1998
Neuronal nitric oxide synthase is expressed in principal cell of collecting duct
Wang X, Lu M, Gao Y, Papapetropoulos A, Sessa W, Wang W. Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. American Journal Of Physiology 1998, 275: f395-f399. PMID: 9729512, DOI: 10.1152/ajprenal.1998.275.3.f395.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseEndothelial nitric oxide synthasePresence of nNOSNitric oxide synthaseOxide synthasePrincipal cellsImmunocytochemical studyPresence of mRNART-PCR techniqueRat neuronal nitric oxide synthaseNegative immunostainingNNOS antibodyRat CCDRat kidneyImmunocytochemical methodsRT-PCRImmunoreactivityRatsDietCellsLight microscopyDuctSynthaseGene-specific primersKidneyThe A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels
Ali S, Chen X, Lu M, Xu J, Lerea K, Hebert S, Wang W. The A kinase anchoring protein is required for mediating the effect of protein kinase A on ROMK1 channels. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10274-10278. PMID: 9707637, PMCID: PMC21498, DOI: 10.1073/pnas.95.17.10274.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCarrier ProteinsColforsinCyclic AMPCyclic AMP-Dependent Protein Kinase Type IICyclic AMP-Dependent Protein KinasesFemaleGTP-Binding ProteinsIn Vitro TechniquesKidneyOocytesPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Inwardly RectifyingRecombinant ProteinsXenopus laevisConceptsEffect of forskolinMicroM forskolinProtein kinase APatch-clamp techniqueKinase ACAMP-dependent pathwayKidney cortexNeuronal tissueForskolinLines of evidenceROMK channelsEffect of cAMPKidneyCAMP mimicsXenopus oocytesPresent studyType II protein kinase ASecretory channelsOocytesROMK1 channelsMicroMRIICAMPAddition of ATPMinReaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1995
Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney.
Wang W, Lu M. Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1995, 106: 727-743. PMID: 8576704, PMCID: PMC2229275, DOI: 10.1085/jgp.106.4.727.Peer-Reviewed Original ResearchConceptsThick ascending limbEffect of AAMicroM arachidonic acidArachidonic acidInhibitory effectAscending limbChannel activityAction of AARat kidneyMedullary thick ascending limbCytochrome P450 metabolic pathwayCytochrome P450 metabolitesPatch-clamp techniqueCell-attached patchesMicroM indomethacinP450 metabolitesMicroM linoleic acidStimulatory effectMetabolic pathwaysMain metaboliteKidneySpecific inhibitorLinoleic acidPredominant typeLimb