2007
Slack and Slick KNa Channels Regulate the Accuracy of Timing of Auditory Neurons
Yang B, Desai R, Kaczmarek LK. Slack and Slick KNa Channels Regulate the Accuracy of Timing of Auditory Neurons. Journal Of Neuroscience 2007, 27: 2617-2627. PMID: 17344399, PMCID: PMC6672517, DOI: 10.1523/jneurosci.5308-06.2007.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsAnimals, NewbornAuditory PathwaysBithionolBrain StemComputer SimulationElectric ConductivityElectric StimulationElectrophysiologyIn Vitro TechniquesMiceModels, NeurologicalNerve Tissue ProteinsNeuronsNeurons, AfferentPotassium ChannelsPotassium Channels, Sodium-ActivatedReaction TimeSodium
2004
Proapoptotic N-truncated BCL-xL protein activates endogenous mitochondrial channels in living synaptic terminals
Jonas EA, Hickman JA, Chachar M, Polster BM, Brandt TA, Fannjiang Y, Ivanovska I, Basañez G, Kinnally KW, Zimmerberg J, Hardwick JM, Kaczmarek LK. Proapoptotic N-truncated BCL-xL protein activates endogenous mitochondrial channels in living synaptic terminals. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 13590-13595. PMID: 15342906, PMCID: PMC518799, DOI: 10.1073/pnas.0401372101.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-X ProteinDecapodiformesElectric ConductivityEndopeptidasesHypoxiaIon ChannelsLiposomesMitochondriaNADPatch-Clamp TechniquesPorinsPresynaptic TerminalsProtein Processing, Post-TranslationalProto-Oncogene Proteins c-bcl-2Sequence DeletionVoltage-Dependent Anion ChannelsConceptsBcl-xLMitochondrial channelsDeath pathwaysMitochondrial membraneBcl-xL.Proapoptotic Bcl-2 family proteinsVoltage-dependent anion channelBcl-2 family proteinsOuter mitochondrial membraneCell death pathwaysHydrophobic C-terminusBcl-xL proteinAntiapoptotic Bcl-xLNeuronal death pathwaysDeath stimuliBH3 domainFamily proteinsSquid presynaptic terminalsMammalian cellsC-terminusAnion channelMitochondriaChannel activityOpposite effectHealthy neurons
2003
Slick (Slo2.1), a Rapidly-Gating Sodium-Activated Potassium Channel Inhibited by ATP
Bhattacharjee A, Joiner WJ, Wu M, Yang Y, Sigworth FJ, Kaczmarek LK. Slick (Slo2.1), a Rapidly-Gating Sodium-Activated Potassium Channel Inhibited by ATP. Journal Of Neuroscience 2003, 23: 11681-11691. PMID: 14684870, PMCID: PMC6740956, DOI: 10.1523/jneurosci.23-37-11681.2003.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAmino Acid SequenceAnimalsCells, CulturedChloridesCHO CellsCloning, MolecularCricetinaeElectric ConductivityHumansIon Channel GatingKineticsMolecular Sequence DataPotassium ChannelsPotassium Channels, Sodium-ActivatedRatsSequence AlignmentSodiumTissue DistributionXenopusFunctional Specialization of Male and Female Vocal Motoneurons
Yamaguchi A, Kaczmarek LK, Kelley DB. Functional Specialization of Male and Female Vocal Motoneurons. Journal Of Neuroscience 2003, 23: 11568-11576. PMID: 14684859, PMCID: PMC6740944, DOI: 10.1523/jneurosci.23-37-11568.2003.Peer-Reviewed Original ResearchModulation of the Kv3.1b Potassium Channel Isoform Adjusts the Fidelity of the Firing Pattern of Auditory Neurons
Macica CM, von Hehn CA, Wang LY, Ho CS, Yokoyama S, Joho RH, Kaczmarek LK. Modulation of the Kv3.1b Potassium Channel Isoform Adjusts the Fidelity of the Firing Pattern of Auditory Neurons. Journal Of Neuroscience 2003, 23: 1133-1141. PMID: 12598601, PMCID: PMC6742259, DOI: 10.1523/jneurosci.23-04-01133.2003.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsBrain StemCells, CulturedCHO CellsCricetinaeElectric ConductivityEvoked Potentials, AuditoryKineticsMiceMice, KnockoutNeuronsNeuropeptidesPatch-Clamp TechniquesPhosphorylationPotassium ChannelsPotassium Channels, Voltage-GatedProtein IsoformsProtein Kinase CSerineShaw Potassium ChannelsTetradecanoylphorbol AcetateConceptsTrapezoid bodyMedial nucleusAuditory neuronsHigh-frequency stimulationWild-type neuronsKv3.1 potassium channelHigh-threshold componentPotassium channel isoformsGreat temporal precisionPartial decreaseProtein kinase C activationAction potentialsLocation of soundsMice resultsFiring patternsNeuronsSensory stimulationPotassium channelsChannel isoformsKinase C activationKv3.1Kv3.1 geneStimulationHigh frequencyProtein kinase C
