productionChinese herbal medicineG-CSF expressionEndothelial cell angiogenesisPlacental IGFDecidual macrophagesFetal weightCytokine expression
2014
Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells
Shapiro JP, Basar M, Kayisli UA, Guzeloglu-Kayisli O, Huang S, Suarez AA, Ozer HG, Schatz F, Lockwood CJ. Mass spectrometry identification of potential mediators of progestin-only contraceptive-induced abnormal uterine bleeding in human endometrial stromal cells. Contraception 2014, 91: 253-260. PMID: 25529278, PMCID: PMC4395468, DOI: 10.1016/j.contraception.2014.11.005.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsUterine bleedingStanniocalcin-1Cultured human endometrial stromal cellsStromal cellsMicroarray analysisEndometrial biopsyTubal metaplasiaMRNA microarray analysisSide effectsLAPCComplex biological responseEtonogestrelHypoxia treatmentProgestinsHypoxiaPrimary culturesPotential mediatorsPotential targetProtein mediatorsBleedingExpression levelsMedium supernatantInterferon-γ Protects First-Trimester Decidual Cells against Aberrant Matrix Metalloproteinases 1, 3, and 9 Expression in Preeclampsia
Lockwood CJ, Basar M, Kayisli UA, Guzeloglu-Kayisli O, Murk W, Wang J, De Paz N, Shapiro JP, Masch RJ, Semerci N, Huang SJ, Schatz F. Interferon-γ Protects First-Trimester Decidual Cells against Aberrant Matrix Metalloproteinases 1, 3, and 9 Expression in Preeclampsia. American Journal Of Pathology 2014, 184: 2549-2559. PMID: 25065683, PMCID: PMC4188280, DOI: 10.1016/j.ajpath.2014.05.025.Peer-Reviewed Original ResearchMeSH KeywordsBlotting, WesternDeciduaEnzyme-Linked Immunosorbent AssayFemaleHumansImmunohistochemistryInterferon-gammaKiller Cells, NaturalMacrophagesMatrix Metalloproteinase 1Matrix Metalloproteinase 3Matrix Metalloproteinase 9Matrix Metalloproteinases, SecretedPre-EclampsiaPregnancyPregnancy Trimester, FirstReal-Time Polymerase Chain ReactionConceptsMitogen-activated protein kinaseP38 mitogen-activated protein kinaseMitogen-activated protein kinase phosphorylationP38 mitogen-activated protein kinase phosphorylationExtracellular matrix proteinsProtein kinase phosphorylationHuman first trimester decidual cellsProtein kinaseReal-time quantitative RT-PCREVT invasionKinase phosphorylationQuantitative RT-PCRMatrix proteinsProteolytic cascadeDecidual cellsIntegrin receptorsExtravillous trophoblastsHuman extravillous trophoblastsIncomplete spiral arteryMMP-9Protein levelsProtein expressionMMP-1MMP-2Fibrillar collagenLong-Acting Progestin-Only Contraceptives Enhance Human Endometrial Stromal Cell Expressed Neuronal Pentraxin-1 and Reactive Oxygen Species to Promote Endothelial Cell Apoptosis
Guzeloglu-Kayisli O, Basar M, Shapiro J, Semerci N, Huang J, Schatz F, Lockwood C, Kayisli U. Long-Acting Progestin-Only Contraceptives Enhance Human Endometrial Stromal Cell Expressed Neuronal Pentraxin-1 and Reactive Oxygen Species to Promote Endothelial Cell Apoptosis. The Journal Of Clinical Endocrinology & Metabolism 2014, 99: e1957-e1966. PMID: 25029423, PMCID: PMC4184079, DOI: 10.1210/jc.2014-1770.