2015
Membrane Ion Channels and Ion Currents
Levitan I, Kaczmarek L. Membrane Ion Channels and Ion Currents. 2015, 63-84. DOI: 10.1093/med/9780199773893.003.0004.ChaptersSingle ion channelsIon currentMovement of ionsIon channelsParticular ionPlasma membraneSpecialized membrane proteinsMembrane ion channelsIonsAction potential firingNeuronal plasma membranePatch-clamp techniqueMembrane proteinsNeurons resultsCurrentClamp techniqueAction potentialsDetailed characterizationElectrical activityMembrane currentsMembrane voltageChannelsMacroscopic membrane currentsEssential propertiesComplex patternsDiversity in the Structure and Function of Ion Channels
Levitan I, Kaczmarek L. Diversity in the Structure and Function of Ion Channels. 2015, 127-150. DOI: 10.1093/med/9780199773893.003.0007.ChaptersIon channelsPotassium channelsFormation of heterotetramersAlternative splicingIndividual genesMultiple genesAuxiliary subunitsTwo-poreDiverse familyHuman diseasesMolecular biologyMessenger RNASubunitsDiversityGenesRectifier channelsVoltage-dependent sodiumChannel propertiesPatch-clamp techniqueSplicingDifferent mechanismsHeterotetramerClamp techniqueElectrophysiological measurementsRNA
1999
Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission
Jonas E, Buchanan J, Kaczmarek L. Prolonged Activation of Mitochondrial Conductances During Synaptic Transmission. Science 1999, 286: 1347-1350. PMID: 10558987, DOI: 10.1126/science.286.5443.1347.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsCalciumCalcium ChannelsDecapodiformesElectric ConductivityElectric StimulationIntracellular MembranesIon ChannelsIon TransportMicroscopy, ElectronMitochondriaPatch-Clamp TechniquesPorinsPresynaptic TerminalsSynaptic TransmissionTime FactorsVoltage-Dependent Anion ChannelsConceptsChannel activityIon channel activityMitochondrial membraneOnly organellesIntracellular organellesIntact cellsIon channelsMitochondriaOrganellesLarge conductanceTens of secondsPresynaptic terminalsIon transportSynaptic transmissionSynaptic stimulationConductanceElectron microscopyPatch-clamp techniqueMembraneActivityCellsActivationSquidStimulation
1997
Giga-Ohm Seals on Intracellular Membranes: A Technique for Studying Intracellular Ion Channels in Intact Cells
Jonas E, Knox R, Kaczmarek L. Giga-Ohm Seals on Intracellular Membranes: A Technique for Studying Intracellular Ion Channels in Intact Cells. Neuron 1997, 19: 7-13. PMID: 9247259, DOI: 10.1016/s0896-6273(00)80343-8.Peer-Reviewed Original ResearchConceptsGiga-ohm sealsIntracellular membranesIntact cellsIntracellular ion channelsMembrane ion channel activityIon channel activityConventional patch clampingOrganelle membranesPlasma membraneInternal organellesInternal membranesInternal organelles membraneCell typesIon channelsChannel activityConfocal imagingInositol trisphosphateLipophilic fluorescent dyeMembranePatch clampingConcentric electrode arrangementPatch-clamp techniqueCellsIntact neuronsOrganelles
1996
Inhibition by Nystatin of Kv1.3 Channels Expressed in Chinese Hamster Ovary Cells
HAHN S, WANG L, KACZMAREK L. Inhibition by Nystatin of Kv1.3 Channels Expressed in Chinese Hamster Ovary Cells. Neuropharmacology 1996, 35: 895-901. PMID: 8938720, DOI: 10.1016/0028-3908(96)00094-9.Peer-Reviewed Original ResearchConceptsKv1.3 currentsEffect of nystatinPerforated patch recordingsChinese hamster ovary cellsPatch-clamp techniqueWhole-cell configurationConcentration-dependent reductionHamster ovary cellsRectifier potassium channelHalf-maximal inhibitionPotassium channel Kv1.3Patch recordingsMaximal inhibitionAmphotericin BOvary cellsKv1.3 channelsPotassium channelsTetraethylammoniumChannel Kv1.3Intracellular solutionInhibitionRapid blockNystatinDrugsApparent inactivation
1990
Control of potassium currents and cyclic AMP levels by autoactive neuropeptides in Aplysia neurons
Loechner K, Kaczmarek L. Control of potassium currents and cyclic AMP levels by autoactive neuropeptides in Aplysia neurons. Brain Research 1990, 532: 1-6. PMID: 2178030, DOI: 10.1016/0006-8993(90)91733-w.Peer-Reviewed Original ResearchConceptsVoltage-dependent potassium currentsCyclic AMP levelsBag cell neuronsPotassium currentCell neuronsAMP levelsAlpha-BCPWhole-cell patch-clamp techniqueCell patch-clamp techniqueBag cell afterdischargeTransient voltage-dependent potassium currentPatch-clamp techniqueDelayed potassium currentPharmacologic elevationClamp techniqueOutward currentsAfterdischargesNeuronsCell peptidesAplysia neuronsNeuropeptidesBeta-BCPConsistent effectSecond messengerAlpha
1988
Inositol trisphosphate releases intracellularly stored calcium and modulates ion channels in molluscan neurons
Fink L, Connor J, Kaczmarek L. Inositol trisphosphate releases intracellularly stored calcium and modulates ion channels in molluscan neurons. Journal Of Neuroscience 1988, 8: 2544-2555. PMID: 2470874, PMCID: PMC6569530, DOI: 10.1523/jneurosci.08-07-02544.1988.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsIntracellular calciumAction potentialsFluorescent calcium indicator fura-2Inositol trisphosphateCalcium indicator fura-2Intracellular calcium levelsIndicator fura-2Non-neuronal cellsCell-attached patch-clamp techniquePatch-clamp techniqueMicroinjection of inositolCultured bag cell neuronsHydrolysis of phosphoinositidesCalcium-containing mediumElectrophysiological effectsInjection of inositolFura-2Calcium levelsExtracellular calciumIsolated neuronsMolluscan neuronsCytosolic calciumTransient hyperpolarization
1986
Multiple components of delayed potassium current in peptidergic neurons of Aplysia: modulation by an activator of adenylate cyclase
Strong J, Kaczmarek L. Multiple components of delayed potassium current in peptidergic neurons of Aplysia: modulation by an activator of adenylate cyclase. Journal Of Neuroscience 1986, 6: 814-822. PMID: 2420948, PMCID: PMC6568473, DOI: 10.1523/jneurosci.06-03-00814.1986.Peer-Reviewed Original ResearchConceptsAction potentialsApplication of forskolinBag cell neuronsPotassium currentCell neuronsOutward currentsWhole-cell patch-clamp techniqueBag cell afterdischargePatch-clamp techniqueEffect of forskolinElevation of cAMPPeptidergic neuronsCalcium entryClamp techniqueAfterdischargesReproductive tractMsec depolarizationsAdenylate cyclasePrimary culturesHigh intracellular concentrationsNeuronsMin periodRepetitive activitySpike widthForskolin