2017
An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons
Zhang Y, Ni W, Horwich AL, Kaczmarek LK. An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons. Journal Of Neuroscience 2017, 37: 2258-2265. PMID: 28119399, PMCID: PMC5338764, DOI: 10.1523/jneurosci.3102-16.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaBiophysicsCells, CulturedElectric StimulationEnzyme InhibitorsGanglia, InvertebrateHumansLuminescent ProteinsMembrane PotentialsMicroinjectionsMorpholinosMutationNerve Tissue ProteinsNeuronsPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Sodium-ActivatedRNA, Small InterferingSodiumSuperoxide Dismutase-1ConceptsAmyotrophic lateral sclerosisSuperoxide dismutase 1Mutant superoxide dismutase 1Potassium currentC-Jun N-terminal kinaseNeuronal excitabilityLateral sclerosisFatal adult-onset neurodegenerative diseaseN-terminal kinaseMutant human Cu/ZnNeuronal developmentDismutase 1Adult-onset neurodegenerative diseaseCurrent-clamp recordingsMotor neuron toxicityOutward potassium currentHuman Cu/ZnWild-type superoxide dismutase 1Neuron toxicityActivity of NaBag cell neuronsClamp recordingsNeuronal functionCell neuronsAction potentials
2012
Regulation of Neuronal Excitability by Interaction of Fragile X Mental Retardation Protein with Slack Potassium Channels
Zhang Y, Brown MR, Hyland C, Chen Y, Kronengold J, Fleming MR, Kohn AB, Moroz LL, Kaczmarek LK. Regulation of Neuronal Excitability by Interaction of Fragile X Mental Retardation Protein with Slack Potassium Channels. Journal Of Neuroscience 2012, 32: 15318-15327. PMID: 23115170, PMCID: PMC3518385, DOI: 10.1523/jneurosci.2162-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnisomycinAplysiaCHO CellsCloning, MolecularCricetinaeCricetulusElectrophysiological PhenomenaFragile X Mental Retardation ProteinImmunohistochemistryImmunoprecipitationNeuronsPatch-Clamp TechniquesPotassium ChannelsProtein Synthesis InhibitorsRNA InterferenceRNA, Small InterferingSodiumSynapsesConceptsNeuronal excitabilitySlack potassium channelsTetrodotoxin-sensitive componentCurrent-clamp recordingsSlack channelsMental retardation proteinBag cell neuronsSustained componentIntracellular injectionNeuronal firingInhibitory periodSynaptic stimulationPotassium currentCell neuronsAction potentialsOutward currentsPotassium channelsProlonged changesNeuronsAplysia bag cell neuronsProtein synthesis inhibitor anisomycinExcitabilityFragile X Mental Retardation ProteinCommon formIntellectual disability
2008
Repetitive Firing Triggers Clustering of Kv2.1 Potassium Channels in Aplysia Neurons*
Zhang Y, McKay SE, Bewley B, Kaczmarek LK. Repetitive Firing Triggers Clustering of Kv2.1 Potassium Channels in Aplysia Neurons*. Journal Of Biological Chemistry 2008, 283: 10632-10641. PMID: 18276591, DOI: 10.1074/jbc.m800253200.Peer-Reviewed Original ResearchConceptsBag cell neuronsKv2.1 channelsPotassium channelsPlasma membraneC-terminusKv2.1 clustersKv2.1 potassium channelCell neuronsMammalian neuronsReproductive behaviorRectifier potassium channelFrequency-dependent broadeningRapid redistributionAplysia neuronsClamp recordingsAcid peptidePartial inactivationRing-like clustersPhysiological changesKv2.1Neuronal excitabilityCentral nervous systemAction potentialsGenesNervous system
2006
A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia
Kachoei BA, Knox RJ, Uthuza D, Levy S, Kaczmarek LK, Magoski NS. A Store-Operated Ca2+ Influx Pathway in the Bag Cell Neurons of Aplysia. Journal Of Neurophysiology 2006, 96: 2688-2698. PMID: 16885525, PMCID: PMC2894935, DOI: 10.1152/jn.00118.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnilidesAnimalsAplysiaCalcium Channel BlockersCalcium ChannelsCalcium SignalingElectrophysiologyEnzyme InhibitorsImidazolesIn Vitro TechniquesIndicators and ReagentsIndolesInositol 1,4,5-TrisphosphateLanthanumMacrocyclic CompoundsMembrane PotentialsNeuronsNickelOxazolesRyanodine Receptor Calcium Release ChannelThapsigarginThiadiazolesConceptsBag cell neuronsCell neuronsCyclopiazonic acidCPA-sensitive storesSmooth muscle cell lineAbsence of extracellularCultured bag cell neuronsRyanodine receptor agonistATPase inhibitorReceptor blockersReceptor agonistNeuropeptide secretionXestospongin CMuscle cell lineInflux pathwayIntracellular storesNonneuronal cellsAcidic storesElevated intracellularNeuronsStore depletionAfterdischargesCell linesBafilomycin AEntry pathway
