2023
SLC6 neurotransmitter transporter family in GtoPdb v.2023.1
Bröer S, Rudnick G. SLC6 neurotransmitter transporter family in GtoPdb v.2023.1. IUPHAR/BPS Guide To Pharmacology CITE 2023, 2023 DOI: 10.2218/gtopdb/f144/2023.1.Peer-Reviewed Original ResearchSolute carrier family 6Transporter familyNeurotransmitter transporter familyDependent neurotransmitter transportersAmino acid transportersDependent amino acid transporterNSS transportersTM segmentsPlasma membraneNeurotransmitter transportersAcid transportersFamily 6Neutral amino acidsAmino acidsTransportersStructural motifsGtoPdb v.LeuTAeolicusSubfamiliesFamilyLeuTAaMembersMotifCrystal structure
2019
SLC6 neurotransmitter transporter family (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database
Bröer S, Rudnick G. SLC6 neurotransmitter transporter family (version 2019.4) in the IUPHAR/BPS Guide to Pharmacology Database. IUPHAR/BPS Guide To Pharmacology CITE 2019, 2019 DOI: 10.2218/gtopdb/f144/2019.4.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsSolute carrier family 6Transporter familyNeurotransmitter transporter familyDependent neurotransmitter transportersAmino acid transportersDependent amino acid transporterNSS transportersTM segmentsPlasma membraneNeurotransmitter transportersAcid transportersFamily 6Neutral amino acidsIUPHAR/BPS GuideAmino acidsTransportersStructural motifsLeuTAeolicusSubfamiliesFamilyPharmacology DatabaseLeuTAaMembersMotif
2015
Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog*
Tavoulari S, Margheritis E, Nagarajan A, DeWitt DC, Zhang YW, Rosado E, Ravera S, Rhoades E, Forrest LR, Rudnick G. Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog*. Journal Of Biological Chemistry 2015, 291: 1456-1471. PMID: 26582198, PMCID: PMC4714228, DOI: 10.1074/jbc.m115.692012.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAmino Acid Transport SystemsAquatic OrganismsBacterial ProteinsBinding SitesCysteineGram-Negative BacteriaLigandsLiposomesModels, MolecularMolecular Dynamics SimulationMutagenesis, Site-DirectedMutationPlasma Membrane Neurotransmitter Transport ProteinsProtein ConformationProtein FoldingProtein StabilityProteolipidsRecombinant ProteinsSodiumConceptsConformational changesTransmembrane helix 1Open conformational stateDependent conformational changesTransporter homologExtracellular gateProkaryotic homologCytoplasmic pathwayHelix 1Interaction networksIntermediary interactionsBiophysical assaysNeurotransmitter transportersSubstrate pathwayNa2 siteConformational statesHelix motionsLeuTDirect interactionDependent closureHomologMutantsDistinct stepsResiduesComputational analysis
2011
Cytoplasmic Permeation Pathway of Neurotransmitter Transporters
Rudnick G. Cytoplasmic Permeation Pathway of Neurotransmitter Transporters. Biochemistry 2011, 50: 7462-7475. PMID: 21774491, PMCID: PMC3164596, DOI: 10.1021/bi200926b.Peer-Reviewed Original ResearchConceptsCytoplasmic permeation pathwaysBacterial amino acid transporter LeuTNeurotransmitter transportersPermeation pathwayKingdoms of lifeMammalian serotonin transporterHigh-resolution crystal structuresFour-helix bundleRecent high-resolution crystal structureSubsequent crystal structureStructural repeatsLeuT structureProtein familyCommon structural featuresSolute transportersRelated proteinsLarge structural familyCytoplasmic oneConformational changesSubstrate siteFirst structureBiological membranesTransportersLeuTExtracellular pathways
2010
Reconstructing a Chloride-binding Site in a Bacterial Neurotransmitter Transporter Homologue*
Tavoulari S, Rizwan AN, Forrest LR, Rudnick G. Reconstructing a Chloride-binding Site in a Bacterial Neurotransmitter Transporter Homologue*. Journal Of Biological Chemistry 2010, 286: 2834-2842. PMID: 21115480, PMCID: PMC3024779, DOI: 10.1074/jbc.m110.186064.Peer-Reviewed Original ResearchConceptsChloride-binding siteConformational changesAdjacent binding sitesSingle point mutationProkaryotic homologuesSubstrate translocationIon-binding sitesTransporter homologueTransport proteinsNeurotransmitter transportersNeurotransmitter transportPoint mutationsBinding sitesHomologuesProteinMutationsCl(-) bindsDirect evidenceTherapeutic drugsSitesDependent formTranslocationTransportersBindsResidues
2008
Mechanism for alternating access in neurotransmitter transporters
Forrest LR, Zhang YW, Jacobs MT, Gesmonde J, Xie L, Honig BH, Rudnick G. Mechanism for alternating access in neurotransmitter transporters. Proceedings Of The National Academy Of Sciences Of The United States Of America 2008, 105: 10338-10343. PMID: 18647834, PMCID: PMC2480614, DOI: 10.1073/pnas.0804659105.Peer-Reviewed Original ResearchConceptsNeurotransmitter transportersMammalian neurotransmitter transportersMammalian serotonin transporterTransmembrane helix 1Bacterial homologueIon-binding sitesTransporter familyExtensive mutagenesisHelix 1Similar repeatsLeuTConformational changesSerotonin transporterRepeatsAlternate conformationConformational differencesExtracellular pathwaysCytoplasmTransportersExtracellular spaceCysteine reagentCrystal structureConformationPathwayAccessibility measurements
2003
Characterization of a functional bacterial homologue of sodium-dependent neurotransmitter transporters.
