2019
Bladder urothelial BK channel activity is a critical mediator for innate immune response in urinary tract infection pathogenesis
Yeh J, Lu M, Alvarez-Lugo L, Chai TC. Bladder urothelial BK channel activity is a critical mediator for innate immune response in urinary tract infection pathogenesis. American Journal Of Physiology. Renal Physiology 2019, 316: f617-f623. PMID: 30648906, DOI: 10.1152/ajprenal.00554.2018.Peer-Reviewed Original ResearchConceptsUrinary tract infectionInnate immune responseImmune responseBK channel activityUrinary tract infection pathogenesisChannel activityFemale C57BL/6 miceInflammatory protein-2Voltage-gated potassium channelsUropathogenic Escherichia coliInhibitor iberiotoxinUPEC exposureUTI pathogenesisInflammatory biomarkersTract infectionsC57BL/6 miceIL-6Urinary biomarkersUrinary changesBiomarker levelsInfection pathogenesisUrine specimensProtein 10LPS inoculationSame biomarkers
2018
Mouse urothelial genes associated with voiding behavior changes after ovariectomy and bladder lipopolysaccharide exposure
Acevedo‐Alvarez M, Yeh J, Alvarez‐Lugo L, Lu M, Sukumar N, Hill WG, Chai TC. Mouse urothelial genes associated with voiding behavior changes after ovariectomy and bladder lipopolysaccharide exposure. Neurourology And Urodynamics 2018, 37: 2398-2405. PMID: 29682797, DOI: 10.1002/nau.23592.Peer-Reviewed Original ResearchConceptsUrinary tract infectionOveractive bladderOVX miceVoiding behaviorFemale C57BL6/J miceC57BL6/J micePost-menopausal womenEffects of ovariectomyBehavior changeMicturition changesTransurethral instillationLPS instillationTract infectionsSham surgeryLPS exposureJ miceCOX-2OVX surgeryDay 1Day 3Consecutive daysSurgeryMiceSpot assayGene expression changesLipopolysaccharide stimulates BK channel activity in bladder umbrella cells
Lu M, Li JR, Alvarez-Lugo L, Li Y, Yu S, Li X, Shi B, Chai TC. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. American Journal Of Physiology - Cell Physiology 2018, 314: c643-c653. PMID: 29466671, DOI: 10.1152/ajpcell.00339.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic AMP-Dependent Protein KinasesFemaleLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsLipopolysaccharide ReceptorsLipopolysaccharidesLymphocyte Antigen 96Membrane PotentialsMice, Inbred C57BLPatch-Clamp TechniquesPotassiumSignal TransductionToll-Like Receptor 4Urinary BladderUrinary Tract InfectionsUrotheliumConceptsBK channel activityBK channelsChannel activityUmbrella cellsUrinary tract infectionFemale C57BL6 micePotassium channel inhibitorsUropathogenic Escherichia coliProtein kinase ATract infectionsLPS effectsC57BL6 miceBladder urotheliumLamina propriaMin of exposureChannel inhibitorsBacterial infectionsLPS receptorUrothelial tissuesUrothelial cellsUrotheliumLipopolysaccharidePotassium channelsRT-PCRPKA blocker