2005
Association/Dissociation of a Channel–Kinase Complex Underlies State-Dependent Modulation
Magoski NS, Kaczmarek LK. Association/Dissociation of a Channel–Kinase Complex Underlies State-Dependent Modulation. Journal Of Neuroscience 2005, 25: 8037-8047. PMID: 16135761, PMCID: PMC2873328, DOI: 10.1523/jneurosci.1903-05.2005.Peer-Reviewed Original ResearchConceptsProtein kinase CSrc homology 3 domainCation channelsSrc tyrosine kinasePKC-dependent modulationPhorbol esterSrc-dependent regulationAplysia bag cell neuronsBag cell neuronsProtein kinaseAssociated kinaseAssociation/dissociationEgg-laying hormonePhosphotyrosine stainingTyrosine kinaseKinase CKinaseReproductive behaviorNonselective cation channelsIon channelsChannel activityUnstimulated neuronsDependent modulationCell neuronsLong-term excitability
2004
The Appearance of a Protein Kinase A-regulated Splice Isoform of slo Is Associated with the Maturation of Neurons That Control Reproductive Behavior*
Zhang Y, Joiner WJ, Bhattacharjee A, Rassendren F, Magoski NS, Kaczmarek LK. The Appearance of a Protein Kinase A-regulated Splice Isoform of slo Is Associated with the Maturation of Neurons That Control Reproductive Behavior*. Journal Of Biological Chemistry 2004, 279: 52324-52330. PMID: 15375169, DOI: 10.1074/jbc.m408543200.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAmino Acid SequenceAnimalsAplysiaCell DifferentiationCHO CellsCricetinaeCyclic AMP-Dependent Protein KinasesDNA, ComplementaryIn Vitro TechniquesLarge-Conductance Calcium-Activated Potassium ChannelsMolecular Sequence DataNeuronsPatch-Clamp TechniquesPotassium Channels, Calcium-ActivatedProtein IsoformsRecombinant ProteinsReproductionConceptsBag cell neuronsReproductive behaviorSlo geneConsensus phosphorylation sitesCell cDNA libraryProtein kinase ACell neuronsChinese hamster ovary cellsPhosphorylation sitesCatalytic subunitHamster ovary cellsAlternative transcriptsCDNA librarySplice isoformsKinase ABK channel activityMaturation of neuronsPKA inhibitorVoltage-dependent channelsOvary cellsBrief synaptic stimulationChannel activityMature neuronsIsoformsPKAActivation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia
Knox RJ, Magoski NS, Wing D, Barbee SJ, Kaczmarek LK. Activation of a calcium entry pathway by sodium pyrithione in the bag cell neurons of Aplysia. Developmental Neurobiology 2004, 60: 411-423. PMID: 15307146, DOI: 10.1002/neu.20029.Peer-Reviewed Original ResearchConceptsAplysia bag cell neuronsWhole-cell current-clamp recordingsBag cell neuronsPlasma membraneCurrent-clamp recordingsNeuronal physiologyCytosolic pHCytosolic freeMembrane potentialCell neuronsSodium pyrithionePresence of externalRatiometric imagingMV depolarizationClose structural analogueHill coefficientNapStructural analoguesSpecies
2002
Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons
Zhang Y, Magoski NS, Kaczmarek LK. Prolonged Activation of Ca2+-Activated K+Current Contributes to the Long-Lasting Refractory Period ofAplysia Bag Cell Neurons. Journal Of Neuroscience 2002, 22: 10134-10141. PMID: 12451114, PMCID: PMC6758731, DOI: 10.1523/jneurosci.22-23-10134.2002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaCalciumCells, CulturedElectric StimulationEnzyme ActivatorsEnzyme InhibitorsLarge-Conductance Calcium-Activated Potassium ChannelsNeural InhibitionNeuronsPatch-Clamp TechniquesPhloretinPotassiumPotassium Channel BlockersPotassium Channels, Calcium-ActivatedProtein Kinase CRefractory Period, ElectrophysiologicalSphingosineTetradecanoylphorbol AcetateConceptsBag cell neuronsCell neuronsRefractory periodBK currentsProtein kinase COnset of afterdischargeBK channel activityApplication of phloretinBK channel activatorsProlonged refractory periodAbility of stimulationRole of Ca2Blocker paxillinePharmacological characteristicsChannel activatorIntracellular Ca2Prolonged increaseOutward currentsInhibitor of PKCAfterdischargesNeuronsAdditional stimulationProlonged activationActivator of PKCChannel activity