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCaspase 3Cell ProliferationCells, CulturedContraceptive Agents, FemaleC-Reactive ProteinCulture Media, ConditionedCytochromes cEndometriumEndothelial CellsEstradiolFemaleHumansMedroxyprogesterone AcetateMicrovesselsNerve Tissue ProteinsParacrine CommunicationProgestinsReactive Oxygen SpeciesStromal CellsConceptsHuman endometrial endothelial cellsNeuronal pentraxin 1Abnormal uterine bleedingUterine bleedingMedroxyprogesterone acetateReactive oxygen species generationCultured human endometrial endothelial cellsOxygen species generationHuman endometrial stromal cellsUterine blood flowEndometrium of womenEndometrial endothelial cellsEndometrial stromal cellsProgesterone receptor proteinCaspase-3Elevated reactive oxygen species (ROS) generationHESC-CMsNovel paracrine mechanismEndothelial cell apoptosisSpecies generationFragile microvesselsCytosolic cytochrome c levelsParacrine mechanismsBlood flowLAPC
2012
Statins Inhibit Monocyte Chemotactic Protein 1 Expression in Endometriosis
Cakmak H, Basar M, Seval-Celik Y, Osteen KG, Duleba AJ, Taylor HS, Lockwood CJ, Arici A. Statins Inhibit Monocyte Chemotactic Protein 1 Expression in Endometriosis. Reproductive Sciences 2012, 19: 572-579. PMID: 22267540, PMCID: PMC3439122, DOI: 10.1177/1933719111430998.Peer-Reviewed Original ResearchConceptsCultured endometriotic cellsMonocyte chemotactic protein-1 expressionChemotactic protein-1 expressionMCP-1 productionEndometriotic cellsProtein-1 expressionEndometriotic implantsMouse modelMCP-1 messenger RNA expressionExerts anti-inflammatory effectsAnti-inflammatory effectsEffect of statinsAnti-inflammatory propertiesMCP-1 expressionMevalonate pathwayDose-dependent inhibitionNude mouse modelMessenger RNA expressionDose-dependent mannerEctopic endometriumEndometrial cellsPeritoneal surfaceStatinsPotential treatmentInhibitory effect
2011
Bidirectional Interaction Between Unfolded-Protein-Response Key Protein HSPA5 and Estrogen Signaling in Human Endometrium1
Guzel E, Basar M, Ocak N, Arici A, Kayisli UA. Bidirectional Interaction Between Unfolded-Protein-Response Key Protein HSPA5 and Estrogen Signaling in Human Endometrium1. Biology Of Reproduction 2011, 85: 121-127. PMID: 21389343, DOI: 10.1095/biolreprod.110.089532.Peer-Reviewed Original ResearchConceptsHSPA5 expressionHuman endometriumMenstrual cycleStromal cellsUnfolded protein responseNormal human endometriumEndometrial glandularEstradiol levelsHuman Endometrium1Possible inverse correlationIshikawa cellsEstrogen signalingLocal paracrineEndometriumSteroid hormonesAutocrine factorHeat shock 70EstrogenInverse correlationBidirectional regulationExpression levelsBidirectional interactionsShock 70Dynamic tissueHSPA5
2010
The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia
Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ. The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia. American Journal Of Pathology 2010, 177: 2472-2482. PMID: 20829438, PMCID: PMC2966804, DOI: 10.2353/ajpath.2010.091247.Peer-Reviewed Original ResearchConceptsGranulocyte-macrophage colony-stimulating factorColony-stimulating factorPathogenesis of preeclampsiaDendritic cellsDecidual cellsFirst trimester human decidual cellsGranulocyte-macrophage colony-stimulating factor (GM-CSF) expressionGenesis of preeclampsiaSystemic inflammatory stateInterleukin-1 βTumor necrosis factorHuman decidual cellsColony-stimulating factor expressionFactor expression levelsColony-stimulating factor productionGM-CSF expressionGranulocyte-macrophage colony-stimulating factor productionPreeclamptic deciduaShallow placentationInflammatory stateHuman deciduaNecrosis factorMurine modelPreeclampsiaMouse modelPreeclampsia-related increase of interleukin-11 expression in human decidual cells
