2001
Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD
Wei Y, Lu M, Wang W. Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD. American Journal Of Physiology - Cell Physiology 2001, 280: c920-c928. PMID: 11245609, DOI: 10.1152/ajpcell.2001.280.4.c920.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzylaminesBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesDose-Response Relationship, DrugEnzyme InhibitorsFemaleIon Channel GatingIonomycinIonophoresKidney Tubules, CollectingMaleMembrane PotentialsNaphthalenesNG-Nitroarginine Methyl EsterNitratesPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseSpecific Pathogen-Free OrganismsStrophanthidinSulfonamidesSuperoxidesConceptsNitro-L-arginine methyl esterIntracellular Ca2Inhibitory effectNM Ca2Nitric oxideChannel activityEffects of strophanthidinKN-93KN-62Protein kinase CCalphostin CEffect of inhibitionCalmodulin-dependent kinase IICell-attached patchesExtracellular Ca2Rat CCDKinase CMicroM ionomycinRat kidneyMechanism of Ca2High concentrationsStrophanthidinCa2Methyl esterKinase II
2000
Extracellular Atp Inhibits the Small-Conductance K Channel on the Apical Membrane of the Cortical Collecting Duct from Mouse Kidney
Lu M, MacGregor G, Wang W, Giebisch G. Extracellular Atp Inhibits the Small-Conductance K Channel on the Apical Membrane of the Cortical Collecting Duct from Mouse Kidney. The Journal Of General Physiology 2000, 116: 299-310. PMID: 10919872, PMCID: PMC2229488, DOI: 10.1085/jgp.116.2.299.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAffinity LabelsAlkaloidsAnimalsCarbazolesColforsinCyclic AMPCyclic AMP-Dependent Protein KinasesCyclic GMP-Dependent Protein KinasesEnzyme InhibitorsExtracellular SpaceIndolesIon Channel GatingKidney Tubules, CollectingMembrane PotentialsMiceMice, Inbred C57BLNaphthalenesNG-Nitroarginine Methyl EsterOkadaic AcidPatch-Clamp TechniquesPhosphoprotein PhosphatasesPhosphorylationPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedProtein KinasesRatsRats, Sprague-DawleyReceptors, PurinergicSmall-Conductance Calcium-Activated Potassium ChannelsThionucleotidesUridine TriphosphateConceptsApical membraneChannel activityProtein kinase AProtein kinase C.Protein phosphatasePurinergic receptor stimulationSmall-conductance potassium channelsOkadaic acidExtracellular ATP concentrationKinase AKinase C.Addition of ATPG proteinsRat homologuePhospholipase CPhosphatase activitySK activitySmall-conductance K channelsApical receptorsATP inhibitsSingle-channel analysisATPNucleotide sensitivityApical K channelsK channels
1998
Nitric oxide increases the activity of the apical 70-pS K+ channel in TAL of rat kidney
Lu M, Wang X, Wang W. Nitric oxide increases the activity of the apical 70-pS K+ channel in TAL of rat kidney. American Journal Of Physiology 1998, 274: f946-f950. PMID: 9612333, DOI: 10.1152/ajprenal.1998.274.5.f946.Peer-Reviewed Original ResearchConceptsThick ascending limbNitric oxide synthaseNitric oxideRat kidneyL-NAMEL-NAME-induced inhibitionL-arginine methyl esterChannel activityMedullary thick ascending limbEffect of NOEffect of SNAPMM L-argininePatch-clamp techniqueCGMP-dependent pathwayD-NAMECell-attached patchesAngiotensin IIMicroM SNAPNOS activityOxide synthaseL-arginineAscending limbHenle's loopNO donorCGMP concentration
1997
Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct
Lu M, Giebisch G, Wang W. Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct. The Journal Of General Physiology 1997, 110: 717-726. PMID: 9382898, PMCID: PMC2229403, DOI: 10.1085/jgp.110.6.717.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsAnti-Arrhythmia AgentsBiological TransportCalciumCyclic GMPDiureticsEnzyme InhibitorsKidney Tubules, CollectingNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillaminePotassiumPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumSodium ChannelsSpecific Pathogen-Free OrganismsConceptsEffects of amilorideIntracellular Ca2Cell-attached patchesControl valuesChannel activityRat cortical collecting ductsNO-cGMP dependent pathwayNitric oxide synthaseFree bath solutionCortical collecting ductsNa/H exchangerPatch-clamp techniqueAddition of SNAPMicroM benzamilOxide synthaseChannel blockersFura-2MicroM amilorideExtracellular Ca2Rat CCDRemoval of Ca2Clamp techniqueNeuronal formCollecting ductsRat kidney
1996
Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney.
Lu M, Zhu Y, Balazy M, Reddy K, Falck J, Wang W. Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1996, 108: 537-547. PMID: 8972391, PMCID: PMC2229337, DOI: 10.1085/jgp.108.6.537.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsCell MembraneCytochrome P-450 Enzyme InhibitorsDose-Response Relationship, DrugEnzyme InhibitorsFatty Acids, UnsaturatedHydroxyeicosatetraenoic AcidsIn Vitro TechniquesLoop of HenleNaphthalenesNG-Nitroarginine Methyl EsterPatch-Clamp TechniquesPenicillaminePotassium Channel BlockersPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumConceptsEffect of AIIThick ascending limbAngiotensin IIControl valuesInhibitory effectChannel activityAscending limbRat kidneyL-arginine methyl esterArachidonic acidStimulatory effectExogenous nitric oxide donorNM angiotensin IIMicroM NG-nitroNitric oxide synthaseMajor AA metaboliteNitric oxide donorMicroM calphostin CPatch-clamp techniqueP450-dependent pathwayL-NAMENG-nitroCell-attached patchesOxide synthaseP450 metabolitesProtein kinase C stimulates the small-conductance K+ channel in the basolateral membrane of the CCD
Lu M, Wang W. Protein kinase C stimulates the small-conductance K+ channel in the basolateral membrane of the CCD. American Journal Of Physiology 1996, 271: f1045-f1051. PMID: 8945999, DOI: 10.1152/ajprenal.1996.271.5.f1045.Peer-Reviewed Original ResearchConceptsProtein kinase CCalphostin COkadaic acidKinase CEffect of PKCEffect of okadaicStimulator of PKCChannel activityBasolateral membraneExogenous protein kinase CCell-attached patchesMicroM okadaic acidNM calphostin CNitric oxide synthasesProtein phosphataseMembrane-delimited actionDirect phosphorylationPKC effects