2001
Aplysia Ror Forms Clusters on the Surface of Identified Neuroendocrine Cells
McKay S, Hislop J, Scott D, Bulloch A, Kaczmarek L, Carew T, Sossin W. Aplysia Ror Forms Clusters on the Surface of Identified Neuroendocrine Cells. Molecular And Cellular Neuroscience 2001, 17: 821-841. PMID: 11358481, DOI: 10.1006/mcne.2001.0977.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAmino Acid SequenceAnimalsAntibody SpecificityAplysiaBase SequenceCaenorhabditis elegans ProteinsCell CompartmentationCells, CulturedCloning, MolecularGanglia, InvertebrateImmunohistochemistryMolecular Sequence DataNeuronsNeurosecretory SystemsReceptor Protein-Tyrosine KinasesReceptor Tyrosine Kinase-like Orphan ReceptorsReceptors, Cell SurfaceRNA, MessengerConceptsBag cell neuronsNeuroendocrine bag cell neuronsROR receptorsCultured bag cell neuronsRegulation of growthReceptor tyrosine kinasesMarine mollusk Aplysia californicaPeripheral neuronal processesMollusk Aplysia californicaCellular polarityFunctional domainsTyrosine kinaseIntracellular organellesCell surfaceProteinNeuroendocrine cellsKinaseAplysia californicaRelease sitesNeuronal processesOrganellesNeuronal populationsForm clustersGanglionic neuropilReceptors
1998
Expression of a foreign G-protein coupled receptor modulates the excitability of the peptidergic bag cell neurons of Aplysia
Whim M, Kaczmarek L. Expression of a foreign G-protein coupled receptor modulates the excitability of the peptidergic bag cell neurons of Aplysia. Neuroscience Letters 1998, 258: 143-146. PMID: 9885951, DOI: 10.1016/s0304-3940(98)00850-7.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsMetabotropic glutamate receptorsG proteinsPeptidergic bag cell neuronsAfferent stimulationSpontaneous firingAfferent inputGlutamate receptorsSecond messenger pathwaysPharmacological activationReceptor activationAfterdischargesNeuronsMessenger pathwaysReceptorsActivationAdditional pathwaysExpressionExcitabilityPathwaySustained periodHeterologous Expression of the Kv3.1 Potassium Channel Eliminates Spike Broadening and the Induction of a Depolarizing Afterpotential in the Peptidergic Bag Cell Neurons
Whim M, Kaczmarek L. Heterologous Expression of the Kv3.1 Potassium Channel Eliminates Spike Broadening and the Induction of a Depolarizing Afterpotential in the Peptidergic Bag Cell Neurons. Journal Of Neuroscience 1998, 18: 9171-9180. PMID: 9801357, PMCID: PMC6792887, DOI: 10.1523/jneurosci.18-22-09171.1998.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsAction potentialsCalcium entryUse-dependent inactivationExpression of Kv3.1Kv3.1 potassium channelPeptidergic bag cell neuronsControl neuronsSpontaneous firingBrief synaptic stimulationCalcium currentNeuronal excitabilityIntracellular calciumCalcium influxSynaptic stimulationDepolarizing afterpotentialsBAPTA-AMAfterpotentialsNeuronsPotassium channelsClusters of cellsKv3.1InductionPhysiological roleProtein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia
White B, Nick T, Carew T, Kaczmarek L. Protein Kinase C Regulates a Vesicular Class of Calcium Channels in the Bag Cell Neurons of Aplysia. Journal Of Neurophysiology 1998, 80: 2514-2520. PMID: 9819259, DOI: 10.1152/jn.1998.80.5.2514.Peer-Reviewed Original ResearchModulation of a calcium-sensitive nonspecific cation channel by closely associated protein kinase and phosphatase activities
Wilson G, Magoski N, Kaczmarek L. Modulation of a calcium-sensitive nonspecific cation channel by closely associated protein kinase and phosphatase activities. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10938-10943. PMID: 9724808, PMCID: PMC27999, DOI: 10.1073/pnas.95.18.10938.Peer-Reviewed Original ResearchConceptsProtein kinaseCation channelsProtein phosphatase 1Protein tyrosine phosphataseNonspecific cation channelProtein kinase C inhibitorPresence of H7Nonhydrolyzable ATP analogKinase C inhibitorRegulatory complexPhosphatase 1Bag cell neuronsTyrosine phosphataseExcised patchesOpen probabilityCytoplasmic sideMolecular switchATP analogC inhibitorPhosphatase activityKinaseChannel closureSpontaneous action potentialsPatch-clamp studiesATP