Basar M, Yen CF, Buchwalder LF, Murk W, Huang SJ, Godlewski K, Kocamaz E, Arda O, Schatz F, Lockwood CJ, Kayisli UA. Preeclampsia-related increase of interleukin-11 expression in human decidual cells. Reproduction 2010, 140: 605-612. PMID: 20668109, PMCID: PMC3795512, DOI: 10.1530/rep-10-0263.Peer-Reviewed Original ResearchMeSH KeywordsAdultDeciduaEstradiolFemaleHumansImidazolesImmunohistochemistryInterleukin-11Interleukin-1betaNaphthalenesNF-kappa BP38 Mitogen-Activated Protein KinasesPre-EclampsiaPregnancyProtein Kinase CPyridinesReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStatistics, NonparametricTumor Necrosis Factor-alphaConceptsFirst trimester decidual cellsDecidual cellsPlacental sectionsLeukocyte-free first trimester decidual cellsGestational age-matched controlsProtein kinase CMaternal-placental interfaceUteroplacental blood flowPathogenesis of preeclampsiaAnti-inflammatory processesCultured decidual cellsTumor necrosis factorAge-matched controlsHuman decidual cellsQuantitative RT-PCRSystemic inflammationNecrosis factorPreeclampsiaBlood flowP38 MAP kinaseIL11 expressionNuclear factorStimulatory effectIL11RT-PCR
2008
Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells
Lockwood CJ, Yen CF, Basar M, Kayisli UA, Martel M, Buhimschi I, Buhimschi C, Huang SJ, Krikun G, Schatz F. Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells. American Journal Of Pathology 2008, 172: 1571-1579. PMID: 18467705, PMCID: PMC2408417, DOI: 10.2353/ajpath.2008.070629.Peer-Reviewed Original ResearchConceptsIL-6 levelsIL-6Decidual cellsGestational age-matched control placentasElevated IL-6 levelsIL-6 mRNA levelsCommon pregnancy disorderGenesis of preeclampsiaUteroplacental blood flowFetal-placental unitInterleukin-6 levelsPathogenesis of preeclampsiaPro-inflammatory cytokinesEndothelial cell dysfunctionInterleukin-6 expressionHuman decidual cellsReal-time quantitative RT-PCRTime quantitative RT-PCRQuantitative RT-PCRFetal morbidityDecidual macrophagesPreeclamptic womenSystemic inflammationPregnancy disordersControl placentas
2007
Basic aspects of implantation
Guzeloglu-Kayisli O, Basar M, Arici A. Basic aspects of implantation. Reproductive BioMedicine Online 2007, 15: 728-739. PMID: 18062873, DOI: 10.1016/s1472-6483(10)60541-x.Peer-Reviewed Original ResearchConceptsSuccessful implantationPlacental formationGp130 cytokine familyIL-15 systemNumber of cytokinesGrowth factor-beta superfamilyColony-Stimulating FactorLeukemia inhibitory factorProcess of implantationSynchronized dialogueUterine factorsReceptive endometriumUterine growthIL-1Sex steroidsEmbryo implantationBlastocyst adhesionInhibitory factorCytokine familyImplantation sitesGrowth factorJuxtacrine mannerImplantationSpecific cohortCytokines
2006
Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells
Selam B, Kayisli UA, Akbas GE, Basar M, Arici A. Regulation of FAS Ligand Expression by Chemokine Ligand 2 in Human Endometrial Cells. Biology Of Reproduction 2006, 75: 203-209. PMID: 16687653, DOI: 10.1095/biolreprod.105.045716.Peer-Reviewed Original ResearchConceptsFASLG expressionEndometrial stromal cellsStromal cellsChemokine ligand 2Protein expressionSemiquantitative RT-PCR analysisRT-PCR analysisFAS ligand expressionHuman endometriumCellular growthWestern blot analysisRate of apoptosisCultured endometrial stromal cellsExtracellular matrixJurkat cellsIncreased apoptotic rateHuman endometrial stromal cellsApoptotic moleculesLevels of CCL2Blot analysisDynamic tissueNumerous hormonesApoptosisHuman endometrial cellsSame cells