2002
HSV-1 Helper Virus 5dl1.2 Suppresses Sodium Currents in Amplicon-Transduced Neurons
White BH, Cummins TR, Wolf DH, Waxman SG, Russell DS, Kaczmarek LK. HSV-1 Helper Virus 5dl1.2 Suppresses Sodium Currents in Amplicon-Transduced Neurons. Journal Of Neurophysiology 2002, 87: 2149-2157. PMID: 11929932, DOI: 10.1152/jn.00498.2001.Peer-Reviewed Original ResearchConceptsSodium currentAnti-HSV antibodiesAverage spike frequencyWild-type HSV-1Helper virusViral-based strategiesDays of transductionCultured neuronsHSV-1Spike frequencyGene deliveryNeuronsMammalian neuronsAmplicon systemSimilar suppressionHSV-1 genesVirusTherapeutic purposesViral proteinsAmplicon preparationsCellsCoinfectionSuppressionPreparation resultsTiters
1999
Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission
Jonas E, Buchanan J, Kaczmarek L. Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science 1999, 286: 1347-1350. PMID: 10558987, DOI: 10.1126/science.286.5443.1347.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium ChannelsDecapodiformesElectric ConductivityElectric StimulationIntracellular MembranesIon ChannelsIon TransportMicroscopy, ElectronMitochondriaPatch-Clamp TechniquesPorinsPresynaptic TerminalsSynaptic TransmissionTime FactorsVoltage-Dependent Anion ChannelsConceptsChannel activityIon channel activityMitochondrial membraneOnly organellesIntracellular organellesIntact cellsIon channelsMitochondriaOrganellesLarge conductanceTens of secondsPresynaptic terminalsIon transportSynaptic transmissionSynaptic stimulationConductanceElectron microscopyPatch-clamp techniqueMembraneActivityCellsActivationSquidStimulation
1998
Formation of intermediate-conductance calcium-activated potassium channels by interaction of Slack and Slo subunits
Joiner W, Tang M, Wang L, Dworetzky S, Boissard C, Gan L, Gribkoff V, Kaczmarek L. Formation of intermediate-conductance calcium-activated potassium channels by interaction of Slack and Slo subunits. Nature Neuroscience 1998, 1: 462-469. PMID: 10196543, DOI: 10.1038/2176.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCaenorhabditis elegansCaenorhabditis elegans ProteinsElectric ConductivityIntermediate-Conductance Calcium-Activated Potassium ChannelsIsomerismLarge-Conductance Calcium-Activated Potassium ChannelsMolecular Sequence DataNerve Tissue ProteinsPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium Channels, Sodium-ActivatedConceptsCalcium-activated potassium channelsIntracellular calciumNervous systemIntermediate-conductance calcium-activated potassium channelsPotassium channelsLarge-conductance calcium-activated potassium channelsControl of excitabilitySlo subunitIntermediate conductance channelPotassium channel genesPharmacological propertiesIntermediate conductanceCytoplasmic calciumChannel subunitsSlo channelsSlack channelsChannel genesSingle-channel conductanceUnitary conductanceCalciumExcitabilitySLOSecretion
1997
The Secretion of Classical and Peptide Cotransmitters from a Single Presynaptic Neuron Involves a Synaptobrevin-Like Molecule
