2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
1998
The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels
Ali S, Chen X, Lu M, Xu J, Lerea K, Hebert S, Wang W. The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10274-10278. PMID: 9707637, PMCID: PMC21498, DOI: 10.1073/pnas.95.17.10274.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCarrier ProteinsColforsinCyclic AMPCyclic AMP-Dependent Protein Kinase Type IICyclic AMP-Dependent Protein KinasesFemaleGTP-Binding ProteinsIn Vitro TechniquesKidneyOocytesPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Inwardly RectifyingRecombinant ProteinsXenopus laevisConceptsEffect of forskolinMicroM forskolinProtein kinase APatch-clamp techniqueKinase ACAMP-dependent pathwayKidney cortexNeuronal tissueForskolinLines of evidenceROMK channelsEffect of cAMPKidneyCAMP mimicsXenopus oocytesPresent studyType II protein kinase ASecretory channelsOocytesROMK1 channelsMicroMRIICAMPAddition of ATPMinReaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1996
Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney.
Lu M, Zhu Y, Balazy M, Reddy K, Falck J, Wang W. Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1996, 108: 537-547. PMID: 8972391, PMCID: PMC2229337, DOI: 10.1085/jgp.108.6.537.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsCell MembraneCytochrome P-450 Enzyme InhibitorsDose-Response Relationship, DrugEnzyme InhibitorsFatty Acids, UnsaturatedHydroxyeicosatetraenoic AcidsIn Vitro TechniquesLoop of HenleNaphthalenesNG-Nitroarginine Methyl EsterPatch-Clamp TechniquesPenicillaminePotassium Channel BlockersPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumConceptsEffect of AIIThick ascending limbAngiotensin IIControl valuesInhibitory effectChannel activityAscending limbRat kidneyL-arginine methyl esterArachidonic acidStimulatory effectExogenous nitric oxide donorNM angiotensin IIMicroM NG-nitroNitric oxide synthaseMajor AA metaboliteNitric oxide donorMicroM calphostin CPatch-clamp techniqueP450-dependent pathwayL-NAMENG-nitroCell-attached patchesOxide synthaseP450 metabolitesProtein kinase C stimulates the small-conductance K+ channel in the basolateral membrane of the CCD
Lu M, Wang W. Protein kinase C stimulates the small-conductance K+ channel in the basolateral membrane of the CCD. American Journal Of Physiology 1996, 271: f1045-f1051. PMID: 8945999, DOI: 10.1152/ajprenal.1996.271.5.f1045.Peer-Reviewed Original ResearchConceptsProtein kinase CCalphostin COkadaic acidKinase CEffect of PKCEffect of okadaicStimulator of PKCChannel activityBasolateral membraneExogenous protein kinase CCell-attached patchesMicroM okadaic acidNM calphostin CNitric oxide synthasesProtein phosphataseMembrane-delimited actionDirect phosphorylationPKC effectsCytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL
Wang W, Lu M, Hebert S. Cytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL. American Journal Of Physiology 1996, 271: c103-c111. PMID: 8760035, DOI: 10.1152/ajpcell.1996.271.1.c103.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCell MembraneCytochrome P-450 Enzyme SystemExtracellular SpaceFatty Acids, UnsaturatedIn Vitro TechniquesLoop of HenleNeomycinPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleyTetradecanoylphorbol AcetateConceptsThick ascending limbCell-attached patchesChannel activityProtein kinase CCytochrome PArachidonic acidControl valuesInhibitory effectPatch-clamp techniqueEffects of neomycinCalphostin CMicroM neomycinExtracellular Ca2Ascending limbAddition of phorbolRat kidneyChannel open probabilityTAL tubulesNM calphostin CInhibition of PKC