2024
Protocol for Imaging the Same Class IV Neurons at Different Stages of Development
Shree S, Howard J. Protocol for Imaging the Same Class IV Neurons at Different Stages of Development. Bio-protocol 2024, 14: e5052. PMID: 39210953, PMCID: PMC11349491, DOI: 10.21769/bioprotoc.5052.Peer-Reviewed Original ResearchClass IV neuronsClass IV dendritic arborizationInternal branchesInstar larval stageIV neuronsEgg layingSpinning disk confocal microscopeAgar padsDendritic arborizationClass I cellsDevelopmental stagesLarval stageLarval bodyArbor structureGrowth dynamicsSegment growthClass IV cellsLarvaeStages of developmentI cells
2022
Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations
Liao M, Kuo Y, Howard J. Counting fluorescently labeled proteins in tissues in the spinning–disk microscope using single–molecule calibrations. Molecular Biology Of The Cell 2022, 33: ar48. PMID: 35323029, PMCID: PMC9265152, DOI: 10.1091/mbc.e21-12-0618.Peer-Reviewed Original ResearchConceptsEnd-binding protein 1Spinning-disk confocal microscopyConfocal microscopySingle-molecule imagingComplex biological phenomenaFly larvaeLiving cellsCell surfaceMicrotubule endsBiological phenomenaProtein 1Brightness of fluorophoresCytoplasmic concentrationEpifluorescence microscopeBiological systemsSensory neuronsCellsSingle moleculesAbsolute numberMolecular numberTissueLarvaeMicrotubulesEGFPProtein