2010
Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct
Lu M, Dong K, Egan ME, Giebisch GH, Boulpaep EL, Hebert SC. Mouse cystic fibrosis transmembrane conductance regulator forms cAMP-PKA–regulated apical chloride channels in cortical collecting duct. Proceedings Of The National Academy Of Sciences Of The United States Of America 2010, 107: 6082-6087. PMID: 20231442, PMCID: PMC2851921, DOI: 10.1073/pnas.0902661107.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoatesChloride ChannelsCyclic AMPCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorFemaleIn Vitro TechniquesKidney CortexKidney Tubules, CollectingKineticsMiceMice, Inbred C57BLMice, Inbred CFTRMice, KnockoutMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingRecombinant ProteinsThiazolidinesXenopus laevisConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCl- channel activityConductance regulatorCl- channelsApical membrane proteinsExpression of CFTRChannel activityCFTR Cl- channelApical chloride channelApical cell membraneDeltaF508 CFTR mutationMembrane proteinsCatalytic subunitXenopus laevis oocytesForm proteinPrincipal cellsCFTR channelsROMK null miceApical patchesApical membraneSingle-channel conductanceChloride channelsCell membrane
1998
Neuronal nitric oxide synthase is expressed in principal cell of collecting duct
Wang X, Lu M, Gao Y, Papapetropoulos A, Sessa W, Wang W. Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. American Journal Of Physiology 1998, 275: f395-f399. PMID: 9729512, DOI: 10.1152/ajprenal.1998.275.3.f395.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseEndothelial nitric oxide synthasePresence of nNOSNitric oxide synthaseOxide synthasePrincipal cellsImmunocytochemical studyPresence of mRNART-PCR techniqueRat neuronal nitric oxide synthaseNegative immunostainingNNOS antibodyRat CCDRat kidneyImmunocytochemical methodsRT-PCRImmunoreactivityRatsDietCellsLight microscopyDuctSynthaseGene-specific primersKidney