2001
CAK-independent Activation of CDK6 by a Viral Cyclin
Kaldis P, Ojala P, Tong L, Mäkelä T, Solomon M. CAK-independent Activation of CDK6 by a Viral Cyclin. Molecular Biology Of The Cell 2001, 12: 3987-3999. PMID: 11739795, PMCID: PMC60770, DOI: 10.1091/mbc.12.12.3987.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisCyclin-Dependent Kinase 6Cyclin-Dependent Kinase Inhibitor p16Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsEnzyme ActivationFluorescent Antibody TechniqueHerpesvirus 8, HumanHumansPhosphorylationProtein ConformationProtein Serine-Threonine KinasesTumor Cells, CulturedViral ProteinsConceptsKSHV-cyclinSarcoma-associated herpesvirusKaposi's sarcoma-associated herpesvirusCell cycle progression independentAbsence of phosphorylationCyclin-dependent kinasesD-type cyclinsCAK phosphorylationExpression of CDK6CDK6 activationMitogenic signalsSubstrate specificityCell-based assaysCDK inhibitorsViral cyclinConformational changesCell deathPhosphorylationCAKCDK inhibitionKinaseCyclinCDK6Ternary complexNormal cells
2000
Dephosphorylation of Human Cyclin-dependent Kinases by Protein Phosphatase Type 2Cα and β2 Isoforms*
Cheng A, Kaldis P, Solomon M. Dephosphorylation of Human Cyclin-dependent Kinases by Protein Phosphatase Type 2Cα and β2 Isoforms*. Journal Of Biological Chemistry 2000, 275: 34744-34749. PMID: 10934208, DOI: 10.1074/jbc.m006210200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsChromatography, Ion ExchangeCyclin-Dependent KinasesCyclinsHeLa CellsHumansIsoenzymesMiceMolecular Sequence DataPhosphoprotein PhosphatasesPhosphorylationProtein Phosphatase 2Protein Phosphatase 2CRatsSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSubstrate SpecificityConceptsHeLa cell extractsCyclin-dependent kinasesPP2C alphaType 2C protein phosphatasesHuman cyclin-dependent kinaseCell extractsBeta 2 isoformBinding of cyclinsDephosphorylation of cdk2Mono Q chromatographyBeta 2 proteinProtein phosphataseThreonine residuesSubstrate preferenceBeta 2Beta isoformsΒ2 isoformPhosphatase activityIsoformsDephosphorylationDEAE-SepharoseSuperdex 200KinasePhosphorylationCDK6Activating Phosphorylation of the Saccharomyces cerevisiae Cyclin-dependent Kinase, Cdc28p, Precedes Cyclin Binding
Ross K, Kaldis P, Solomon M. Activating Phosphorylation of the Saccharomyces cerevisiae Cyclin-dependent Kinase, Cdc28p, Precedes Cyclin Binding. Molecular Biology Of The Cell 2000, 11: 1597-1609. PMID: 10793138, PMCID: PMC14870, DOI: 10.1091/mbc.11.5.1597.Peer-Reviewed Original ResearchMeSH KeywordsAntibody SpecificityCDC28 Protein Kinase, S cerevisiaeCell CycleCyclin ACyclin BCyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsEnzyme ActivationEpitopesMutationPhosphorylationProtein Serine-Threonine KinasesRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsThreonineConceptsCyclin bindingCyclin-dependent kinasesEukaryotic cell cycle progressionConserved threonine residueAddition of cyclinCell cycle progressionHigher eukaryotesThreonine residuesCdc28pCDK activationProtein kinaseRegulatory mechanismsCycle progressionCell cycleCyclinKinasePhosphorylationCak1pActivation pathwayCDKBindingEukaryotesActivationMutantsThreonine
1999
Transforming growth factor β targeted inactivation of cyclin E:cyclin-dependent kinase 2 (Cdk2) complexes by inhibition of Cdk2 activating kinase activity
