2024
Engineered vascular grafts lend unique insight to pathophysiology of aortic aneurysms
Naegeli K, Niklason L. Engineered vascular grafts lend unique insight to pathophysiology of aortic aneurysms. Cell Stem Cell 2024, 31: 1099-1100. PMID: 39094540, DOI: 10.1016/j.stem.2024.07.002.Peer-Reviewed Original ResearchMechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis
Kural M, Djakbarova U, Cakir B, Tanaka Y, Chan E, Arteaga Muniz V, Madraki Y, Qian H, Park J, Sewanan L, Park I, Niklason L, Kural C. Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis. Cell Death & Disease 2024, 15: 440. PMID: 38909035, PMCID: PMC11193792, DOI: 10.1038/s41419-024-06822-3.Peer-Reviewed Original ResearchConceptsFas-induced apoptosisCell surface Fas expressionDeath receptor FasInhibition of endocytosisSurface Fas expressionPlasma membrane tensionCancer cell apoptosisEndocytosis dynamicsApoptotic signalingReceptor FasGlioblastoma cell growthFas expressionPlasma membraneCell growthEndocytosisXenograft mouse modelSoluble FasLCell apoptosisFasApoptosisRho-kinase inhibitorCancer cellsMembrane tensionNonmalignant cellsInduce tumor regression335-OR: Preliminary Results of Islet Survival in a Novel Biovascular Pancreas Implanted in Primates
KURAL M, NASH K, NAEGELI K, QIAN H, WANG J, KIRKTON R, NIKLASON L. 335-OR: Preliminary Results of Islet Survival in a Novel Biovascular Pancreas Implanted in Primates. Diabetes 2024, 73 DOI: 10.2337/db24-335-or.Peer-Reviewed Original ResearchUse of the Human Acellular Vessel to Repair Iatrogenic Vascular Trauma
Lum Y, Moore E, Morrison J, Shores J, Niklason L, Parikh S. Use of the Human Acellular Vessel to Repair Iatrogenic Vascular Trauma. Journal Of Vascular Surgery 2024, 79: e297. DOI: 10.1016/j.jvs.2024.03.385.Peer-Reviewed Original ResearchCharacteristics of Hemodialysis Patients Experiencing Vascular Access Difficulties
Niklason L, Panaccio M, Willetts J. Characteristics of Hemodialysis Patients Experiencing Vascular Access Difficulties. Journal Of Vascular Surgery 2024, 79: e179. DOI: 10.1016/j.jvs.2024.03.226.Peer-Reviewed Original ResearchResistance to Infection of the Human Acellular Vessel in Extremity Arterial Trauma Repair
Moore E, Fox C, Niklason L, Parikh S. Resistance to Infection of the Human Acellular Vessel in Extremity Arterial Trauma Repair. Journal Of Vascular Surgery 2024, 79: e155. DOI: 10.1016/j.jvs.2024.03.189.Peer-Reviewed Original Research#2598 Risk of vascular access complications in haemodialysis patients initiating dialysis with arteriovenous fistula or arteriovenous graft
Willetts J, Usvyat L, Chaudhuri S, Niklason L, Maddux F. #2598 Risk of vascular access complications in haemodialysis patients initiating dialysis with arteriovenous fistula or arteriovenous graft. Nephrology Dialysis Transplantation 2024, 39: gfae069-1550-2598. DOI: 10.1093/ndt/gfae069.1550.Peer-Reviewed Original ResearchVascular access complication ratesArteriovenous fistulaEnd-stage kidney diseaseArteriovenous graftsVA complicationsVascular accessIn-center hemodialysisInitial VAComorbid conditionsDuration of follow-upAssociated with increased riskComplication eventsHistory of myocardial infarctionCongestive heart failureVascular access complicationsDiabetic ptsRisk of vascular access complicationsComplication rateDemographic characteristicsIncreased BMIDocumented thrombosisIn-center hemodialysis patientsContinuous hemodialysisHigher BMIHaemodialysis patientsSingle-cell RNA-seq analysis of cell-cell communications in human lung reveals a novel role of VEGF-D in acute lung injury
Yuan Y, Sharma L, Tang W, Raredon M, Ahangari F, Khoury J, Wu D, Niklason L, Kaminski N. Single-cell RNA-seq analysis of cell-cell communications in human lung reveals a novel role of VEGF-D in acute lung injury. Physiology 2024, 39: 1314. DOI: 10.1152/physiol.2024.39.s1.1314.Peer-Reviewed Original ResearchIdiopathic pulmonary fibrosisAcute lung injuryChronic obstructive pulmonary diseaseAcute respiratory distress syndromeAnalysis of cell-cell communicationVEGF-DMicrovascular nicheSingle-cell RNA-seqLung injury modelSingle-cell RNA-seq analysisLung injuryCell-cell communicationLigand-receptor pairsLPS-induced lung injury modelRNA-seqAdjacent cell typesPulmonary diseaseInjury modelHuman lung endothelial cellsBarrier functionImmune cell infiltrationTumor necrosis factor-aRespiratory distress syndromeLung vascular integrityGene expressionVEGF-D Is Enriched in the Human Lung Microvascular Niche and Improves Vascular Integrity During Acute Lung Injury
