2001
Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD
Wei Y, Lu M, Wang W. Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD. American Journal Of Physiology - Cell Physiology 2001, 280: c920-c928. PMID: 11245609, DOI: 10.1152/ajpcell.2001.280.4.c920.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzylaminesBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesDose-Response Relationship, DrugEnzyme InhibitorsFemaleIon Channel GatingIonomycinIonophoresKidney Tubules, CollectingMaleMembrane PotentialsNaphthalenesNG-Nitroarginine Methyl EsterNitratesPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseSpecific Pathogen-Free OrganismsStrophanthidinSulfonamidesSuperoxidesConceptsNitro-L-arginine methyl esterIntracellular Ca2Inhibitory effectNM Ca2Nitric oxideChannel activityEffects of strophanthidinKN-93KN-62Protein kinase CCalphostin CEffect of inhibitionCalmodulin-dependent kinase IICell-attached patchesExtracellular Ca2Rat CCDKinase CMicroM ionomycinRat kidneyMechanism of Ca2High concentrationsStrophanthidinCa2Methyl esterKinase II
1998
Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1997
Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL
Wang W, Lu M, Balazy M, Hebert S. Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL. American Journal Of Physiology 1997, 273: f421-f429. PMID: 9321915, DOI: 10.1152/ajprenal.1997.273.3.f421.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumEstrenesFluorescent DyesFura-2GadoliniumHydroxyeicosatetraenoic AcidsKidney CortexKidney MedullaKineticsLoop of HenleMembrane PotentialsPatch-Clamp TechniquesPhosphodiesterase InhibitorsPhospholipases APhospholipases A2Potassium ChannelsPyrrolidinonesRatsRats, Sprague-DawleyTime FactorsConceptsThick ascending limbMicroM Gd3Phospholipase A2Extracellular Ca2Rat thick ascending limbAddition of thapsigarginEffects of stimulationInflux of Ca2Channel activityPatch-clamp techniquePhospholipase CCell-attached patchesMicroM gadoliniumFura-2Ascending limbFluorescent sodium indicatorIntracellular Ca2Rat kidneyInhibitory effectAcetoxymethyl esterInhibitionStimulationCa2Signaling mechanismSodium indicator
1996
Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney.
Lu M, Zhu Y, Balazy M, Reddy K, Falck J, Wang W. Effect of angiotensin II on the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1996, 108: 537-547. PMID: 8972391, PMCID: PMC2229337, DOI: 10.1085/jgp.108.6.537.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin IIAnimalsCell MembraneCytochrome P-450 Enzyme InhibitorsDose-Response Relationship, DrugEnzyme InhibitorsFatty Acids, UnsaturatedHydroxyeicosatetraenoic AcidsIn Vitro TechniquesLoop of HenleNaphthalenesNG-Nitroarginine Methyl EsterPatch-Clamp TechniquesPenicillaminePotassium Channel BlockersPotassium ChannelsRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodiumConceptsEffect of AIIThick ascending limbAngiotensin IIControl valuesInhibitory effectChannel activityAscending limbRat kidneyL-arginine methyl esterArachidonic acidStimulatory effectExogenous nitric oxide donorNM angiotensin IIMicroM NG-nitroNitric oxide synthaseMajor AA metaboliteNitric oxide donorMicroM calphostin CPatch-clamp techniqueP450-dependent pathwayL-NAMENG-nitroCell-attached patchesOxide synthaseP450 metabolitesCytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL
Wang W, Lu M, Hebert S. Cytochrome P-450 metabolites mediate extracellular Ca(2+)-induced inhibition of apical K+ channels in the TAL. American Journal Of Physiology 1996, 271: c103-c111. PMID: 8760035, DOI: 10.1152/ajpcell.1996.271.1.c103.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCalciumCell MembraneCytochrome P-450 Enzyme SystemExtracellular SpaceFatty Acids, UnsaturatedIn Vitro TechniquesLoop of HenleNeomycinPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleyTetradecanoylphorbol AcetateConceptsThick ascending limbCell-attached patchesChannel activityProtein kinase CCytochrome PArachidonic acidControl valuesInhibitory effectPatch-clamp techniqueEffects of neomycinCalphostin CMicroM neomycinExtracellular Ca2Ascending limbAddition of phorbolRat kidneyChannel open probabilityTAL tubulesNM calphostin CInhibition of PKCNitric oxide regulates the low-conductance K+ channel in basolateral membrane of cortical collecting duct
Lu M, Wang W. Nitric oxide regulates the low-conductance K+ channel in basolateral membrane of cortical collecting duct. American Journal Of Physiology 1996, 270: c1336-c1342. PMID: 8967433, DOI: 10.1152/ajpcell.1996.270.5.c1336.Peer-Reviewed Original ResearchConceptsNitric oxide synthaseCGMP-dependent pathwayL-NAMEL-NIOChannel activityL-arginine methyl esterExogenous nitric oxide donorOutward slope conductanceSlope conductanceNitric oxide donorPatch-clamp techniqueCell-attached patchesInward slope conductanceOxide synthaseBasolateral membraneSodium nitroprussideOxide donorRat kidneyNitric oxideIntermediate conductanceInhibitory effectEffect of NOTetraethylammonium chlorideMicroM NCyclic monophosphate
1995
Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney.
Wang W, Lu M. Effect of arachidonic acid on activity of the apical K+ channel in the thick ascending limb of the rat kidney. The Journal Of General Physiology 1995, 106: 727-743. PMID: 8576704, PMCID: PMC2229275, DOI: 10.1085/jgp.106.4.727.Peer-Reviewed Original ResearchConceptsThick ascending limbEffect of AAMicroM arachidonic acidArachidonic acidInhibitory effectAscending limbChannel activityAction of AARat kidneyMedullary thick ascending limbCytochrome P450 metabolic pathwayCytochrome P450 metabolitesPatch-clamp techniqueCell-attached patchesMicroM indomethacinP450 metabolitesMicroM linoleic acidStimulatory effectMetabolic pathwaysMain metaboliteKidneySpecific inhibitorLinoleic acidPredominant typeLimb