Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation
Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Journal Of Cell Biology 2022, 221: e202207022. PMID: 36282247, PMCID: PMC9597353, DOI: 10.1083/jcb.202207022.Peer-Reviewed Original ResearchMeSH KeywordsCarrier ProteinsFatty AcidsGlycerophospholipidsLipid DropletsLipid MetabolismMitochondriaMitochondrial ProteinsConceptsEndoplasmic reticulumLipid dropletsProtein-mediated transferLipid transport proteinsLipid droplet formationLD biologyMitochondrial proteinsSecretory pathwayMass spectrometry analysisTerminal domainMitochondrial morphologyTransport proteinsLipid transportersCellular membranesLD metabolismMembrane contactX-ray structureSpectrometry analysisOrganellesGlycerophospholipidsProteinHydrophobic cavityFatty acidsLipidsMembraneDeciphering the molecular organization of GET pathway chaperones through native mass spectrometry
Giska F, Mariappan M, Bhattacharyya M, Gupta K. Deciphering the molecular organization of GET pathway chaperones through native mass spectrometry. Biophysical Journal 2022, 121: 1289-1298. PMID: 35189106, PMCID: PMC9034188, DOI: 10.1016/j.bpj.2022.02.026.Peer-Reviewed Original ResearchMeSH KeywordsCarrier ProteinsMass SpectrometryMolecular ChaperonesMultiprotein ComplexesSaccharomyces cerevisiae ProteinsUbiquitinConceptsNative mass spectrometryMultiprotein complexesOligomeric stateTail-anchored membrane proteinsLarge multiprotein complexesMass spectrometryChaperone complexMolecular modelingProtein complexesMutational perturbationsMembrane proteinsOligomeric organizationDimerization interfaceIndependent proteinsNative MSEndoplasmic reticulumUnstructured domainsConventional structure determinationStructural organizationMolecular organizationMolecular interfaceCyclic architectureProteinStructure determinationCrystal structure