Yuan Y, Sharma L, Tang W, Raredon M, Ahangari F, Khoury J, Wu D, Niklason L, Kaminski N. VEGF-D Is Enriched in the Human Lung Microvascular Niche and Improves Vascular Integrity During Acute Lung Injury. 2024, a4694-a4694. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a4694.Peer-Reviewed Original ResearchPhase 2/3 Study for the Evaluation of Safety and Efficacy of HAV for Vascular Reconstruction in Patients With Limb- or Life-threatening Vascular Trauma
Moore E, Fox C, Curi M, Kundi R, Lum Y, Namias N, Rajani R, Niklason L, Parikh S. Phase 2/3 Study for the Evaluation of Safety and Efficacy of HAV for Vascular Reconstruction in Patients With Limb- or Life-threatening Vascular Trauma. Journal Of Vascular Surgery 2024, 79: 45s-46s. DOI: 10.1016/j.jvs.2024.01.110.Peer-Reviewed Original Research
2023
Clinical and Demographic Characteristics Associated with Arteriove-nous Access-Related Infections in US Patients Initiating Hemodialysis
Pulgar S, Panaccio M, Willetts J, Hussain M, Chaudhuri S, Usvyat L, Niklason L. Clinical and Demographic Characteristics Associated with Arteriove-nous Access-Related Infections in US Patients Initiating Hemodialysis. Journal Of The American Society Of Nephrology 2023, 34: 536-536. DOI: 10.1681/asn.20233411s1536b.Peer-Reviewed Original Research
2021
Five-Year Outcome in Patients with ESRD Who Received the Bioengineered Human Acellular Vessel for Dialysis Access
Lawson J, Niklason L, Pilgrim A, Kirkton R, Prichard H. Five-Year Outcome in Patients with ESRD Who Received the Bioengineered Human Acellular Vessel for Dialysis Access. Journal Of The American Society Of Nephrology 2021, 32: 232-232. DOI: 10.1681/asn.20213210s1232b.Peer-Reviewed Original Research
2016
Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices
Calle EA, Hill RC, Leiby KL, Le AV, Gard AL, Madri JA, Hansen KC, Niklason LE. Targeted proteomics effectively quantifies differences between native lung and detergent-decellularized lung extracellular matrices. Acta Biomaterialia 2016, 46: 91-100. PMID: 27693690, PMCID: PMC5451113, DOI: 10.1016/j.actbio.2016.09.043.Peer-Reviewed Original ResearchConceptsExtracellular matrixLung extracellular matrixMatrix proteinsECM-associated proteinsCell-matrix interactionsProtein extraction methodsWhole organ regenerationRegenerative medicineOrganotypic cell culturesQuantitative proteomicsAcellular extracellular matrixECM proteinsOrgan regenerationCell adhesionProteomicsProtein analysisQuantitative biochemical dataProteinPotent substrateXenogeneic extracellular matrixTargeted proteomicsCell nuclear antigenBiochemical dataImportant glycoproteinCell culturesEngineered Tissue–Stent Biocomposites as Tracheal Replacements
Zhao L, Sundaram S, Le AV, Huang AH, Zhang J, Hatachi G, Beloiartsev A, Caty MG, Yi T, Leiby K, Gard A, Kural MH, Gui L, Rocco KA, Sivarapatna A, Calle E, Greaney A, Urbani L, Maghsoudlou P, Burns A, DeCoppi P, Niklason LE. Engineered Tissue–Stent Biocomposites as Tracheal Replacements. Tissue Engineering Part A 2016, 22: 1086-1097. PMID: 27520928, PMCID: PMC5312617, DOI: 10.1089/ten.tea.2016.0132.Peer-Reviewed Original ResearchImpaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice
Kristofik N, Calabro NE, Tian W, Meng A, MacLauchlan S, Wang Y, Breuer CK, Tellides G, Niklason LE, Kyriakides TR. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 2016, 128: 1642-1650. PMID: 27471233, PMCID: PMC5034742, DOI: 10.1182/blood-2016-03-702845.Peer-Reviewed Original ResearchNew Functional Tools for Antithrombogenic Activity Assessment of Live Surface Glycocalyx
