Featured Publications
Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq
Saraiva L, Ibarra-Soria X, Khan M, Omura M, Scialdone A, Mombaerts P, Marioni J, Logan D. Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq. Scientific Reports 2015, 5: 18178. PMID: 26670777, PMCID: PMC4680959, DOI: 10.1038/srep18178.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCluster AnalysisComputational BiologyFemaleGene Expression ProfilingHigh-Throughput Nucleotide SequencingMaleMiceOlfactory MucosaOlfactory Receptor NeuronsReceptors, OdorantTranscriptomeConceptsMature olfactory sensory neuronsOlfactory sensory neuronsRNA-seqOR genesGene expressionSingle-cell RNA-seqIntact OR genesOlfactory receptorsCell typesSingle-nucleotide polymorphismsOR-expressing OSNsOR gene expressionIntact olfactory receptorsNeuron-one receptor ruleCrude tissue samplesMonoallelic expressionNeuronal cell typesMouse olfactory mucosaMultiple cell typesChemosensory tissuesGenesCellular heterogeneityOlfactory mucosaSensory neuronsNon-neuronalHigh-throughput spatial mapping of single-cell RNA-seq data to tissue of origin
Achim K, Pettit J, Saraiva L, Gavriouchkina D, Larsson T, Arendt D, Marioni J. High-throughput spatial mapping of single-cell RNA-seq data to tissue of origin. Nature Biotechnology 2015, 33: 503-509. PMID: 25867922, DOI: 10.1038/nbt.3209.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGene Expression Regulation, DevelopmentalHigh-Throughput Nucleotide SequencingOrgan SpecificityPolychaetaSingle-Cell AnalysisTranscriptome
2015
Molecular and neuronal homology between the olfactory systems of zebrafish and mouse
Saraiva L, Ahuja G, Ivandic I, Syed A, Marioni J, Korsching S, Logan D. Molecular and neuronal homology between the olfactory systems of zebrafish and mouse. Scientific Reports 2015, 5: 11487. PMID: 26108469, PMCID: PMC4480006, DOI: 10.1038/srep11487.Peer-Reviewed Original ResearchConceptsDegree of molecular conservationYears of evolutionary divergenceVomeronasal organOlfactory mucosaOlfactory organChemosensory receptor genesOlfactory systemTranscriptome of miceDual olfactory systemEvolutionary divergenceMolecular conservationNeuronal homologRepertoire sizeRNA abundanceMolecular relationshipsCell-specific markersClasses of neuronsMolecular basisChemosensory receptorsMarker genesOrgans of rodentsAbsolute abundanceZebrafishReceptor geneVertebrates
2014
The Olfactory Transcriptomes of Mice
Ibarra-Soria X, Levitin M, Saraiva L, Logan D. The Olfactory Transcriptomes of Mice. PLOS Genetics 2014, 10: e1004593. PMID: 25187969, PMCID: PMC4154679, DOI: 10.1371/journal.pgen.1004593.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFemaleGene ExpressionGenomeHigh-Throughput Nucleotide SequencingMaleMiceMice, Inbred C57BLOlfactory MucosaOlfactory PerceptionPseudogenesReceptors, OdorantSmellTranscriptomeVomeronasal OrganConceptsVomeronasal receptorsVR genesProtein-coding genesProtein coding sequencesDeep RNA sequencingDistribution of abundanceEvidence of expressionCell-specific expressionReference genomeGene annotationMultiple malesMulti-exonQuantitative catalogOlfactory tissueMouse genomeCoding sequenceOR genesOlfactory-mediated behaviorsGenomic techniquesExpression microarraysRNA sequencingGene expressionGenesQuantitative RT-PCRSexual dimorphism