2009
RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity
Zhang H, Beckman J, Wang J, Konigsberg W. RB69 DNA Polymerase Mutants with Expanded Nascent Base-Pair-Binding Pockets Are Highly Efficient but Have Reduced Base Selectivity. Biochemistry 2009, 48: 6940-6950. PMID: 19522539, PMCID: PMC2847438, DOI: 10.1021/bi900422b.Peer-Reviewed Original ResearchConceptsBase pairsCorrect base pairReplicative DNA polymerasesRB69 polRB69 DNA Polymerase MutantsNascent base pairDouble mutantSingle mutantsTriple mutantNumber of substitutionsWild typeMutantsBacteriophage RB69DNA polymerase mutantsPolymerase mutantsDNA polymeraseBinding pocketsNegative selectionDNA polRapid incorporationCatalytic centerLow incorporation efficiencyG mutationSulfolobus solfataricus Dpo4Base discrimination
2008
Structural basis for base discrimination by RB69 DNA polymerase
Wang M, Klimenko D, Steitz T, Wang J. Structural basis for base discrimination by RB69 DNA polymerase. The FASEB Journal 2008, 22: 593.2-593.2. DOI: 10.1096/fasebj.22.1_supplement.593.2.Peer-Reviewed Original ResearchTriple mutantApo formStructural basisBase pairsDNA polymeraseReplicative DNA polymerasesWild-type enzymeTernary complexTemplating baseHelix PBase selectivityNascent base pairRB69 DNA polymeraseBase discriminationWild-type PolType enzymeMismatched base pairsMutantsPol mutantsRB69 polPolymeraseComplexesS565Y416Pol