2018
Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells
Lu M, Li JR, Alvarez-Lugo L, Li Y, Yu S, Li X, Shi B, Chai TC. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. American Journal Of Physiology - Cell Physiology 2018, 314: c643-c653. PMID: 29466671, DOI: 10.1152/ajpcell.00339.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic AMP-Dependent Protein KinasesFemaleLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsLipopolysaccharide ReceptorsLipopolysaccharidesLymphocyte Antigen 96Membrane PotentialsMice, Inbred C57BLPatch-Clamp TechniquesPotassiumSignal TransductionToll-Like Receptor 4Urinary BladderUrinary Tract InfectionsUrotheliumConceptsBK channel activityBK channelsChannel activityUmbrella cellsUrinary tract infectionFemale C57BL6 micePotassium channel inhibitorsUropathogenic Escherichia coliProtein kinase ATract infectionsLPS effectsC57BL6 miceBladder urotheliumLamina propriaMin of exposureChannel inhibitorsBacterial infectionsLPS receptorUrothelial tissuesUrothelial cellsUrotheliumLipopolysaccharidePotassium channelsRT-PCRPKA blocker
2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
2006
CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney
Lu M, Leng Q, Egan ME, Caplan MJ, Boulpaep EL, Giebisch GH, Hebert SC. CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney. Journal Of Clinical Investigation 2006, 116: 797-807. PMID: 16470247, PMCID: PMC1361349, DOI: 10.1172/jci26961.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCurcuminCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorHydrogen-Ion ConcentrationKidneyMiceMice, Inbred C57BLMice, Inbred CFTRMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingXenopus laevisConceptsFunctional switchCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelATP sensitivityEffects of CFTRThick ascending limbPotential physiological rolePKA activityRenal K channelsCystic fibrosisPhysiological roleSecretory channelsK channelsRenal tubule epithelial cellsApical membraneCFTRDeltaF508 mutationDistal nephron segmentsCl- channelsK homeostasisTubule epithelial cellsEpithelial cellsTAL cellsPotassium channelsK handlingGlibenclamide sensitivity
2003
ROMK is required for expression of the 70-pS K channel in the thick ascending limb
Lu M, Wang T, Yan Q, Wang W, Giebisch G, Hebert SC. ROMK is required for expression of the 70-pS K channel in the thick ascending limb. American Journal Of Physiology. Renal Physiology 2003, 286: f490-f495. PMID: 14600033, DOI: 10.1152/ajprenal.00305.2003.Peer-Reviewed Original ResearchConceptsThick ascending limbBartter's syndromeK dietK channelsAscending limbChannel activityApical K channelsFunctional expressionHypokalemic alkalosisTAL cellsNull miceSK activityHeterozygous miceHeterogeneous disorderMicePotassium recyclingROMKFunction mutationsSyndromeCritical subunitApical conductanceSalt absorptionLimbDietExpression
2002
Hydrolyzable ATP and PIP2 Modulate the Small-conductance K+ Channel in Apical Membranes of Rat Cortical-Collecting Duct (CCD)
Lu M, Hebert SC, Giebisch G. Hydrolyzable ATP and PIP2 Modulate the Small-conductance K+ Channel in Apical Membranes of Rat Cortical-Collecting Duct (CCD). The Journal Of General Physiology 2002, 120: 603-615. PMID: 12407074, PMCID: PMC2229550, DOI: 10.1085/jgp.20028677.Peer-Reviewed Original ResearchMeSH Keywords1-Phosphatidylinositol 4-KinaseAdenosine TriphosphateAnimalsCell MembraneCyclic AMP-Dependent Protein KinasesElectrophysiologyHydrolysisKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsPatch-Clamp TechniquesPhosphatidylinositol 4,5-DiphosphatePhosphorylationPotassium ChannelsPotassium Channels, Inwardly RectifyingProtein Interaction MappingRatsRats, Sprague-Dawley
2000
Extracellular Atp Inhibits the Small-Conductance K Channel on the Apical Membrane of the Cortical Collecting Duct from Mouse Kidney
Lu M, MacGregor G, Wang W, Giebisch G. Extracellular Atp Inhibits the Small-Conductance K Channel on the Apical Membrane of the Cortical Collecting Duct from Mouse Kidney. The Journal Of General Physiology 2000, 116: 299-310. PMID: 10919872, PMCID: PMC2229488, DOI: 10.1085/jgp.116.2.299.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAffinity LabelsAlkaloidsAnimalsCarbazolesColforsinCyclic AMPCyclic AMP-Dependent Protein KinasesCyclic GMP-Dependent Protein KinasesEnzyme InhibitorsExtracellular SpaceIndolesIon Channel GatingKidney Tubules, CollectingMembrane PotentialsMiceMice, Inbred C57BLNaphthalenesNG-Nitroarginine Methyl EsterOkadaic AcidPatch-Clamp TechniquesPhosphoprotein PhosphatasesPhosphorylationPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedProtein KinasesRatsRats, Sprague-DawleyReceptors, PurinergicSmall-Conductance Calcium-Activated Potassium ChannelsThionucleotidesUridine TriphosphateConceptsApical membraneChannel activityProtein kinase AProtein kinase C.Protein phosphatasePurinergic receptor stimulationSmall-conductance potassium channelsOkadaic acidExtracellular ATP concentrationKinase AKinase C.Addition of ATPG proteinsRat homologuePhospholipase CPhosphatase activitySK activitySmall-conductance K channelsApical receptorsATP inhibitsSingle-channel analysisATPNucleotide sensitivityApical K channelsK channelsTwo Types of K+ Channels are Present in the Apical Membrane of the Thick Ascending Limb of the Mouse Kidney
