1996
Functional expression of the cDNA encoded by the human ATP1AL1 gene
Grishin AV, Bevensee MO, Modyanov NN, Rajendran V, Boron WF, Caplan MJ. Functional expression of the cDNA encoded by the human ATP1AL1 gene. American Journal Of Physiology 1996, 271: f539-f551. PMID: 8853415, DOI: 10.1152/ajprenal.1996.271.3.f539.Peer-Reviewed Original ResearchConceptsHuman ATP1AL1 geneAcute loweringNH4Cl pulseUptake activityHEK-293 cellsSpontaneous intracellularMM ouabainProtein expressionCOS cellsATP1AL1Polyclonal antibodiesPH-sensitive dyeOuabainBeta-subunit cDNAExtrusion activityCellsK-ATPaseTransfection studiesSame cellsFunctional expressionBeta complexExpressionDependent ATPaseEndplasmic reticulumProtein
1987
Localization of Na+,K+-ATPase alpha-subunit to the sinusoidal and lateral but not canalicular membranes of rat hepatocytes.
Sztul ES, Biemesderfer D, Caplan MJ, Kashgarian M, Boyer JL. Localization of Na+,K+-ATPase alpha-subunit to the sinusoidal and lateral but not canalicular membranes of rat hepatocytes. Journal Of Cell Biology 1987, 104: 1239-1248. PMID: 3032985, PMCID: PMC2114466, DOI: 10.1083/jcb.104.5.1239.Peer-Reviewed Original ResearchConceptsAlpha-subunit bandsRat liver plasma membrane fractionsPlasma membraneAlpha subunitCatalytic activityMembrane fractionEntire plasma membranePlasma membrane fractionATPase alpha subunitIon-transporting epitheliaMonoclonal antibodiesWestern blotLiver plasma membrane fractionsBasolateral domainBasolateral distributionPolyclonal antibodies