1997
Identification of a Vesicular Pool of Calcium Channels in the Bag Cell Neurons of Aplysia californica
White B, Kaczmarek L. Identification of a Vesicular Pool of Calcium Channels in the Bag Cell Neurons of Aplysia californica. Journal Of Neuroscience 1997, 17: 1582-1595. PMID: 9030618, PMCID: PMC6573390, DOI: 10.1523/jneurosci.17-05-01582.1997.Peer-Reviewed Original ResearchConceptsBag cell neuronsCalcium channel alpha1 subunitAplysia nervous systemProtein kinase CCell neuronsAplysia californicaBag cell clustersCalcium channelsChannel alpha1 subunitCell clustersVesicular channelsMembrane proteinsReverse-transcribed RNAVesicular localizationPlasma membraneEgg-laying hormoneMolecular mechanismsSubcellular distributionKinase CLysoTracker RedDense-core vesiclesAcidic organellesGrowth conesCalcium channel subtypesCalcium current modulationRegulation by insulin of a unique neuronal Ca2+ pool and of neuropeptide secretion
Jonas E, Knox R, Smith T, Wayne N, Connor J, Kaczmarek L. Regulation by insulin of a unique neuronal Ca2+ pool and of neuropeptide secretion. Nature 1997, 385: 343-346. PMID: 9002519, DOI: 10.1038/385343a0.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzoquinonesCalciumCalcium ChannelsCells, CulturedCyclic AMPEndoplasmic ReticulumHeparinInositol 1,4,5-TrisphosphateInositol 1,4,5-Trisphosphate ReceptorsInsulinInvertebrate HormonesLactams, MacrocyclicNeuronsNeuropeptidesProtein-Tyrosine KinasesQuinonesReceptors, Cytoplasmic and NuclearRifabutinThapsigarginConceptsIntracellular Ca2Neuropeptide secretionSpontaneous action potentialsEffect of insulinSecretion of neuropeptidesTyrosine kinase receptorsAcute riseBag cell neuronsDistal tipNeuronal dischargeNeuronal Ca2Distinct intracellular poolsCell neuronsAction potentialsCyclic AMP analogueInsulinNeuropeptidesInsulin receptorKinase receptorsSecretionPresumed siteNeuronsIntracellular poolMitochondrial Ca2Receptors
1996
Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons
Nick T, Kaczmarek L, Carew T. Ionic Currents Underlying Developmental Regulation of Repetitive Firing in Aplysia Bag Cell Neurons. Journal Of Neuroscience 1996, 16: 7583-7598. PMID: 8922415, PMCID: PMC6579091, DOI: 10.1523/jneurosci.16-23-07583.1996.Peer-Reviewed Original ResearchConceptsBag cell neuronsRepetitive firingCell neuronsEgg-laying behaviorJuvenile neuronsTetraethyl ammonium chlorideDevelopmental regulationSpecific ionic currentsAplysia bag cell neuronsAfterdischargesElectrical stimulationVariety of stimuliDevelopmental controlNeuronal systemsProlonged depolarizationBehavioral effectsNeuronsIonic currentsDevelopmental mechanismsSelective regulationAdultsAplysia californicaFiringRegulationDepolarizationDevelopmental dissociation of excitability and secretory ability in Aplysia bag cell neurons
Nick T, Moreira J, Kaczmarek L, Carew T, Wayne N. Developmental dissociation of excitability and secretory ability in Aplysia bag cell neurons. Journal Of Neurophysiology 1996, 76: 3351-3359. PMID: 8930278, DOI: 10.1152/jn.1996.76.5.3351.Peer-Reviewed Original ResearchConceptsBag cell neuronsEgg-laying behaviorCell neuronsPotential functional significanceAplysia bag cell neuronsDevelopmental regulationVesicular localizationSecretory vesiclesJuvenile AplysiaPremature expressionImmature neuronsDownstream elementsMature formBody sizeReproductive behaviorImmunoelectron microscopyPotassium channel blocker tetraethylammoniumLarge vesiclesDevelopmental interactionsFunctional significanceBag cell hormonesVesiclesCessation of locomotionMature neuronsElectrical activityCa2+ influx and activation of a cation current are coupled to intracellular Ca2+ release in peptidergic neurons of Aplysia californica.