Lu M, Wang W. Two Types of K+ Channels are Present in the Apical Membrane of the Thick Ascending Limb of the Mouse Kidney. Kidney & Blood Pressure Research 2000, 23: 75-82. PMID: 10765108, DOI: 10.1159/000025957.Peer-Reviewed Original ResearchAdenosine TriphosphateAnimalsBariumBiophysical PhenomenaBiophysicsCell MembraneCytosolElectrophysiologyIntermediate-Conductance Calcium-Activated Potassium ChannelsKidneyMembrane PotentialsMicePatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsPotassium Channels, Calcium-ActivatedSmall-Conductance Calcium-Activated Potassium ChannelsTetraethylammonium
1998
The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels
Ali S, Chen X, Lu M, Xu J, Lerea K, Hebert S, Wang W. The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10274-10278. PMID: 9707637, PMCID: PMC21498, DOI: 10.1073/pnas.95.17.10274.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCarrier ProteinsColforsinCyclic AMPCyclic AMP-Dependent Protein Kinase Type IICyclic AMP-Dependent Protein KinasesFemaleGTP-Binding ProteinsIn Vitro TechniquesKidneyOocytesPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Inwardly RectifyingRecombinant ProteinsXenopus laevisConceptsEffect of forskolinMicroM forskolinProtein kinase APatch-clamp techniqueKinase ACAMP-dependent pathwayKidney cortexNeuronal tissueForskolinLines of evidenceROMK channelsEffect of cAMPKidneyCAMP mimicsXenopus oocytesPresent studyType II protein kinase ASecretory channelsOocytesROMK1 channelsMicroMRIICAMPAddition of ATPMinReaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidneyNitric oxide increases the activity of the apical 70-pS K+ channel in TAL of rat kidney
Lu M, Wang X, Wang W. Nitric oxide increases the activity of the apical 70-pS K+ channel in TAL of rat kidney. American Journal Of Physiology 1998, 274: f946-f950. PMID: 9612333, DOI: 10.1152/ajprenal.1998.274.5.f946.Peer-Reviewed Original ResearchConceptsThick ascending limbNitric oxide synthaseNitric oxideRat kidneyL-NAMEL-NAME-induced inhibitionL-arginine methyl esterChannel activityMedullary thick ascending limbEffect of NOEffect of SNAPMM L-argininePatch-clamp techniqueCGMP-dependent pathwayD-NAMECell-attached patchesAngiotensin IIMicroM SNAPNOS activityOxide synthaseL-arginineAscending limbHenle's loopNO donorCGMP concentration
1997
Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct
Lu M, Giebisch G, Wang W. Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct. The Journal Of General Physiology 1997, 110: 717-726. PMID: 9382898, PMCID: PMC2229403, DOI: 10.1085/jgp.110.6.717.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsAnti-Arrhythmia AgentsBiological TransportCalciumCyclic GMPDiureticsEnzyme InhibitorsKidney Tubules, CollectingNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillaminePotassiumPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumSodium ChannelsSpecific Pathogen-Free OrganismsConceptsEffects of amilorideIntracellular Ca2Cell-attached patchesControl valuesChannel activityRat cortical collecting ductsNO-cGMP dependent pathwayNitric oxide synthaseFree bath solutionCortical collecting ductsNa/H exchangerPatch-clamp techniqueAddition of SNAPMicroM benzamilOxide synthaseChannel blockersFura-2MicroM amilorideExtracellular Ca2Rat CCDRemoval of Ca2Clamp techniqueNeuronal formCollecting ductsRat kidneyPhospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL
Wang W, Lu M, Balazy M, Hebert S. Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL. American Journal Of Physiology 1997, 273: f421-f429. PMID: 9321915, DOI: 10.1152/ajprenal.1997.273.3.f421.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumEstrenesFluorescent DyesFura-2GadoliniumHydroxyeicosatetraenoic AcidsKidney CortexKidney MedullaKineticsLoop of HenleMembrane PotentialsPatch-Clamp TechniquesPhosphodiesterase InhibitorsPhospholipases APhospholipases A2Potassium ChannelsPyrrolidinonesRatsRats, Sprague-DawleyTime FactorsConceptsThick ascending limbMicroM Gd3Phospholipase A2Extracellular Ca2Rat thick ascending limbAddition of thapsigarginEffects of stimulationInflux of Ca2Channel activityPatch-clamp techniquePhospholipase CCell-attached patchesMicroM gadoliniumFura-2Ascending limbFluorescent sodium indicatorIntracellular Ca2Rat kidneyInhibitory effectAcetoxymethyl esterInhibitionStimulationCa2Signaling mechanismSodium indicatorNitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD
Lu M, Giebisch G, Wang W. Nitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD. American Journal Of Physiology 1997, 272: f498-f504. PMID: 9140051, DOI: 10.1152/ajprenal.1997.272.4.f498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic GMPElectric ConductivityKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsModels, BiologicalNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillamineRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodium ChannelsTime FactorsConceptsL-NAMESodium nitroprussideNitric oxideNitro-L-arginine methyl esterL-NAME-induced decreaseAction of NOL-NAME resultsS-nitroso-N-acetyl penicillamineEffect of NOEffect of SNAPWhole-cell recordingsMicroM sodium nitroprussidePatch-clamp techniqueExogenous NO donorD-NAMENO synthaseMicroM amilorideRat CCDCell recordingsNO donorControl valuesStimulatory effectIndirect actionDependent pathwayHyperpolarization
1996
Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney.
