2021
Cargo sorting at the trans-Golgi network at a glance
Ford C, Parchure A, von Blume J, Burd CG. Cargo sorting at the trans-Golgi network at a glance. Journal Of Cell Science 2021, 134 PMID: 34870705, PMCID: PMC8714066, DOI: 10.1242/jcs.259110.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneClathrin-Coated VesiclesGolgi ApparatusHumansProtein TransportTrans-Golgi NetworkConceptsTrans-Golgi networkDistinct export pathwaysClathrin-coated vesiclesTrafficking of glycoproteinsMechanism of proteinEndo-lysosomal organellesAccompanying posterGlance articleResident proteinsExport pathwayGolgi functionTrans-GolgiDifferent organellesGolgi cisternaeKey molecular featuresTrans cisternaeMembrane sacsFinal compartmentGolgiCell surfaceDistinct repertoireCell scienceTransport carriersProteinOrganellesSending out molecules from the TGN
Ramazanov BR, Tran ML, von Blume J. Sending out molecules from the TGN. Current Opinion In Cell Biology 2021, 71: 55-62. PMID: 33706234, PMCID: PMC8328904, DOI: 10.1016/j.ceb.2021.02.005.Peer-Reviewed Original ResearchMeSH KeywordsCell MembraneEndoplasmic ReticulumMembrane ProteinsProtein TransportTrans-Golgi NetworkConceptsTrans-Golgi networkEndoplasmic reticulum contact sitesCell-cell communicationExtracellular matrix proteinsMembrane traffickingCargo proteinsCargo sortingTGN structureTransmembrane proteinPlasma membraneContact sitesExciting linkSoluble proteinMatrix proteinsSorting mechanismProteinTransport carriersMajor open questionsSortingMembraneNovel typeTraffickingMachineryOrganismsVesicles
2019
Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death
Silvestre-Roig C, Braster Q, Wichapong K, Lee EY, Teulon JM, Berrebeh N, Winter J, Adrover JM, Santos GS, Froese A, Lemnitzer P, Ortega-Gómez A, Chevre R, Marschner J, Schumski A, Winter C, Perez-Olivares L, Pan C, Paulin N, Schoufour T, Hartwig H, González-Ramos S, Kamp F, Megens RTA, Mowen KA, Gunzer M, Maegdefessel L, Hackeng T, Lutgens E, Daemen M, von Blume J, Anders HJ, Nikolaev VO, Pellequer JL, Weber C, Hidalgo A, Nicolaes GAF, Wong GCL, Soehnlein O. Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death. Nature 2019, 569: 236-240. PMID: 31043745, PMCID: PMC6716525, DOI: 10.1038/s41586-019-1167-6.Peer-Reviewed Original ResearchConceptsSmooth muscle cellsChronic inflammationDestabilization of plaquesGlobal medical burdenImportant pathophysiological contributorCell deathPerpetuation of inflammationChronic vascular diseaseNeutrophil extracellular trapsMajor underlying causeLytic cell deathPathophysiological contributorVascular diseaseMedical burdenExtracellular trapsMouse modelAtherosclerotic lesionsMolecular mediatorsInflammationTissue damageTherapeutic valueMuscle cellsUnderlying causeDeathHistone H4
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivity
2014
Secretory cargo sorting at the trans-Golgi network
Kienzle C, von Blume J. Secretory cargo sorting at the trans-Golgi network. Trends In Cell Biology 2014, 24: 584-593. PMID: 24841758, DOI: 10.1016/j.tcb.2014.04.007.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkCytoplasmic actin cytoskeletonPlasma membrane deliverySubset of proteinsNovel sorting mechanismKey cellular eventsSecretory cargoActin cytoskeletonCellular eventsBinding proteinLysosomal hydrolasesSorting mechanismProteinNormal physiologySuccessful sortingRecent discoverySortingCab45CytoskeletonHydrolasesSPCA1ATPaseRegulationPhysiologyCargo
2012
Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast
Curwin AJ, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Molecular Biology Of The Cell 2012, 23: 2327-2338. PMID: 22553351, PMCID: PMC3374751, DOI: 10.1091/mbc.e11-09-0826.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsBlotting, WesternCathepsin ACell MembraneCofilin 1EndocytosisGlucan Endo-1,3-beta-D-GlucosidaseGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, FluorescenceMutationProtein TransportProton-Translocating ATPasesR-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrans-Golgi NetworkVacuolesConceptsTrans-Golgi networkLate Golgi membranesCarboxypeptidase YSecretory cargoGolgi membranesLate Golgi compartmentMechanisms of cargoSoluble secretory proteinsRole of cofilinATPase Pma1Yeast orthologueSynthetic sicknessProtein cofilinSpecific cargoMutant cellsMammalian cellsGolgi compartmentSorting defectsPlasma membraneSecretory proteinsGolgi apparatusATPase 1CofilinCell surfacePMR1