Knox RJ, Jonas EA, Kao LS, Smith PJ, Connor JA, Kaczmarek LK. Ca2+ influx and activation of a cation current are coupled to intracellular Ca2+ release in peptidergic neurons of Aplysia californica. The Journal Of Physiology 1996, 494: 627-639. PMID: 8865062, PMCID: PMC1160665, DOI: 10.1113/jphysiol.1996.sp021520.Peer-Reviewed Original ResearchConceptsBag cell neuronsCell neuronsThapsigargin-sensitive Ca2Cation currentReversal potentialVoltage-activated Ba2Non-selective cation currentAplysia californicaApparent reversal potentialSteady-state Ca2Thapsigargin-induced elevationMin. 3Endoplasmic reticulum Ca2Voltage-clamp experimentsMicroM tetrodotoxinPeptidergic neuronsIntact gangliaAbdominal ganglionExtracellular Ca2Intracellular Ca2Intracellular storesBAPTA-AMSmall depolarizationBasal levelsNeuronsIdentification and Characterization of a Ca2+-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons
Wilson G, Richardson F, Fisher T, Olivera B, Kaczmarek L. Identification and Characterization of a Ca2+-Sensitive Nonspecific Cation Channel Underlying Prolonged Repetitive Firing in Aplysia Neurons. Journal Of Neuroscience 1996, 16: 3661-3671. PMID: 8642410, PMCID: PMC6578840, DOI: 10.1523/jneurosci.16-11-03661.1996.Peer-Reviewed Original ResearchConceptsNonspecific cation channelRepetitive firingCation channelsSpontaneous repetitive firingNeuronal response propertiesBag cell neuronsSlow inwardNeuronal excitabilityCell neuronsChannels underliesProlonged dischargeAplysia bag cell neuronsAplysia neuronsAfterdischargesNeuronsPhosphorylation-mediated changesResponse propertiesChannel recordingsConus textileFiringO mVTetrodotoxinExcitabilityInsulin receptor in Aplysia neurons: characterization, molecular cloning, and modulation of ion currents
Jonas E, Knox R, Kaczmarek L, Schwartz J, Solomon D. Insulin receptor in Aplysia neurons: characterization, molecular cloning, and modulation of ion currents. Journal Of Neuroscience 1996, 16: 1645-1658. PMID: 8774433, PMCID: PMC6578688, DOI: 10.1523/jneurosci.16-05-01645.1996.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino Acid SequenceAnimalsAplysiaBase SequenceCalcium ChannelsCloning, MolecularDNA, ComplementaryElectrophysiologyImmunohistochemistryInsulinIon ChannelsMolecular ProbesMolecular Sequence DataNeuronsPotassium ChannelsProtein-Tyrosine KinasesReceptor, InsulinTissue DistributionConceptsBag cell neuronsInsulin receptorInsulin-like peptidesImmunocytochemical staining showCell neuronsTyrosine kinase receptorsVertebrate insulinsMolecular cloningHerbimycin ATyrosine residuesTyrosine kinaseKinase receptorsInsulin-like growth factor-1Factor 1Staining showsVoltage-clamped neuronsVoltage-dependent Ca2Growth factor-1Aplysia californicaAplysia neuronsNervous systemReceptorsAction potentialsNeuronsInsulin