Lu M, Zhu Y, Balazy M, Reddy K, Falck J, Wang W. Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1996, 108: 537-547. PMID: 8972391, PMCID: PMC2229337, DOI: 10.1085/jgp.108.6.537.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsCell MembraneCytochrome P-450 Enzyme InhibitorsDose-Response Relationship, DrugEnzyme InhibitorsFatty Acids, UnsaturatedHydroxyeicosatetraenoic AcidsIn Vitro TechniquesLoop of HenleNaphthalenesNG-Nitroarginine Methyl EsterPatch-Clamp TechniquesPenicillaminePotassium Channel BlockersPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumConceptsEffect of AIIThick ascending limbAngiotensin IIControl valuesInhibitory effectChannel activityAscending limbRat kidneyL-arginine methyl esterArachidonic acidStimulatory effectExogenous nitric oxide donorNM angiotensin IIMicroM NG-nitroNitric oxide synthaseMajor AA metaboliteNitric oxide donorMicroM calphostin CPatch-clamp techniqueP450-dependent pathwayL-NAMENG-nitroCell-attached patchesOxide synthaseP450 metabolitesProtein kinase C stimulates the small-conductance K+ channel in the basolateral membrane of the CCD
Lu M, Wang W. Protein kinase C stimulates the small-conductance K+ channel in the basolateral membrane of the CCD. American Journal Of Physiology 1996, 271: f1045-f1051. PMID: 8945999, DOI: 10.1152/ajprenal.1996.271.5.f1045.Peer-Reviewed Original ResearchConceptsProtein kinase CCalphostin COkadaic acidKinase CEffect of PKCEffect of okadaicStimulator of PKCChannel activityBasolateral membraneExogenous protein kinase CCell-attached patchesMicroM okadaic acidNM calphostin CNitric oxide synthasesProtein phosphataseMembrane-delimited actionDirect phosphorylationPKC effectsCytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL
Wang W, Lu M, Hebert S. Cytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL. American Journal Of Physiology 1996, 271: c103-c111. PMID: 8760035, DOI: 10.1152/ajpcell.1996.271.1.c103.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCell MembraneCytochrome P-450 Enzyme SystemExtracellular SpaceFatty Acids, UnsaturatedIn Vitro TechniquesLoop of HenleNeomycinPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleyTetradecanoylphorbol AcetateConceptsThick ascending limbCell-attached patchesChannel activityProtein kinase CCytochrome PArachidonic acidControl valuesInhibitory effectPatch-clamp techniqueEffects of neomycinCalphostin CMicroM neomycinExtracellular Ca2Ascending limbAddition of phorbolRat kidneyChannel open probabilityTAL tubulesNM calphostin CInhibition of PKC
1995
Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney.
Wang W, Lu M. Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1995, 106: 727-743. PMID: 8576704, PMCID: PMC2229275, DOI: 10.1085/jgp.106.4.727.Peer-Reviewed Original ResearchConceptsThick ascending limbEffect of AAMicroM arachidonic acidArachidonic acidInhibitory effectAscending limbChannel activityAction of AARat kidneyMedullary thick ascending limbCytochrome P450 metabolic pathwayCytochrome P450 metabolitesPatch-clamp techniqueCell-attached patchesMicroM indomethacinP450 metabolitesMicroM linoleic acidStimulatory effectMetabolic pathwaysMain metaboliteKidneySpecific inhibitorLinoleic acidPredominant typeLimb