2011
Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture
Miyazawa K, Emmerling K, Manuelidis L. Replication and spread of CJD, kuru and scrapie agents in vivo and in cell culture. Virulence 2011, 2: 188-199. PMID: 21527829, PMCID: PMC3149681, DOI: 10.4161/viru.2.3.15880.Peer-Reviewed Original ResearchConceptsGT1 cellsSporadic CJDTSE agentsScrapie agentAgent-specific patternsTransmissible spongiform encephalopathy agentsComplex innate immune responseSpongiform encephalopathy agentInnate immune responseHost prion proteinK scrapie agentHuman CJDCJD agentNeuropathological sequelaeBrain titersImmune responseHuman kuruClearance mechanismsCJDInhibitory effectEnvironmental agentsCell-based assaysKuruInfectious formDistinct incubation times
2009
The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents
Manuelidis L, Chakrabarty T, Miyazawa K, Nduom NA, Emmerling K. The kuru infectious agent is a unique geographic isolate distinct from Creutzfeldt–Jakob disease and scrapie agents. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 13529-13534. PMID: 19633190, PMCID: PMC2715327, DOI: 10.1073/pnas.0905825106.Peer-Reviewed Original ResearchConceptsSporadic Creutzfeldt-Jakob diseaseCreutzfeldt-Jakob diseaseBovine spongiform encephalopathyBSE agentHuman sporadic Creutzfeldt-Jakob diseaseInfectious agentsEpidemic bovine spongiform encephalopathyTSE agentsScrapie agentTransmissible spongiform encephalopathy agentsSpongiform encephalopathy agentKuru agentLymphoreticular involvementBrain neuropathologySporadic CJDGT1 cellsNormal miceInfected humansNeurodegenerative diseasesDiseaseViral receptorsInfectious neurodegenerative diseasesSheep scrapieSpongiform encephalopathiesHigh levels
2008
Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein
Manuelidis L, Liu Y, Mullins B. Strain‐specific viral properties of variant Creutzfeldt–Jakob disease (vCJD) are encoded by the agent and not by host prion protein. Journal Of Cellular Biochemistry 2008, 106: 220-231. PMID: 19097123, PMCID: PMC2762821, DOI: 10.1002/jcb.21988.Peer-Reviewed Original ResearchConceptsVariant Creutzfeldt-Jakob diseaseBovine spongiform encephalopathyTransmissible spongiform encephalopathiesVCJD agentTSE strainsInfectious agentsNeuronal culturesEpidemic bovine spongiform encephalopathyMost viral infectionsCreutzfeldt-Jakob diseaseSpongiform encephalopathiesHost prion proteinHost PrP.Human CJDRegional neuropathologyVCJD brainPrion proteinSheep scrapie agentVariant CJDStrain-specific characteristicsBSE strainBrain homogenatesViral infectionPrimate brainScrapie agent
1983
Scrapie-associated fibrils in Creutzfeldt–Jakob disease
Merz P, Somerville R, Wisniewski H, Manuelidis L, Manuelidis E. Scrapie-associated fibrils in Creutzfeldt–Jakob disease. Nature 1983, 306: 474-476. PMID: 6358899, DOI: 10.1038/306474a0.Peer-Reviewed Original ResearchConceptsScrapie associated fibrilsCreutzfeldt-Jakob diseaseCentral nervous systemTitre of infectivityScrapie of sheepInfected brainPathological responseProgressive degenerationNervous systemSynaptosomal preparationsInfectious agentsBrain fractionsTransmissible encephalopathiesDiseaseHuman casesSpleen extractsAbnormal fibrilsSusceptible hostsScrapieClose associationDifferent tissuesEncephalopathyAgentsDegenerationTitres