2001
Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD
Wei Y, Lu M, Wang W. Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD. American Journal Of Physiology - Cell Physiology 2001, 280: c920-c928. PMID: 11245609, DOI: 10.1152/ajpcell.2001.280.4.c920.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzylaminesBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesDose-Response Relationship, DrugEnzyme InhibitorsFemaleIon Channel GatingIonomycinIonophoresKidney Tubules, CollectingMaleMembrane PotentialsNaphthalenesNG-Nitroarginine Methyl EsterNitratesPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseSpecific Pathogen-Free OrganismsStrophanthidinSulfonamidesSuperoxidesConceptsNitro-L-arginine methyl esterIntracellular Ca2Inhibitory effectNM Ca2Nitric oxideChannel activityEffects of strophanthidinKN-93KN-62Protein kinase CCalphostin CEffect of inhibitionCalmodulin-dependent kinase IICell-attached patchesExtracellular Ca2Rat CCDKinase CMicroM ionomycinRat kidneyMechanism of Ca2High concentrationsStrophanthidinCa2Methyl esterKinase II
1998
Neuronal nitric oxide synthase is expressed in principal cell of collecting duct
Wang X, Lu M, Gao Y, Papapetropoulos A, Sessa W, Wang W. Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. American Journal Of Physiology 1998, 275: f395-f399. PMID: 9729512, DOI: 10.1152/ajprenal.1998.275.3.f395.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseEndothelial nitric oxide synthasePresence of nNOSNitric oxide synthaseOxide synthasePrincipal cellsImmunocytochemical studyPresence of mRNART-PCR techniqueRat neuronal nitric oxide synthaseNegative immunostainingNNOS antibodyRat CCDRat kidneyImmunocytochemical methodsRT-PCRImmunoreactivityRatsDietCellsLight microscopyDuctSynthaseGene-specific primersKidneyReaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1997
Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct
Lu M, Giebisch G, Wang W. Nitric Oxide Links the Apical Na+ Transport to the Basolateral K+ Conductance in the Rat Cortical Collecting Duct. The Journal Of General Physiology 1997, 110: 717-726. PMID: 9382898, PMCID: PMC2229403, DOI: 10.1085/jgp.110.6.717.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsAnti-Arrhythmia AgentsBiological TransportCalciumCyclic GMPDiureticsEnzyme InhibitorsKidney Tubules, CollectingNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillaminePotassiumPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumSodium ChannelsSpecific Pathogen-Free OrganismsConceptsEffects of amilorideIntracellular Ca2Cell-attached patchesControl valuesChannel activityRat cortical collecting ductsNO-cGMP dependent pathwayNitric oxide synthaseFree bath solutionCortical collecting ductsNa/H exchangerPatch-clamp techniqueAddition of SNAPMicroM benzamilOxide synthaseChannel blockersFura-2MicroM amilorideExtracellular Ca2Rat CCDRemoval of Ca2Clamp techniqueNeuronal formCollecting ductsRat kidneyNitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD
Lu M, Giebisch G, Wang W. Nitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD. American Journal Of Physiology 1997, 272: f498-f504. PMID: 9140051, DOI: 10.1152/ajprenal.1997.272.4.f498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic GMPElectric ConductivityKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsModels, BiologicalNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillamineRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodium ChannelsTime FactorsConceptsL-NAMESodium nitroprussideNitric oxideNitro-L-arginine methyl esterL-NAME-induced decreaseAction of NOL-NAME resultsS-nitroso-N-acetyl penicillamineEffect of NOEffect of SNAPWhole-cell recordingsMicroM sodium nitroprussidePatch-clamp techniqueExogenous NO donorD-NAMENO synthaseMicroM amilorideRat CCDCell recordingsNO donorControl valuesStimulatory effectIndirect actionDependent pathwayHyperpolarization