Nagahara H, Ezhevsky S, Vocero-Akbani A, Kaldis P, Solomon M, Dowdy S. Transforming growth factor β targeted inactivation of cyclin E:cyclin-dependent kinase 2 (Cdk2) complexes by inhibition of Cdk2 activating kinase activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 14961-14966. PMID: 10611320, PMCID: PMC24755, DOI: 10.1073/pnas.96.26.14961.Peer-Reviewed Original ResearchMeSH KeywordsCDC2-CDC28 KinasesCyclin ECyclin HCyclin-Dependent Kinase 2Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsDown-RegulationEnzyme ActivationG1 PhaseHumansModels, BiologicalPhosphorylationProtein Serine-Threonine KinasesThreonineTransforming Growth Factor betaTumor Cells, CulturedConceptsTGF-beta treatmentInhibition of CDK2Cyclin ECyclin DGrowth factor betaGrowth factor βHepG2 hepatocellular carcinoma cellsHepatocellular carcinoma cellsTGF-beta signalingHuman HepG2 hepatocellular carcinoma cellsFactor betaCarcinoma cellsCyclin-dependent kinase 2 complexFactor βKinase activityCDK7 activityCDK4/6 activityHepG2 cellsWhole cell lysatesTumor suppressor proteinCDK2TreatmentCdk2 complexesArrestCells
1998
Human and Yeast Cdk-activating Kinases (CAKs) Display Distinct Substrate Specificities
Kaldis P, Russo A, Chou H, Pavletich N, Solomon M. Human and Yeast Cdk-activating Kinases (CAKs) Display Distinct Substrate Specificities. Molecular Biology Of The Cell 1998, 9: 2545-2560. PMID: 9725911, PMCID: PMC25525, DOI: 10.1091/mbc.9.9.2545.Peer-Reviewed Original ResearchConceptsTranscription factor IIHC-terminal domainSubstrate specificityCDK/cyclin complexesCTD kinase activityRNA polymerase IIDistinct substrate specificitiesDifferent substrate specificitiesCyclin-dependent kinasesCell cycle progressionHuman CAKYeast CdkEnzyme-substrate recognitionPolymerase IILarge subunitTranscriptional componentsCak1pCDK activationCyclin complexesKey residuesKinase activitySingle polypeptideCycle progressionCDK inhibitorsCDK
1996
A Predictive Scale for Evaluating Cyclin-dependent Kinase Substrates A COMPARISON OF p34 cdc2 AND p33 cdk2 *
Holmes J, Solomon M. A Predictive Scale for Evaluating Cyclin-dependent Kinase Substrates A COMPARISON OF p34 cdc2 AND p33 cdk2 *. Journal Of Biological Chemistry 1996, 271: 25240-25246. PMID: 8810285, DOI: 10.1074/jbc.271.41.25240.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesCDC2 Protein KinaseCDC2-CDC28 KinasesCell CycleCell LineConsensus SequenceCyclin-Dependent Kinase 2Cyclin-Dependent KinasesCyclinsGlutathione TransferaseHumansMutagenesis, Site-DirectedOligopeptidesPolymerase Chain ReactionProtein Serine-Threonine KinasesRecombinant Fusion ProteinsSpodopteraSubstrate SpecificityTransfectionXenopusXenopus ProteinsConceptsSubstrate specificityDistinct substrate specificitiesPrimary sequence determinantsCyclin-dependent kinasesCDK substratesCyclin subunitProtein phosphorylationProtein kinaseCDK familyP34 cdc2Consensus sequenceSequence determinantsCell cyclePhosphorylation efficiencyKinasePeptide fusionsPhosphorylation potentialSignificant oversimplificationCdc2PhosphorylationSpecificitySubunitsCDK2SpeciesTrue specificityThe Cdk-Activating Kinase (CAK) from Budding Yeast
Kaldis P, Sutton A, Solomon M. The Cdk-Activating Kinase (CAK) from Budding Yeast. Cell 1996, 86: 553-564. PMID: 8752210, DOI: 10.1016/s0092-8674(00)80129-4.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCDC28 Protein Kinase, S cerevisiaeCDC2-CDC28 KinasesCell CycleCell Cycle ProteinsCyclin BCyclin-Dependent Kinase 2Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsFungal ProteinsGenes, FungalMolecular Sequence DataMolecular WeightPhosphorylationProtein Serine-Threonine KinasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino AcidStructure-Activity RelationshipConceptsCDK-Activating KinaseBasal transcription factorsTemperature-sensitive mutationProtein kinase activityCyclin-dependent kinasesCell cycle progressionGenetic interactionsMitotic cyclinsTranscription factorsS. cerevisiaeKinase activityCycle progressionCell extractsG2 delayKinaseAltered expressionE. coliIntriguing possibilityPhosphorylationCyclinFull activityCak1pClb2TFIIHCak1
1994
Inactivation of a Cdk2 inhibitor during interleukin 2-induced proliferation of human T lymphocytes.
