2023
Sorting secretory proteins
Parchure A, von Blume J. Sorting secretory proteins. ELife 2023, 12: e93490. PMID: 37997893, PMCID: PMC10672786, DOI: 10.7554/elife.93490.Peer-Reviewed Original Research
2022
Quantification of Protein Exit at the Trans-Golgi Network
Tran M, Kim Y, von Blume J. Quantification of Protein Exit at the Trans-Golgi Network. Methods In Molecular Biology 2022, 2557: 583-594. PMID: 36512239, DOI: 10.1007/978-1-0716-2639-9_35.Peer-Reviewed Original ResearchMeSH KeywordsGolgi ApparatusHomeostasisProtein TransportProteinsSecretory PathwayTrans-Golgi NetworkConceptsTrans-Golgi networkProtein exportSecretory pathwaySelective hooks (RUSH) systemCell biological approachesProtein of interestPulse-chase experimentsProtein exitProtein sortingExport kineticsCellular homeostasisProtein transportDifferent cargoesLive cellsBiological approachesRUSH systemProteinPathwayTraffickingHomeostasisExportCargoSortingCompartmentsCellsLiquid–liquid phase separation facilitates the biogenesis of secretory storage granules
Parchure A, Tian M, Stalder D, Boyer C, Bearrows S, Rohli K, Zhang J, Rivera-Molina F, Ramazanov B, Mahata S, Wang Y, Stephens S, Gershlick D, von Blume J. Liquid–liquid phase separation facilitates the biogenesis of secretory storage granules. Journal Of Cell Biology 2022, 221: e202206132. PMID: 36173346, PMCID: PMC9526250, DOI: 10.1083/jcb.202206132.Peer-Reviewed Original ResearchMeSH KeywordsChromograninsCytoplasmic GranulesGolgi ApparatusHumansInsulinInsulin-Secreting CellsProinsulinSecretory VesiclesConceptsTrans-Golgi networkLiquid-liquid phase separationSecretory granulesClient moleculesSecretory storage granulesΒ-cellsMembrane traffickingCargo receptorsPlasma membranePancreatic β-cellsProcessing enzymesCell typesStorage granulesChromogranin proteinsBiogenesisAcidic pHTraffickingGranulesProteinEnzyme
2021
Cargo sorting at the trans-Golgi network at a glance
Ford C, Parchure A, von Blume J, Burd CG. Cargo sorting at the trans-Golgi network at a glance. Journal Of Cell Science 2021, 134 PMID: 34870705, PMCID: PMC8714066, DOI: 10.1242/jcs.259110.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneClathrin-Coated VesiclesGolgi ApparatusHumansProtein TransportTrans-Golgi NetworkConceptsTrans-Golgi networkDistinct export pathwaysClathrin-coated vesiclesTrafficking of glycoproteinsMechanism of proteinEndo-lysosomal organellesAccompanying posterGlance articleResident proteinsExport pathwayGolgi functionTrans-GolgiDifferent organellesGolgi cisternaeKey molecular featuresTrans cisternaeMembrane sacsFinal compartmentGolgiCell surfaceDistinct repertoireCell scienceTransport carriersProteinOrganellesCalcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells
Lebreton S, Paladino S, Liu D, Nitti M, von Blume J, Pinton P, Zurzolo C. Calcium levels in the Golgi complex regulate clustering and apical sorting of GPI-APs in polarized epithelial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2014709118. PMID: 34389665, PMCID: PMC8379914, DOI: 10.1073/pnas.2014709118.Peer-Reviewed Original ResearchConceptsApical sortingGPI-APsCholesterol-dependent clusteringGolgi-resident proteinsPlasma membrane organizationPolarized epithelial cellsEpithelial cellsDiverse vital functionsResident proteinsMolecular machinerySecretory cargoMembrane organizationGolgi eventUnexpected roleGolgi complexSpecific machineryGolgiBasolateral surfaceApical surfaceCab45SPCA1Vital functionsMachinerySortingProtein
2020
Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs
Pacheco-Fernandez N, Pakdel M, Blank B, Sanchez-Gonzalez I, Weber K, Tran ML, Hecht TK, Gautsch R, Beck G, Perez F, Hausser A, Linder S, von Blume J. Nucleobindin-1 regulates ECM degradation by promoting intra-Golgi trafficking of MMPs. Journal Of Cell Biology 2020, 219: e201907058. PMID: 32479594, PMCID: PMC7401813, DOI: 10.1083/jcb.201907058.Peer-Reviewed Original Research
2019
Signal peptide peptidase‐like 2c impairs vesicular transport and cleaves SNARE proteins
Papadopoulou A, Müller S, Mentrup T, Shmueli M, Niemeyer J, Haug‐Kröper M, von Blume J, Mayerhofer A, Feederle R, Schröder B, Lichtenthaler S, Fluhrer R. Signal peptide peptidase‐like 2c impairs vesicular transport and cleaves SNARE proteins. EMBO Reports 2019, 20: embr201846451. PMID: 30733281, PMCID: PMC6399617, DOI: 10.15252/embr.201846451.Peer-Reviewed Original ResearchMeSH KeywordsAcrosomeAnimalsAspartic Acid EndopeptidasesBiocatalysisDown-RegulationGlycomicsGlycoproteinsGlycosyltransferasesGolgi ApparatusHEK293 CellsHumansMaleMembrane ProteinsMice, Inbred C57BLModels, BiologicalProtein TransportProteolysisSNARE ProteinsSpermatidsSubcellular FractionsSubstrate SpecificityConceptsSNARE proteinsCandidate substratesVesicular transportProcess of vesicular traffickingImpaired vesicular transportIntramembrane aspartyl proteaseCleave SNARE proteinsGxGD-typeAcrosome formationB cell developmentCargo proteinsVesicular traffickingAspartyl proteaseProtease familyCellular processesProtein glycosylationSubcellular compartmentsEndoplasmic reticulumProteolytic processingBiological processesAlzheimer's diseasePhysiological functionsProteinDevelopment of pathologyProtease
2008
Protein kinase D2 regulates chromogranin A secretion in human BON neuroendocrine tumour cells
von Wichert G, Edenfeld T, von Blume J, Krisp H, Krndija D, Schmid H, Oswald F, Lother U, Walther P, Adler G, Seufferlein T. Protein kinase D2 regulates chromogranin A secretion in human BON neuroendocrine tumour cells. Cellular Signalling 2008, 20: 925-934. PMID: 18262756, DOI: 10.1016/j.cellsig.2008.01.003.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBiological Transport, ActiveCell Line, TumorChromogranin AGolgi ApparatusHumansMolecular Sequence DataNeuroendocrine TumorsProtein Kinase D2Protein Kinase InhibitorsProtein KinasesRNA, Small InterferingSecretory VesiclesSignal TransductionTrans-Golgi NetworkConceptsTrans-Golgi networkNeuroendocrine tumor cellsGolgi networkFunctional neuroendocrine tumorsProtein kinase D (PKD) familyGolgi apparatusProtein kinase D2Secretory granulesTumor cellsSerine-threonine kinaseHormone secretionNeuroendocrine tumorsChromogranin APerinuclear retentionThreonine kinaseSecretory vesiclesPrecise regulationAutonomous hormone secretionD family