2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
2002
Hydrolyzable ATP and PIP2 Modulate the Small-conductance K+ Channel in Apical Membranes of Rat Cortical-Collecting Duct (CCD)
Lu M, Hebert SC, Giebisch G. Hydrolyzable ATP and PIP2 Modulate the Small-conductance K+ Channel in Apical Membranes of Rat Cortical-Collecting Duct (CCD). The Journal Of General Physiology 2002, 120: 603-615. PMID: 12407074, PMCID: PMC2229550, DOI: 10.1085/jgp.20028677.Peer-Reviewed Original ResearchMeSH Keywords1-Phosphatidylinositol 4-KinaseAdenosine TriphosphateAnimalsCell MembraneCyclic AMP-Dependent Protein KinasesElectrophysiologyHydrolysisKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsPatch-Clamp TechniquesPhosphatidylinositol 4,5-DiphosphatePhosphorylationPotassium ChannelsPotassium Channels, Inwardly RectifyingProtein Interaction MappingRatsRats, Sprague-DawleyAbsence of Small Conductance K+ Channel (SK) Activity in Apical Membranes of Thick Ascending Limb and Cortical Collecting Duct in ROMK (Bartter's) Knockout Mice*
Lu M, Wang T, Yan Q, Yang X, Dong K, Knepper MA, Wang W, Giebisch G, Shull GE, Hebert SC. Absence of Small Conductance K+ Channel (SK) Activity in Apical Membranes of Thick Ascending Limb and Cortical Collecting Duct in ROMK (Bartter's) Knockout Mice*. Journal Of Biological Chemistry 2002, 277: 37881-37887. PMID: 12130653, PMCID: PMC4426997, DOI: 10.1074/jbc.m206644200.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsBartter SyndromeBase SequenceCell MembraneDisease Models, AnimalDNA PrimersGene Expression RegulationGenotypeHumansKidneyKidney CortexKidney Tubules, CollectingMiceMice, KnockoutPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium Channels, Inwardly RectifyingPotassium ChlorideSmall-Conductance Calcium-Activated Potassium ChannelsSurvival AnalysisConceptsThick ascending limbSK channel activityROMK null miceBartter's syndromeNull miceSK channelsAscending limbChannel activityExtracellular volume depletionROMK geneCortical collecting ductsWild-type littermatesAbsorption/secretionROMK knockout miceNull mice exhibitPatch-clamp analysisSmall conductanceSignificant hydronephrosisRenal morphologyVolume depletionKnockout miceMice exhibitSyndromeCollecting ductsNaCl reabsorption
1998
Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1997
Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL
Wang W, Lu M, Balazy M, Hebert S. Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL. American Journal Of Physiology 1997, 273: f421-f429. PMID: 9321915, DOI: 10.1152/ajprenal.1997.273.3.f421.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumEstrenesFluorescent DyesFura-2GadoliniumHydroxyeicosatetraenoic AcidsKidney CortexKidney MedullaKineticsLoop of HenleMembrane PotentialsPatch-Clamp TechniquesPhosphodiesterase InhibitorsPhospholipases APhospholipases A2Potassium ChannelsPyrrolidinonesRatsRats, Sprague-DawleyTime FactorsConceptsThick ascending limbMicroM Gd3Phospholipase A2Extracellular Ca2Rat thick ascending limbAddition of thapsigarginEffects of stimulationInflux of Ca2Channel activityPatch-clamp techniquePhospholipase CCell-attached patchesMicroM gadoliniumFura-2Ascending limbFluorescent sodium indicatorIntracellular Ca2Rat kidneyInhibitory effectAcetoxymethyl esterInhibitionStimulationCa2Signaling mechanismSodium indicatorNitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD
Lu M, Giebisch G, Wang W. Nitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD. American Journal Of Physiology 1997, 272: f498-f504. PMID: 9140051, DOI: 10.1152/ajprenal.1997.272.4.f498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic GMPElectric ConductivityKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsModels, BiologicalNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillamineRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodium ChannelsTime FactorsConceptsL-NAMESodium nitroprussideNitric oxideNitro-L-arginine methyl esterL-NAME-induced decreaseAction of NOL-NAME resultsS-nitroso-N-acetyl penicillamineEffect of NOEffect of SNAPWhole-cell recordingsMicroM sodium nitroprussidePatch-clamp techniqueExogenous NO donorD-NAMENO synthaseMicroM amilorideRat CCDCell recordingsNO donorControl valuesStimulatory effectIndirect actionDependent pathwayHyperpolarization
1996
Nitric oxide regulates the low-conductance K+ channel in basolateral membrane of cortical collecting duct
Lu M, Wang W. Nitric oxide regulates the low-conductance K+ channel in basolateral membrane of cortical collecting duct. American Journal Of Physiology 1996, 270: c1336-c1342. PMID: 8967433, DOI: 10.1152/ajpcell.1996.270.5.c1336.Peer-Reviewed Original ResearchConceptsNitric oxide synthaseCGMP-dependent pathwayL-NAMEL-NIOChannel activityL-arginine methyl esterExogenous nitric oxide donorOutward slope conductanceSlope conductanceNitric oxide donorPatch-clamp techniqueCell-attached patchesInward slope conductanceOxide synthaseBasolateral membraneSodium nitroprussideOxide donorRat kidneyNitric oxideIntermediate conductanceInhibitory effectEffect of NOTetraethylammonium chlorideMicroM NCyclic monophosphate