Firpo E, Koff A, Solomon M, Roberts J. Inactivation of a Cdk2 inhibitor during interleukin 2-induced proliferation of human T lymphocytes. Molecular And Cellular Biology 1994, 14: 4889-4901. PMID: 7516474, PMCID: PMC358861, DOI: 10.1128/mcb.14.7.4889.Peer-Reviewed Original ResearchMeSH KeywordsAdultCDC2-CDC28 KinasesCell CycleCells, CulturedCyclin-Dependent Kinase 2Cyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent KinasesCyclinsDNAEnzyme ActivationFlow CytometryHumansInterleukin-2KineticsLymphocyte ActivationProtein Kinase InhibitorsProtein KinasesProtein Serine-Threonine KinasesReceptors, Antigen, T-CellReceptors, Interleukin-2Recombinant ProteinsRNASignal TransductionTime FactorsT-LymphocytesConceptsCyclin-dependent kinasesMitogenic signalsCyclin-cdk2 complexesCDK2 inhibitorsNegative growth signalsG1 cyclin-CDK complexesCell cycle commitmentCyclin-CDK complexesCyclin-Cdk inhibitorT cell antigen receptorCell proliferationAntigen receptor stimulationCell cycle proteinsInhibitors of CDK2Mitogenic growth factorsGrowth signalsSimilar proteinsBiochemical pathwaysCell cycleCDK inhibitorsCdk2 activationCycle proteinsRestriction pointS phaseCommon targetp27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest.
Polyak K, Kato J, Solomon M, Sherr C, Massague J, Roberts J, Koff A. p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest. Genes & Development 1994, 8: 9-22. PMID: 8288131, DOI: 10.1101/gad.8.1.9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCDC2-CDC28 KinasesCell CommunicationCell CycleCell LineCyclin-Dependent Kinase 2Cyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesCyclinsMinkProtein Kinase InhibitorsProtein KinasesProtein Serine-Threonine KinasesProteinsProtein-Tyrosine KinasesSignal TransductionTransforming Growth Factor betaConceptsCdk2 complexesCyclin ECyclin D2-Cdk4 complexesCyclin-Cdk inhibitorCell-cell contactGrowth inhibitory signalsCell cycle arrestCatalytic subunitG1 progressionG1 cyclinsCell cycleCdk2 activationCycle arrestCDK2 inhibitionContact inhibitionCyclin E levelsActive complexAffinity chromatographyEpithelial cellsCDK2Inhibitory signalsCellsP27ComplexesActivation
1993
CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15.
Solomon M, Harper J, Shuttleworth J. CAK, the p34cdc2 activating kinase, contains a protein identical or closely related to p40MO15. The EMBO Journal 1993, 12: 3133-3142. PMID: 8344252, PMCID: PMC413579, DOI: 10.1002/j.1460-2075.1993.tb05982.x.Peer-Reviewed Original ResearchPhosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro.
Connell-Crowley L, Solomon M, Wei N, Harper J. Phosphorylation independent activation of human cyclin-dependent kinase 2 by cyclin A in vitro. Molecular Biology Of The Cell 1993, 4: 79-92. PMID: 8443411, PMCID: PMC300902, DOI: 10.1091/mbc.4.1.79.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCDC2-CDC28 KinasesCyclin-Dependent Kinase 2Cyclin-Dependent KinasesCyclinsDNAEnzyme ActivationHumansIn Vitro TechniquesMolecular Sequence DataMutagenesis, Site-DirectedPhosphorylationProtein KinasesProtein Serine-Threonine KinasesSaccharomyces cerevisiaeXenopusXenopus ProteinsConceptsCDK2/cyclinCyclin-dependent kinase 2Cyclin AKinase 2Cyclin BPhosphorylation-independent activationSerine-threonine proteinCdc2/cyclin BCDK2/cyclin AHuman cyclin-dependent kinase 2G1/S transitionHistone H1 kinaseCyclin B complexCell cycle progressionSpecific activityCyclin bindingRecombinant CDK2Mammalian cellsH1 kinaseBacterial expressionT160 phosphorylationCycle progressionXenopus eggsCell extractsCyclin