Dimitrievska S, Gui L, Weyers A, Lin T, Cai C, Wu W, Tuggle CT, Sundaram S, Balestrini JL, Slattery D, Tchouta L, Kyriakides TR, Tarbell JM, Linhardt RJ, Niklason LE. New Functional Tools for Antithrombogenic Activity Assessment of Live Surface Glycocalyx. Arteriosclerosis Thrombosis And Vascular Biology 2016, 36: 1847-1853. PMID: 27386939, PMCID: PMC5283952, DOI: 10.1161/atvbaha.116.308023.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntithrombinsAorta, ThoracicBiological AssayBlood CoagulationCells, CulturedChromatography, LiquidFactor XaGlycocalyxHeparinHeparitin SulfateHuman Umbilical Vein Endothelial CellsMaleMass SpectrometryMicroscopy, Electron, TransmissionRats, Sprague-DawleyReproducibility of ResultsThrombinTime FactorsConceptsHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVein endothelial cellsNative aortaEndothelial cellsHuman umbilical vein endothelial cell monolayersCultured human umbilical vein endothelial cellsFactor XaVascular healthEndothelial cell monolayersVascular diseaseRat aortaDamaged vasculatureAnticoagulant capacityVascular communityAortaTherapeutic developmentActivity assessmentCell monolayersVascular graftsLiquid chromatography-mass spectrometry analysisNovel assayGlycocalyxAssaysNative rat aortaComparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine
Balestrini JL, Gard AL, Gerhold KA, Wilcox EC, Liu A, Schwan J, Le AV, Baevova P, Dimitrievska S, Zhao L, Sundaram S, Sun H, Rittié L, Dyal R, Broekelmann TJ, Mecham RP, Schwartz MA, Niklason LE, White ES. Comparative biology of decellularized lung matrix: Implications of species mismatch in regenerative medicine. Biomaterials 2016, 102: 220-230. PMID: 27344365, PMCID: PMC4939101, DOI: 10.1016/j.biomaterials.2016.06.025.Peer-Reviewed Original ResearchConceptsHuman endothelial cellsCell-matrix interactionsLung regenerationEndothelial cellsKey matrix proteinsComparative biologyCell adhesion moleculeMatrix proteinsLung extracellular matrixCell healthExtracellular matrixResidual DNASpecies mismatchRat lung scaffoldsRegenerative medicineAdhesion moleculesLung scaffoldsPrimate tissuesCellsVascular cell adhesion moleculeLung engineeringLung matrixLess expressionPulmonary cellsProfound effectImplantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraftBiaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries
Huang AH, Balestrini JL, Udelsman BV, Zhou KC, Zhao L, Ferruzzi J, Starcher BC, Levene MJ, Humphrey JD, Niklason LE. Biaxial Stretch Improves Elastic Fiber Maturation, Collagen Arrangement, and Mechanical Properties in Engineered Arteries. Tissue Engineering Part C Methods 2016, 22: 524-533. PMID: 27108525, PMCID: PMC4921901, DOI: 10.1089/ten.tec.2015.0309.Peer-Reviewed Original ResearchConceptsTissue-engineered blood vesselsBiaxial loadingMechanical propertiesMechanical strengthFiber orientationMultiaxial loadingLoading conditionsMechanical integrityBiaxial stretchingCollagen undulationArtificial skinNovel bioreactorMechanical failureMatrix orientationBiaxial stretchLoadingAxial stretchCollagen fiber orientationSuture strengthNative arteriesTissue equivalentsStrengthPropertiesCircumferential stretchMatrix contentBioengineered human acellular vessels for dialysis access in patients with end-stage renal disease: two phase 2 single-arm trials
Lawson JH, Glickman MH, Ilzecki M, Jakimowicz T, Jaroszynski A, Peden EK, Pilgrim AJ, Prichard HL, Guziewicz M, Przywara S, Szmidt J, Turek J, Witkiewicz W, Zapotoczny N, Zubilewicz T, Niklason LE. Bioengineered human acellular vessels for dialysis access in patients with end-stage renal disease: two phase 2 single-arm trials. The Lancet 2016, 387: 2026-2034. PMID: 27203778, PMCID: PMC4915925, DOI: 10.1016/s0140-6736(16)00557-2.Peer-Reviewed Original ResearchConceptsHuman acellular vesselEnd-stage renal diseaseAcellular vesselsPrimary patencySecondary patencyRenal diseaseDialysis accessHaemodialysis accessPhase 2 single-arm trialSingle-arm phase 2 trialPolytetrafluoroethylene arteriovenous graftsPhase 2 trialDialysis access graftsSingle-arm trialArms of patientsPrimary endpointChronic haemodialysisAccess graftsUS National InstitutesArteriovenous graftsVenous anastomosisCensoring eventIntimal hyperplasiaPatencyPatients