Whim M, Niemann H, Kaczmarek L. The Secretion of Classical and Peptide Cotransmitters from a Single Presynaptic Neuron Involves a Synaptobrevin-Like Molecule. Journal Of Neuroscience 1997, 17: 2338-2347. PMID: 9065494, PMCID: PMC6573516, DOI: 10.1523/jneurosci.17-07-02338.1997.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsAplysiaCalciumCells, CulturedCoculture TechniquesElectric ConductivityGanglia, InvertebrateKineticsMagnesiumMembrane PotentialsMembrane ProteinsNerve Tissue ProteinsNeuronsNeurons, AfferentNeuropeptidesPatch-Clamp TechniquesPresynaptic TerminalsRecombinant ProteinsR-SNARE ProteinsSynapsesTetanus ToxinConceptsClassical transmittersSingle presynaptic neuronRelease of neuropeptidesSingle action potentialPresynaptic release sitesSecretion of peptidesNeuron B2Peptidergic synapsesSynaptic typesSensory neuronsPresynaptic neuronsTetanus toxinPeptide cotransmittersAction potentialsPresynaptic injectionSecretionNeuronsMolecular mechanismsSynapseTypes of transmittersB2CotransmitterNeuropeptidesPeptidesRelease
1996
Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons
Nick T, Kaczmarek L, Carew T. Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons. Journal Of Neuroscience 1996, 16: 7583-7598. PMID: 8922415, PMCID: PMC6579091, DOI: 10.1523/jneurosci.16-23-07583.1996.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumCell SeparationCellular SenescenceElectric ConductivityElectrophysiologyGanglia, InvertebrateIonsNeuronsPatch-Clamp TechniquesPotassiumConceptsBag cell neuronsRepetitive firingCell neuronsEgg-laying behaviorJuvenile neuronsTetraethyl ammonium chlorideDevelopmental regulationSpecific ionic currentsAplysia bag cell neuronsAfterdischargesElectrical stimulationVariety of stimuliDevelopmental controlNeuronal systemsProlonged depolarizationBehavioral effectsNeuronsIonic currentsDevelopmental mechanismsSelective regulationAdultsAplysia californicaFiringRegulationDepolarization
1995
Modulation of the inactivation of voltage-dependent potassium channels by cAMP
Chung S, Kaczmarek L. Modulation of the inactivation of voltage-dependent potassium channels by cAMP. Journal Of Neuroscience 1995, 15: 3927-3935. PMID: 7751955, PMCID: PMC6578223, DOI: 10.1523/jneurosci.15-05-03927.1995.Peer-Reviewed Original Research
1992
Expression of the H-ras oncogene induces potassium conductance and neuron-specific potassium channel mRNAs in the AtT20 cell line
Hemmick L, Perney T, Flamm R, Kaczmarek L, Birnberg N. Expression of the H-ras oncogene induces potassium conductance and neuron-specific potassium channel mRNAs in the AtT20 cell line. Journal Of Neuroscience 1992, 12: 2007-2014. PMID: 1607925, PMCID: PMC6575920, DOI: 10.1523/jneurosci.12-06-02007.1992.Peer-Reviewed Original ResearchMeSH KeywordsElectric ConductivityElectrophysiologyGene ExpressionGenes, rasNeuronsPotassiumPotassium ChannelsRNA, MessengerTumor Cells, CulturedConceptsRas-transfected cellsAtT20 cell lineAtT20 cellsEJ-ras oncogeneCell linesPotassium channelsVoltage-dependent potassium channelsRas proteinsPituitary-derived cellsMRNA speciesPlasma membraneSpliced productsPotassium channel mRNASame geneChannel mRNADifferential expressionPotassium currentExcitable cellsVoltage-dependent potassium currentsTwo- to threefold increaseMRNAOncogeneSpeciesExpressionPhenotype
1990
Estrogen induction of a small, putative K+ channel mRNA in rat uterus
Pragnell M, Snay K, Trimmer J, MacLusky N, Naftolin F, Kaczmarek L, Boyle M. Estrogen induction of a small, putative K+ channel mRNA in rat uterus. Neuron 1990, 4: 807-812. PMID: 2344412, DOI: 10.1016/0896-6273(90)90207-v.Peer-Reviewed Original ResearchConceptsMRNA speciesAmino acid proteinProkaryotic ion channelsDramatic long-term changesMolecular cloningAcid proteinIon channel expressionMammalian sourcesIon channelsXenopus oocytesVoltage-dependent channelsSpeciesStructural motifsCritical roleChannel expressionMRNAChannel mRNAEstrogen inductionLong-term changesInductionCloningProteinMotifRegulationOocytesTransfection of activated ras into an excitable cell line (AtT-20) alters tetrodotoxin sensitivity of voltage-dependent sodium current