Androutsellis-Theotokis A, Goldberg N, Ueda K, Beppu T, Beckman M, Das S, Javitch J, Rudnick G. Characterization of a functional bacterial homologue of sodium-dependent neurotransmitter transporters. Journal Of Biological Chemistry 2003, 278: 26302. DOI: 10.1016/s0021-9258(19)75165-5.Peer-Reviewed Original ResearchSodium-dependent neurotransmitter transportersNeurotransmitter transporters
1999
Molecular cloning, expression and characterization of a bovine serotonin transporter1The sequence reported in this paper has been deposited in the GenBank data base (accession number AF119122).1
Mortensen O, Kristensen A, Rudnick G, Wiborg O. Molecular cloning, expression and characterization of a bovine serotonin transporter1The sequence reported in this paper has been deposited in the GenBank data base (accession number AF119122).1. Brain Research 1999, 71: 120-126. PMID: 10407194, DOI: 10.1016/s0169-328x(99)00178-3.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCarrier ProteinsCattleCitalopramCloning, MolecularDesipramineFemaleFluoxetineHeLa CellsHumansImipramineKineticsMembrane GlycoproteinsMembrane Transport ProteinsMolecular Sequence DataNerve Tissue ProteinsN-Methyl-3,4-methylenedioxyamphetamineOrgan SpecificityParoxetinePhylogenyPregnancyRatsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSequence AlignmentSequence Homology, Amino AcidSerotoninSerotonin Plasma Membrane Transport ProteinsTransfectionConceptsSerotonin transporterHuman serotonin transporterExpression of SERTAdrenal glandBrain stemParathyroid glandsPharmacological profileBone marrowThyroid glandSmall intestinePharmacological targetsRT-PCR amplificationDecreased sensitivityExtracellular fluidGlandAmino acid differencesBiogenic aminesNeurotransmitter transportersDependent neurotransmitter transportersImportant antidepressantsAcid differencesDifferent tissuesAntidepressantsParoxetineDesipramine
1998
Bioenergetics of Neurotransmitter Transport
Rudnick G. Bioenergetics of Neurotransmitter Transport. Journal Of Bioenergetics And Biomembranes 1998, 30: 173-185. PMID: 9672239, DOI: 10.1023/a:1020573325823.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsSynaptic vesicle membranePlasma membrane transportersRecycling of neurotransmittersGene familyVesicle lumenPlasma membraneMembrane transportersNeurotransmitter transportersVesicle membraneNeurotransmitter transportVesicular transportersAmino acidsTransportersCoupling stoichiometryDownhill movementCytoplasmEssential componentUphill transportFamilyMembraneEffluxBioenergeticsImportant drugsProteinInflux[16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry
Rudnick G. [16] Ion-coupled neurotransmitter transport: Thermodynamic vs. kinetic determinations of stoichiometry. Methods In Enzymology 1998, 296: 233-247. PMID: 9779452, DOI: 10.1016/s0076-6879(98)96018-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsNumber of ionsKinetic determinationMolecular machinesCharge movesIonsLipid bilayersNeurotransmitter moleculesMoleculesInput of energySteady-state distributionLow concentrationsChargeElectrical potentialMembraneIon gradientsStoichiometryHigh concentrationsUltimate concentrationNaConcentrationEnergyBilayersNeurotransmitter transportersNumber of NaNeurotransmitter transport
1996
Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells*
Gu H, Ahn J, Caplan M, Blakely R, Levey A, Rudnick G. Cell-specific Sorting of Biogenic Amine Transporters Expressed in Epithelial Cells*. Journal Of Biological Chemistry 1996, 271: 18100-18106. PMID: 8663573, DOI: 10.1074/jbc.271.30.18100.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiogenic AminesBiological TransportCarrier ProteinsCell CompartmentationCell MembraneCell PolarityCells, CulturedDogsDopamineDopamine Plasma Membrane Transport ProteinsEpithelial CellsHumansImmunohistochemistryMembrane GlycoproteinsMembrane Transport ProteinsNerve Tissue ProteinsNorepinephrineNorepinephrine Plasma Membrane Transport ProteinsRatsRecombinant ProteinsSerotoninSerotonin Plasma Membrane Transport ProteinsSymportersConceptsMadin-Darby canine kidneyMDCK cellsLLC-PK1 cellsNeurotransmitter transportersCell surface biotinylationConfocal immunofluorescence microscopyBasolateral membraneCell-specific mechanismsEpithelial cellsBiogenic amine transportersMembrane proteinsSurface biotinylationCDNA encodingHuman DA transporterAmine transportersImmunofluorescence microscopyBiotinylating reagentTransportersPermeable filter supportsApical surfaceImmunocytochemistry resultsBasolateral mediumSurface expressionApical sideDA transporterChapter 17 Biogenic amine transporters of the plasma membrane
Rudnick G. Chapter 17 Biogenic amine transporters of the plasma membrane. Handbook Of Biological Physics 1996, 2: 381-404. DOI: 10.1016/s1383-8121(96)80058-3.ChaptersPlasma membraneSpecific transportersSmall neurotransmittersNerve terminalsBiogenic amine transportersMain inactivation mechanismNeurotransmitter transportersExtracellular transmitterAmine transportersTransportersBiogenic amine neurotransmittersInactivation mechanismAntidepressant drugsNervous systemNeurotransmitter activityCases of ACHNeurotransmittersAmine neurotransmittersProducts of hydrolysisMembraneCellsNorepinephrineAChDopamineHydrolysis