2003
Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium
Rauz S, Walker EA, Hughes SV, Coca-Prados M, Hewison M, Murray PI, Stewart PM. Serum- and Glucocorticoid-Regulated Kinase Isoform-1 and Epithelial Sodium Channel Subunits in Human Ocular Ciliary Epithelium. Investigative Ophthalmology & Visual Science 2003, 44: 1643-1651. PMID: 12657604, DOI: 10.1167/iovs.02-0514.Peer-Reviewed Original ResearchMeSH KeywordsAldosteroneBlotting, NorthernCell LineCiliary BodyDexamethasoneDose-Response Relationship, DrugDrug CombinationsEpithelial Sodium ChannelsGene Expression RegulationGlucocorticoidsHormone AntagonistsHumansImmediate-Early ProteinsIn Situ HybridizationIsoenzymesMifepristoneMineralocorticoid Receptor AntagonistsNuclear ProteinsPigment Epithelium of EyeProtein Serine-Threonine KinasesReceptors, GlucocorticoidReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSodium ChannelsSpironolactoneConceptsExpression of SGK1Human ocular ciliary epitheliumODM-2 cellsOcular ciliary epitheliumSodium transportCiliary epitheliumENaC subunitsNPE cell linesEpithelial sodium channel (ENaC) subunitsAqueous humor productionSodium channel subunitsExpression of ENaCRadioligand-binding assaysDose-dependent inductionIsoform 1Epithelial sodium channelCorticosteroid regulationHuman ocularAldosteroneCiliary epithelial bilayerRU26752DexamethasoneRT-PCR analysisSodium channelsChannel subunits
1997
Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium
Ortego J, Coca‐Prados M. Molecular Characterization and Differential Gene Induction of the Neuroendocrine‐Specific Genes Neurotensin, Neurotensin Receptor, PC1, PC2, and 7B2 in the Human Ocular Ciliary Epithelium. Journal Of Neurochemistry 1997, 69: 1829-1839. PMID: 9349525, DOI: 10.1046/j.1471-4159.1997.69051829.x.Peer-Reviewed Original ResearchMeSH KeywordsAspartic Acid EndopeptidasesCell LineCiliary BodyColforsinDexamethasoneDNA PrimersEnzyme InductionEpithelial CellsFurinGene Expression RegulationHumansIsoproterenolModels, BiologicalNerve Growth FactorsNeurosecretory SystemsNeurotensinPolymerase Chain ReactionProprotein Convertase 2Proprotein ConvertasesReceptors, NeurotensinSubtilisinsTetradecanoylphorbol AcetateTimololTranscriptional ActivationUp-RegulationConceptsOcular ciliary epitheliumDifferential gene inductionImportant physiological functionsHuman ocular ciliary epitheliumHuman cell linesTransduction pathwaysGene inductionGene expressionCiliary epitheliumBeta2-adrenergic receptorMolecular characterizationPhysiological functionsCell extractsODM-2Human ciliary epitheliumNeuroepithelial cellsSame inducersPhorbol esterCell linesNeuroendocrine tissuesExpressionGrowth factorParacrine mechanismsPC2 expressionNerve growth factor