2004
Pre-Steady-State Kinetics of RB69 DNA Polymerase and Its Exo Domain Mutants: Effect of pH and Thiophosphoryl Linkages on 3‘−5‘ Exonuclease Activity †
Wang C, Zakharova E, Li J, Joyce C, Wang J, Konigsberg W. Pre-Steady-State Kinetics of RB69 DNA Polymerase and Its Exo Domain Mutants: Effect of pH and Thiophosphoryl Linkages on 3‘−5‘ Exonuclease Activity †. Biochemistry 2004, 43: 3853-3861. PMID: 15049692, DOI: 10.1021/bi0302292.Peer-Reviewed Original ResearchMeSH KeywordsAlanineAmino Acid SubstitutionBacteriophage T4Base Pair MismatchDNA Polymerase IDNA-Directed DNA PolymeraseEnzyme ActivationExodeoxyribonucleasesGlutamineHydrogen-Ion ConcentrationKineticsMutagenesis, Site-DirectedPhosphatesPhosphorylationProtein Structure, TertiaryRNA EditingSubstrate SpecificityThionucleotidesT-PhagesViral ProteinsConceptsRate-determining stepDivalent metal ionsPH-activity profileB family replicative DNA polymerasesChemical stepMetal ionsSingle-turnover conditionsWild-type enzymeEffects of pHKlenow fragmentB-family DNA polymerasesFamily DNA polymerasesState kineticsDNA polymeraseThree-dimensional structureDomain mutantsExonuclease reactionExonuclease activityPhosphorothioate linkagesPhi29 DNA polymeraseElemental effectsReplicative DNA polymerasesRepair DNA polymerasesExo activityCatalysis
2001
Structure of the Replicating Complex of a Pol α Family DNA Polymerase
Franklin M, Wang J, Steitz T. Structure of the Replicating Complex of a Pol α Family DNA Polymerase. Cell 2001, 105: 657-667. PMID: 11389835, DOI: 10.1016/s0092-8674(01)00367-1.Peer-Reviewed Original ResearchConceptsAlpha familyDNA polymeraseResolution crystal structureTernary complex structureApo-protein structurePrimer-template DNAMinor groove interactionsFamily DNA polymerasesFamily polymerasesRB69 DNA polymerasePol IFidelity mechanismsPrimer 3' terminusPrimer extensionPolymeraseGroove interactionsDNA motionTerminusDNA orientationFamilyDNADegrees rotationCrystal structureComplex structureDTTP
1997
Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69
Wang J, Sattar A, Wang C, Karam J, Konigsberg W, Steitz T. Crystal Structure of a pol α Family Replication DNA Polymerase from Bacteriophage RB69. Cell 1997, 89: 1087-1099. PMID: 9215631, DOI: 10.1016/s0092-8674(00)80296-2.Peer-Reviewed Original ResearchBacteriophagesBinding SitesConserved SequenceCrystallographyDNA Polymerase IDNA Polymerase IIDNA, Single-StrandedEscherichia coliExonucleasesGene Expression Regulation, ViralHIVProtein ConformationProtein Structure, SecondaryProtein Structure, TertiaryReplication OriginRNA-Directed DNA PolymeraseSequence Homology, Amino Acid
1995
Crystal structure of Thermus aquaticus DNA polymerase
Kim Y, Eom S, Wang J, Lee D, Suh S, Steitz T. Crystal structure of Thermus aquaticus DNA polymerase. Nature 1995, 376: 612-616. PMID: 7637814, DOI: 10.1038/376612a0.Peer-Reviewed Original Research
1993
Two DNA polymerases: HIV reverse transcriptase and the Klenow fragment of Escherichia coli DNA polymerase I.
Steitz T, Smerdon S, Jäger J, Wang J, Kohlstaedt L, Friedman J, Beese L, Rice P. Two DNA polymerases: HIV reverse transcriptase and the Klenow fragment of Escherichia coli DNA polymerase I. Cold Spring Harbor Symposia On Quantitative Biology 1993, 58: 495-504. PMID: 7525146, DOI: 10.1101/sqb.1993.058.01.056.Peer-Reviewed Original Research
1992
Crystal Structure at 3.5 Å Resolution of HIV-1 Reverse Transcriptase Complexed with an Inhibitor
Kohlstaedt L, Wang J, Friedman J, Rice P, Steitz T. Crystal Structure at 3.5 Å Resolution of HIV-1 Reverse Transcriptase Complexed with an Inhibitor. Science 1992, 256: 1783-1790. PMID: 1377403, DOI: 10.1126/science.1377403.Peer-Reviewed Original Research