Flamm R, Birnberg N, Kaczmarek L. Transfection of activated ras into an excitable cell line (AtT-20) alters tetrodotoxin sensitivity of voltage-dependent sodium current. Pflügers Archiv - European Journal Of Physiology 1990, 416: 120-125. PMID: 2191273, DOI: 10.1007/bf00370232.Peer-Reviewed Original ResearchConceptsTTX-sensitive currentsResistant currentSodium currentTTX-resistant sodium currentsVoltage-dependent sodium currentsSodium channel blocker tetrodotoxinTTX-resistant currentChannel blocker tetrodotoxinAnterior pituitary tumorsVoltage-dependent sodiumCell linesC-Ha-ras geneTTX-sensitiveBlocker tetrodotoxinPituitary tumorsExcitable cell lineC-Ha-ras oncogeneTetrodotoxin sensitivityTetrodotoxinAtT-20 cellsControl cellsCellsRate of inactivationTransfectionTumors
1987
Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons
Strong J, Fox A, Tsien R, Kaczmarek L. Stimulation of protein kinase C recruits covert calcium channels in Aplysia bag cell neurons. Nature 1987, 325: 714-717. PMID: 2434853, DOI: 10.1038/325714a0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumDiglyceridesElectric ConductivityEnzyme ActivationIon ChannelsNeuronsNeurosecretory SystemsProtein Kinase CTetradecanoylphorbol AcetateConceptsProtein kinase CBag cell neuronsKinase CProtein kinaseCyclic AMP-dependent protein kinaseAMP-dependent protein kinaseAplysia bag cell neuronsCell neuronsCalcium channelsSingle-channel levelExcitable cellsKinaseUntreated cellsUnitary conductanceCalcium currentPeptidergic bag cell neuronsVoltage-activated calcium channelsCardiac muscleDifferent unitary conductancesCellsDifferent mechanismsVoltage-activated calcium currentsChannel levelSecond classNeurons
1986
Phorbol Esters, Protein Phosphorylation and the Regulation of Neuronal Ion Channels
Kaczmarek L. Phorbol Esters, Protein Phosphorylation and the Regulation of Neuronal Ion Channels. Journal Of Experimental Biology 1986, 124: 375-392. PMID: 2428907, DOI: 10.1242/jeb.124.1.375.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumDiglyceridesElectric ConductivityEnzyme ActivationIon ChannelsNeuronsPhorbol EstersPhosphorylationProtein Kinase CTetradecanoylphorbol AcetateConceptsProtein kinase CKinase CProtein kinase C resultsPhorbol esterNeuronal ion channelsProtein phosphorylationPlasma membraneLipid environmentIntact cellsIon channelsSuch activatorsEndogenous diacylglycerolVertebratesDiacylglycerolSynthetic diacylglycerolPhosphatidyl serineActivatorEndogenous electrical propertiesInactive speciesSynaptic transmissionInvertebratesCalcium channelsKinasePhosphorylationNervous systemChapter 7 The role of protein kinases in the control of prolonged changes in neuronal excitability
Kaczmarek L, Strong J, Kauer J. Chapter 7 The role of protein kinases in the control of prolonged changes in neuronal excitability. Progress In Brain Research 1986, 69: 77-90. PMID: 3328878, DOI: 10.1016/s0079-6123(08)61050-x.Chapters
1984
A voltage-clamp analysis of currents underlying cyclic AMP-induced membrane modulation in isolated peptidergic neurons of Aplysia
Kaczmarek L, Strumwasser F. A voltage-clamp analysis of currents underlying cyclic AMP-induced membrane modulation in isolated peptidergic neurons of Aplysia. Journal Of Neurophysiology 1984, 52: 340-349. PMID: 6090605, DOI: 10.1152/jn.1984.52.2.340.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsCyclic AMP analoguePotassium currentOutward currentsNormal ionic mediumOnset of afterdischargePhosphodiesterase-resistant cAMP analoguePeak inward currentsAMP analogueBag cell clustersDelayed potassium currentMinority of cellsSpontaneous dischargeCalcium currentPeptidergic neuronsVoltage-clamp analysisCell culturesTwo-electrode voltage clampInward currentsSodium currentNegative slope resistanceA-currentMarked reductionNeurons