2023
Structure-based discovery of conformationally selective inhibitors of the serotonin transporter
Singh I, Seth A, Billesbølle C, Braz J, Rodriguiz R, Roy K, Bekele B, Craik V, Huang X, Boytsov D, Pogorelov V, Lak P, O'Donnell H, Sandtner W, Irwin J, Roth B, Basbaum A, Wetsel W, Manglik A, Shoichet B, Rudnick G. Structure-based discovery of conformationally selective inhibitors of the serotonin transporter. Cell 2023, 186: 2160-2175.e17. PMID: 37137306, PMCID: PMC10306110, DOI: 10.1016/j.cell.2023.04.010.Peer-Reviewed Original Research
2013
Isoforms of the neuronal glutamate transporter gene, SLC1A1/EAAC1, negatively modulate glutamate uptake: relevance to obsessive-compulsive disorder
Porton B, Greenberg B, Askland K, Serra L, Gesmonde J, Rudnick G, Rasmussen S, Kao H. Isoforms of the neuronal glutamate transporter gene, SLC1A1/EAAC1, negatively modulate glutamate uptake: relevance to obsessive-compulsive disorder. Translational Psychiatry 2013, 3: e259-e259. PMID: 23695234, PMCID: PMC3669922, DOI: 10.1038/tp.2013.35.Peer-Reviewed Original ResearchConceptsExcellent candidate geneObsessive-compulsive disorderNeuronal glutamate transporterGenomic levelInternal promoterCandidate genesPartial colocalizationTransporter geneGene expressionGenetic studiesGlutamate uptakeEAAC1 transporterPrimary promoterEAAC1 proteinGlutamate transporter geneGenesPhysiological regulatorIsoformsTranscriptsExon 2Glutamate transportersGlutamatergic gene expressionPromoterPotential clinical utilityNeuronal glutamate transporter gene
2001
The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells
Gu H, Wu X, Giros B, Caron M, Caplan M, Rudnick G. The NH2-terminus of Norepinephrine Transporter Contains a Basolateral Localization Signal for Epithelial Cells. Molecular Biology Of The Cell 2001, 12: 3797-3807. PMID: 11739781, PMCID: PMC60756, DOI: 10.1091/mbc.12.12.3797.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCell LineCell PolarityDogsDopamine Plasma Membrane Transport ProteinsEpithelial CellsHumansMembrane GlycoproteinsMembrane Transport ProteinsMiceMicroscopy, ConfocalMolecular Sequence DataMutagenesis, Site-DirectedNerve Tissue ProteinsNorepinephrine Plasma Membrane Transport ProteinsProtein Sorting SignalsSequence AlignmentSymportersConceptsBasolateral localization signalLocalization signalDileucine motifPlasma membraneBasolateral localizationOverall amino acid sequence identityAmino acid sequence identityTerminal regionMDCK cellsApical plasma membraneBasolateral membraneEpithelial cellsSequence identityApical localizationChimeric proteinTransport assaysTransporter localizationAmino acidsApical membraneNorepinephrine transporterTransportersCorresponding sequenceDopamine transporterSame mutationMembrane
1992
Expression of a cloned gamma-aminobutyric acid transporter in mammalian cells.
Keynan S, Suh Y, Kanner B, Rudnick G. Expression of a cloned gamma-aminobutyric acid transporter in mammalian cells. Biochemistry 1992, 31: 1974-9. PMID: 1536839, DOI: 10.1021/bi00122a011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCarrier ProteinsChloridesCloning, MolecularDNAGABA Plasma Membrane Transport ProteinsGamma-Aminobutyric AcidGene ExpressionGenetic VectorsHeLa CellsHumansKineticsL CellsMembrane ProteinsMembrane Transport ProteinsMiceNerve Tissue ProteinsOrganic Anion TransportersPlasmidsPrecipitin TestsRatsSodiumTransfectionTunicamycinXenopusConceptsMammalian cellsGABA transportMouse Ltk- cellsT7 RNA polymerasePlasma membrane vesiclesL cellsApparent molecular massGABA transporterSynaptic plasma membrane vesiclesGamma-aminobutyric acid transporterPresence of tunicamycinEukaryotic expression vectorRNA polymeraseTransient expressionExpression vectorAcid transportersMembrane vesiclesStable expressionLtk- cellsFunctional expressionGAT-1Molecular massHeLa cellsTransportersTransfection
1983
Acidification of macrophage and fibroblast endocytic vesicles in vitro.
Galloway C, Dean G, Marsh M, Rudnick G, Mellman I. Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings Of The National Academy Of Sciences Of The United States Of America 1983, 80: 3334-3338. PMID: 6190176, PMCID: PMC394037, DOI: 10.1073/pnas.80.11.3334.Peer-Reviewed Original ResearchConceptsAcidic prelysosomal compartmentEndosome-lysosome fusionATP-dependent acidificationF0-ATPaseEndocytic vesiclesPrelysosomal compartmentAddition of ATPPrelysosomal vacuolesMitochondrial F1LysosomesEndosomesSecretory granulesCultured macrophagesN-ethylmaleimideInternal pHPercoll density gradientsAcidificationPH gradientFibroblastsCompartmentsOrganellesInhibitorsPermeant anionsFluorescein-dextranVacuoles